protein

Human CD155 Recombinant

MBS553217

0.02 mg

220 USD

Recombinant Protein

Human CD155 Recombinant

CD155

cd-155; PVR; CD155; HVED; NECL5; Necl-5; PVS; TAGE4

poliovirus receptor isoform beta; Poliovirus receptor; Nectin-like protein 5

CD155

PVR; PVS; NECL-5

PVR_HUMAN

HEk293 Cells

372

>95%, as determined by SDS-PAGE and HPLC

Recombinant CD155 is supplied as a 0.2 um filtered PBS solution, pH7.2.

Recombinant CD155, as supplied, can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C to -70 degree C for twelve months. Avoid repeated freeze/thaw cycles.

Domain: Ig-like C2. Host Note: Optimized DNA sequence encoding Human extracellular domain of human CD155 including a C-terminal 6His tag was expressed in HEK293 cells. Biological Activity: The biological activity of Human PVR was measured by the binding ability of immobilized recombinant Human CD155 (2ug/ml, 100 ul/well) to Human DNAM1 (linear rande of 1-32 ng/ml) in a functional ELISA assay. Reconsitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1mg/ml. This solution can then be diluted into other buffers.

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Molecular Weight Note: Recombinant CD155 is a monomer protein consisting of 329 amino acid residue subunits, due to glycosylation migrates as an approximately 55-60 kDa protein on SDS-PAGE.

Receptors CD

hPVR (CD155) is a member of the immunoglobulin (Ig) superfamily, with three linked extracellular Ig-like domains followed by a membrane-spanning domain and a cytoplasmic domain. Although a physiological function for CD155 is unknown, the protein binds specifically to the extracellular matrix component vitronectin (Lange et al., 2001). Transcriptional regulation of the CD155 gene is tightly controlled.

dnam-1/ CD155 interactions promote cytokine and nk cell-mediated suppression of poorly immunogenic melanoma metastases . J. Immunol., Jan 2010; 184: 902 - 911. primary human tumor cells expressing CD155 impair tumor targeting by down-regulating dnam-1 on nk cells . J. Immunol., Oct 2009; 183: 4921 - 4930. cd96 interaction with CD155 via its first ig-like domain is modulated by alternative splicing or mutations in distal ig-like domains . J. Biol. Chem., Jan 2009; 284: 2235 - 2244. crystal structure of CD155 and electron microscopic studies of its complexes with polioviruses . PNAS, Nov 2008; 105: 18284 - 18289. characterization of the new world monkey homologues of human poliovirus receptor CD155 . J. Virol., Jul 2008; 82: 7167 - 7179.

209413728

NP_001129240.1

NM_001135768.1

P15151

45,303 Da

Adaptive Immunity Signaling Pathway (366160); Adherens Junctions Interactions Pathway (119533); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Junction Organization Pathway (160966); Cell-cell Junction Organization Pathway (119532); Immune System Pathway (106386); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (106413); Nectin Adhesion Pathway (138054); Nectin/Necl Trans Heterodimerization Pathway (119534)

The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

PVR: Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. Serves as a receptor for poliovirus attachment to target cells. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport. Belongs to the nectin family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Immunoglobulin superfamily; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc. Chromosomal Location of Human Ortholog: 19q13.2. Cellular Component: extracellular space; cell surface; focal adhesion; cytoplasm; integral to membrane; plasma membrane. Molecular Function: viral receptor activity; protein binding; receptor activity; cell adhesion molecule binding. Biological Process: intercellular junction assembly and maintenance; regulation of immune response; cell migration; cell-cell adhesion; entry of virus into host cell; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; response to virus; susceptibility to natural killer cell mediated cytotoxicity; positive regulation of natural killer cell mediated cytotoxicity

0.02 mg

220 USD

0.1 mg

540

0.5 mg

1915