protein

Mouse Interleukin-1 beta Recombinant

MBS553209

0.002 mg

145 USD

Recombinant Protein

Mouse Interleukin-1 beta Recombinant

Interleukin-1 beta

interleukin-1 beta; hbgf; heparin binding; il1; il-1beta; il1b; il-1b

interleukin-1 beta; Interleukin-1 beta

IL-1

Il1b; IL-1 beta

IL1B_MOUSE

E Coli

269

MATVPELNCE MPPFDSDEND LFFEVDGPQK MKGCFQTFDL GCPDESIQLQ ISQQHINKSF RQAVSLIVAV EKLWQLPVSF PWTFQDEDMS TFFSFIFEEE PILCDSWDDD DNLLVCDVPI RQLHYRLRDE QQKSLVLSDP YELKALHLNG QNINQQVIFS MSFVQGEPSN DKIPVALGLK GKNLYLSCVM KDGTPTLQLE SVDPKQYPKK KMEKRFVFNK IEVKSKVEFE SAEFPNWYIS TSQAEHKPVF LGNNSGQDII DFTMESVSS

>98%, as determined by SDS-PAGE and HPLC

Mouse IL-1 beta was lyophilized from a 0.2 um filtered solution in PBS, pH 7.

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Domain: IL1. Host Note: Optimized DNA sequence encoding Mouse Interleukin-1 beta mature chain was expressed in Escherichia Coli.

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Molecular Weight Note: Mature Mouse IL-1 beta, is generated by the proteolytic removal of the signal peptide. and propeptide.The molecule has a calculated molecular mass of approximately 17 kDa. Recombinant IL-1 beta is a disulfide-linked monomer protein consisting of two 153 amino acid residue subunits. Recombinant IL-1 beta migrates as an approximately 17 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

Cytokines

Monocytes are the main source of secreted IL1. They express predominantly IL1- beta while human keratinocytes express large amounts of IL1-alpha. Murine macrophages display a transition from IL1-beta to IL1-alpha production during maturation of monocytes into inflammatory macrophages.There are two functionally almost equivalent forms of IL1, IL1-alpha and IL1-beta that are encoded by two different genes. IL1-beta is the predominant form in humans while it is IL1-alpha in mice. Both forms of IL1 bind to the same receptor and therefore also show similar if not identical biological activities. The IL1-beta but not the IL1-alpha precursor must be processed before it can bind to the receptor. Both forms of IL1 bind to the same receptor and therefore also show similar if not identical biological activities. The receptor isolated from T-cells is expressed predominantly on T-cells and cells of mesenchymal origin. It binds both types of IL1 with equal affinity. This type is called also Type 1 receptor. It has been designated CD121a. The Type 2 receptor has been designated CD121b. It is isolated from B-cells, granulocytes, and macrophages. It is expressed predominantly on B-cells and cells of the myelomonocytic lineage and is encoded by a separate gene.

il-1 in aqueous humor induces fgf-2 synthesis in rabbit corneal endothelium through pi 3-kinase and p38 . Invest. Ophthalmol. Vis. Sci., Sep 2009; 10.1167/iovs.09-4240. a high resolution nmr-based model for the structure of a scfv-il-1 complex: potential for nmr as a key tool in therapeutic antibody design and development . J. Biol. Chem., Sep 2009; 10.1074/jbc.M109.025304. pro-interleukin (il) -1 shares a core region of stability as compared with mature il-1 while maintaining a distinctly different configurational landscape: a comparative hydrogen/deuterium exchange mass spectrometry study . J. Biol. Chem., Sep 2009; 284: 26137 - 26148. cholesterol-dependent cytolysins induce rapid release of mature il-1 from murine macrophages in a nlrp3 inflammasome and cathepsin b-dependent manner . J. Leukoc. Biol., Aug 2009; 10.1189/jlb.0309164. pyrin critical to macrophage il-1 response to francisella challenge . J. Immunol., Jun 2009; 182: 7982 - 7989.

6680415

NP_032387.1

NM_008361.3

P10749

30,931 Da

African Trypanosomiasis Pathway (194387); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (83294); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167330); Amoebiasis Pathway (167191); Apoptosis Pathway (83257); Apoptosis Pathway (470); Chagas Disease (American Trypanosomiasis) Pathway (147810); Chagas Disease (American Trypanosomiasis) Pathway (147795)

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. The encoded protein plays a role in thymocyte proliferation and is involved in the inflammatory response. [provided by RefSeq, Aug 2015]

IL1B: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Monomer. Belongs to the IL-1 family. Protein type: Cytokine. Cellular Component: extracellular space; cell; extracellular region; intracellular; cytosol; vesicle; secretory granule. Molecular Function: protein domain specific binding; interleukin-1 receptor binding; cytokine activity; receptor binding. Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; negative regulation of MAP kinase activity; positive regulation of JNK activity; positive regulation of nitric oxide biosynthetic process; negative regulation of glutamate secretion; activation of MAPK activity; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of interleukin-2 biosynthetic process; germ cell programmed cell death; negative regulation of insulin receptor signaling pathway; positive regulation of glial cell differentiation; positive regulation of NF-kappaB import into nucleus; positive regulation of lipid catabolic process; response to lipopolysaccharide; fever; positive regulation of membrane protein ectodomain proteolysis; response to carbohydrate stimulus; activation of NF-kappaB transcription factor; elevation of cytosolic calcium ion concentration; positive regulation of phagocytosis; positive regulation of T cell proliferation; positive regulation of astrocyte differentiation; neutrophil chemotaxis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of heterotypic cell-cell adhesion; positive regulation of mitosis; positive regulation of interleukin-6 production; interleukin-1 beta production; social behavior; negative regulation of neuron differentiation; positive regulation of angiogenesis; positive regulation of cell division; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of lipid metabolic process; leukocyte migration; sequestering of triacylglycerol; positive regulation of interleukin-6 biosynthetic process; positive regulation of JNK cascade; negative regulation of transcription from RNA polymerase II promoter; positive regulation of stress-activated MAPK cascade; negative regulation of neurogenesis; positive regulation of interleukin-8 production; negative regulation of cell proliferation; negative regulation of lipid catabolic process; hyaluronan biosynthetic process; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; inflammatory response; regulation of I-kappaB kinase/NF-kappaB cascade; aging; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; positive regulation of immature T cell proliferation in the thymus; response to ATP; memory; positive regulation of interferon-gamma production; positive regulation of chemokine biosynthetic process; positive regulation of prostaglandin secretion; positive regulation of fever; immune response; positive regulation of protein amino acid phosphorylation; regulation of insulin secretion

0.002 mg

145 USD

0.01 mg

220