protein

Human Interferon alphacon-1 Recombinant

MBS553232

0.01 mg

145 USD

Recombinant Protein

Human Interferon alphacon-1 Recombinant

Interferon alphacon-1

Human Interferon alphacon 1 Recombinant; ifna; interferon alpha; ifn-a

interferon alpha-2; Interferon alpha-2; Interferon alpha-A

IFN-a con

IFN-alpha

IFNA2; IFN-alpha-2; LeIF A

IFNA2_HUMAN

E Coli

188

MALTFALLVA LLVLSCKSSC SVGCDLPQTH SLGSRRTLML LAQMRKISLF SCLKDRHDFG FPQEEFGNQF QKAETIPVLH EMIQQIFNLF STKDSSAAWD ETLLDKFYTE LYQQLNDLEA CVIQGVGVTE TPLMKEDSIL AVRKYFQRIT LYLKEKKYSP CAWEVVRAEI MRSFSLSTNL QESLRSKE

>97%, as determined by SDS-PAGE and HPLC

Recombinant Interferon alpha 2a was lyophilized from a 0.2 um filtered PBS solution pH 7.0.

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Domain: four helix cytokine core. Host Note: Optimized DNA sequence encoding Human Interferon-alpha concensus mature chain was expressed in Escherichia Coli.

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Molecular Weight Note: Recombinant Interferon alpha concensus is a disulfide-linked monomeric protein consisting of 166 amino acid residue subunits, and migrates as an approximately 19 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.

Cytokines

All known subtypes of IFN- alpha show the same antiviral antiparasitic, antiproliferative activities. Human IFN-alpha is also a potent antiviral substance in murine, porcine, and bovine cell systems. IFN-alpha forms are produced by monocytes/macrophages, lymphoblastoid cells, fibroblasts, and a number of different cell types following induction by viruses, nucleic acids, glucocorticoid hormones, and low-molecular weight substances. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino- terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. IFN-alpha and IFN-beta are thought to bind to the same receptor. Signal transduction mechanisms elicited after binding of IFN-alpha to its receptors involves tyrosine phosphorylation (see also: PTK; protein tyrosine kinase) of various non- receptor tyrosine kinases belonging to the Janus kinases.

cytokines and angiogenic factors in patients with metastatic renal cell carcinoma treated with interferon-: association of pretreatment serum levels with survival . Ann. Onc., Oct 2009; 20: 1682 - 1687. sequential multiple-assignment randomized trial design of neurobehavioral treatment for patients with metastatic malignant melanoma undergoing high-dose interferon-alpha therapy . Clinical Trials, Sep 2009; 10.1177/1740774509344633. role of schlafen 2 (slfn2) in the generation of interferon -induced growth inhibitory responses . J. Biol. Chem., Sep 2009; 284: 25051 - 25064. palmitoylation of interferon- (ifn-) receptor subunit ifnar1 is required for the activation of stat1 and stat2 by ifn- . J. Biol. Chem., Sep 2009; 284: 24328 - 24340. lack of association between the interferon- signature and longitudinal changes in disease activity in systemic lupus erythematosus . Ann Rheum Dis, Sep 2009; 68: 1440 - 1446.

11067751

NP_000596.2

NM_000605.3

P01563

21,550 Da

Autoimmune Thyroid Disease Pathway 83121!!Autoimmune Thyroid Disease Pathway 533!!Cytokine Signaling In Immune System Pathway 366171!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Cytosolic DNA-sensing Pathway 125137!!Cytosolic DNA-sensing Pathway 124833!!Factors Involved In Megakaryocyte Development And Platelet Production Pathway 187196!!Hemostasis Pathway 106028!!Hepatitis C Pathway 173973

Function: Produced by macrophages, IFN-alpha have antiviral activities. Subunit structure: Interacts with IFNAR2. Ref.16 Ref.17. Subcellular location: Secreted. Polymorphism: Three forms exist; alpha-2a (shown here), alpha-2b and alpha-2c. Pharmaceutical use: Available under the names Roferon-A (Roche) or Intron-A (Schering-Plough). Used as an anticancer drug for its antiproliferative activity. Sequence similarities: Belongs to the alpha/beta interferon family.

0.01 mg

145 USD