protein

Mouse Growth Hormone Recombinant

MBS553233

0.01 mg

145 USD

Recombinant Protein

Mouse Growth Hormone Recombinant

Growth Hormone

Somatotropin; gh1; gh-1; growth hormone

somatotropin; Somatotropin; Growth hormone

GH1

Gh1; Gh

SOMA_MOUSE

E Coli

216

MATDSRTSWL LTVSLLCLLW PQEASAFPAM PLSSLFSNAV LRAQHLHQLA ADTYKEFERA YIPEGQRYSI QNAQAAFCFS ETIPAPTGKE EAQQRTDMEL LRFSLLLIQS WLGPVQFLSR IFTNSLMFGT SDRVYEKLKD LEEGIQALMQ ELEDGSPRVG QILKQTYDKF DANMRSDDAL LKNYGLLSCF KKDLHKAETY LRVMKCRRFV ESSCAF

>95%, as determined by SDS-PAGE and HPLC

Recombinant Mouse Growth Hormone was lyophilized from 0.2 um filtered 100mM NaCl, 20mM PB, pH 7.4.

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Domain: four helix cytokine core. Host Note: Optimized DNA sequence encoding Mouse Growth Hormone mature chain was expressed in Escherichia Coli.

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Molecular Weight Note: Native Mouse Growth Hormone is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 22 kDa. Recombinant GH is a disulfide-linked homodimeric protein consisting of 191 amino acid residue subunits, and migrates as an approximately 22 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

Cytokines

The physiological activity for which growth hormone is best known is the promotion of growth of bone, cartilage, and soft tissues. Detectable levels of growth hormone are found throughout the remainder of adulthood, suggesting other functions in addition to promotion of growth. Growth hormone may be important for the maintenance of lean body mass; most growth-promoting effects of growth hormone are mediated by IGF-1 the synthesis of which is regulated by growth hormone. The biological activities of growth hormone are mediated by receptors belonging to one of the Cytokine receptor families. Growth hormone has been shown to be produced by T- cells, B-cells, and macrophages. In human lymphocytes growth hormone appears to up-regulate its own expression. Growth hormone appears to act as an enhancer of immune responses and is produced in considerable amounts by T- helper cells.

Growth Hormone secretagogues and Growth Hormone releasing peptides act as orthosteric super-agonists but not allosteric regulators for activation of the g protein go1 by the ghrelin receptor . Mol. Pharmacol., Oct 2009; 76: 802 - 811. statement by the Growth Hormone research society on the gh/igf-i axis in extending health span . J Gerontol A Biol Sci Med Sci, Oct 2009; 64A: 1039 - 1044. the p561t polymorphism of the Growth Hormone receptor gene has an inhibitory effect on mandibular growth in young children . Eur J Orthod, Oct 2009; 31: 536 - 541. central ghrelin regulates peripheral lipid metabolism in a Growth Hormone -independent fashion . Endocrinology, Oct 2009; 150: 4562 - 4574. effects of syndyphalin-33 on feed intake and circulating measures of Growth Hormone , cortisol, and immune cell populations in the recently weaned pig . J Anim Sci, Oct 2009; 87: 3218 - 3225.

6679997

NP_032143.1

NM_008117.3

P06880

24,716 Da

Adipogenesis Pathway (198299); Diabetes Pathways (367029); Jak-STAT Signaling Pathway (83274); Jak-STAT Signaling Pathway (488); Neuroactive Ligand-receptor Interaction Pathway (83250); Neuroactive Ligand-receptor Interaction Pathway (462); Synthesis, Secretion, And Deacylation Of Ghrelin Pathway (367046)

GH: Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A); also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH. Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B); also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH. Defects in GH1 are the cause of Kowarski syndrome (KWKS); also known as pituitary dwarfism VI. Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2). IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy. Belongs to the somatotropin/prolactin family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Hormone; Secreted, signal peptide. Cellular Component: extracellular space; mitochondrion; plasma membrane; extracellular region; trans-Golgi network; cytosol; nucleus; secretory granule. Molecular Function: growth hormone receptor binding; metal ion binding; hormone activity. Biological Process: response to food; response to light stimulus; positive regulation of neurogenesis; cellular response to insulin stimulus; neuroblast proliferation; glucose transport; positive regulation of multicellular organism growth; regulation of steroid hormone receptor signaling pathway; signal transduction; positive regulation of growth; alveolus development

0.01 mg

145 USD

0.05 mg

220