protein

Human Neuregulin-4 Recombinant

MBS553227

0.02 mg

345 USD

Recombinant Protein

Human Neuregulin-4 Recombinant

Neuregulin-4

egf; neuregulin; nrg4; nrg-4; heregulin; hrg

pro-neuregulin-4, membrane-bound isoform; Pro-neuregulin-4, membrane-bound isoform

NRG4

NRG4; Pro-NRG4; NRG-4

NRG4_HUMAN

E Coli

115

MPTDHEEPCG PSHKSFCLNG GLCYVIPTIP SPFCRCVENY TGARCEEVFL PGSSIQTKSN LFEAFVALAV LVTLIIGAFY FLCRKGHFQR ASSVQYDINL VETSSTSAHH SHEQH

>96% as determined by SDS-PAGE and HPLC

Recombinant Neuregulin-4 was lyophilized from a 0.2 um filtered 20mM PB, pH 6.0.

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Domain: EGF. Host Note: Optimized DNA sequence encoding Human Neuregulin-4 EGF domain was expressed in Escherichia Coli. Biological Activity:: The ED(50) was determined by the phosphorylation of ErbB2 and ErbB4 receptors in CHO cells, and was found to be <3ng.ml, corresponding to a specific activity of 3x 10^5 Units/mg. Presentation: Recombinant Neuregulin-4 was lyophilized from a 0.2 f.lm filtered 20mM PB, pH 6.0.

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Cytokines

Neuregulins (NDF, heregulin, GGF ARIA, or SMDF) are EGF- like growth and differentiation factors that signal through tyrosine kinase receptors of the ErbB family.The ErbB2 and ErbB4 receptors cooperate in transmission of neuregulin-1 signals in the heart, whereas ErbB2 and ErbB3 cooperate in neural crest cells.

expression of neuregulin 4 splice variants in normal human tissues and prostate cancer and their effects on cell motility . Endocr. Relat. Cancer, Dec 2010; 18: 39 - 49.

20070380

NP_612640.1

NM_138573.3

Q8WWG1

Recombinant human Neuregulin-4 EGF domain is a disulfide-linked monomeric protein consisting of 62 amino acid residue subunits. and migrates as an approximately 7 kDa protein under non-reducing and reducing conditions in SOS-PAGE.

ErbB Receptor Signaling Network Pathway (138016); ErbB Signaling Pathway (198844); ErbB Signaling Pathway (83049); ErbB Signaling Pathway (458); ErbB4 Signaling Events Pathway (137991)

The neuregulins, including NRG4, activate type-1 growth factor receptors (see EGFR; MIM 131550) to initiating cell-to-cell signaling through tyrosine phosphorylation (Harari et al., 1999 [PubMed 10348342]).[supplied by OMIM, Mar 2008]

NRG4: Low affinity ligand for the ERBB4 tyrosine kinase receptor. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. Does not bind to the ERBB1, ERBB2 and ERBB3 receptors. Belongs to the neuregulin family. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 15q24.2. Cellular Component: extracellular space; integral to membrane; extracellular region; plasma membrane. Molecular Function: growth factor activity; receptor binding. Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; organ development; innate immune response

0.02 mg

345 USD

0.05 mg

595