Human CD16a Recombinant (FcgR3a) | MyBioSource MBS553215 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Human CD16a Recombinant (FcgR3a)

catalog :

MBS553215

quantity :

0.02 mg

price :

220 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS553215

products type :

Recombinant Protein

products full name :

Human CD16a Recombinant (FcgR3a)

products short name :

CD16a (FcgR3a)

products name syn :

Human CD16a Recombinant (FcgrR3a) ; cd16; cd-16; fcgr3a

other names :

low affinity immunoglobulin gamma Fc region receptor III-A isoform a; Low affinity immunoglobulin gamma Fc region receptor III-A; CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2

products gene name :

CD16

other gene names :

FCGR3A; CD16A; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa

uniprot entry name :

FCG3A_HUMAN

host :

HEk293 Cells

sequence length :

290

purity :

>95%, as determined by SDS-PAGE and HPLC

form :

Recombinant CD16a is supplied as a 0.2 um filtered PBS solution, pH7.2.

storage stability :

Recombinant CD16a, as supplied, can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C to -70 degree C for twelve months. Avoid repeated freeze/thaw cycles.

other info1 :

Domain: Ig-like C2. Host Note: Optimized DNA sequence encoding Human extracellular domain of human CD16a including a C-terminal 11His tag was expressed in HEK293 cells.

other info2 :

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Biologica Activity: Recombinant CD16a activity was measured by the ability to bind human IgG with an affinity constant of 180nM. Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. Molecular Weight Note: Recombinant CD16a is a monomer protein consisting of 203 amino acid residue subunits, due to glycosylation migrates as an approximately 50 kDa protein on SDS-PAGE.

products categories :

Receptors CD

products description :

Human CD16 is encoded by two genes, Fc RIIIA and Fc RIIIB. Fc RIIIa is a transmembranous isoform of the receptor expressed on the surface of natural killer (NK) cells, macrophages, a subset of monocytes and of T cells in association with dimers of the -chain of Fc RI and/or -chain of T-cell receptors. Fc RIIIb is a glycosylphosphatidylinositol -anchored isoform restricted to neutrophils. Fc RIII plays a significant role in the clearance of immune complexes, antibody- dependent cellular cytotoxicity of K/NK cells,phagocytosis and antigen presentation.

products references :

affinity and kinetic analysis of fc receptor iiia ( CD16a ) binding to igg ligands . J. Biol. Chem., Mar 2007; 282: 6210 - 6221. phagocytosis stimulates mobilization and shedding of intracellular CD16a in human monocytes and macrophages: inhibition by hiv-1 infection . J. Leukoc. Biol., Feb 2006; 79: 294 - 302.

ncbi gi num :

50726979

ncbi acc num :

NP_000560.5

ncbi gb acc num :

NM_000569.6

uniprot acc num :

P08637

ncbi mol weight :

29,089 Da

ncbi pathways :

Adaptive Immunity Signaling Pathway (366160); Fc Gamma R-mediated Phagocytosis Pathway (114228); Fc Gamma R-mediated Phagocytosis Pathway (106590); Immune System Pathway (106386); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (106413); Leishmaniasis Pathway (144181); Leishmaniasis Pathway (144028); Natural Killer Cell Mediated Cytotoxicity Pathway (83079); Natural Killer Cell Mediated Cytotoxicity Pathway (490); Osteoclast Differentiation Pathway (193147)

ncbi summary :

This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

FCGR3A: Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q23. Cellular Component: integral to membrane; plasma membrane; external side of plasma membrane. Molecular Function: IgG binding. Biological Process: regulation of immune response; innate immune response; immune response. Disease: Immunodeficiency 20

size1 :

0.02 mg

price1 :

220 USD

size2 :

0.1 mg

price2 :

540

size3 :

0.5 mg

price3 :

1915

more info or order :

MyBioSource product webpage