protein

Human Thrombospondin-1 Recombinant

MBS553204

0.002 mg

160 USD

Recombinant Protein

Human Thrombospondin-1 Recombinant

Thrombospondin-1

thrmbospondin; THBS1; tsp

thrombospondin-1; Thrombospondin-1

TSP-1

THBS1; TSP; TSP1

TSP1_HUMAN

Insect Cells

1170

MGLAWGLGVL FLMHVCGTNR IPESGGDNSV FDIFELTGAA RKGSGRRLVK GPDPSSPAFR IEDANLIPPV PDDKFQDLVD AVRAEKGFLL LASLRQMKKT RGTLLALERK DHSGQVFSVV SNGKAGTLDL SLTVQGKQHV VSVEEALLAT GQWKSITLFV QEDRAQLYID CEKMENAELD VPIQSVFTRD LASIARLRIA KGGVNDNFQG VLQNVRFVFG TTPEDILRNK GCSSSTSVLL TLDNNVVNGS SPAIRTNYIG HKTKDLQAIC GISCDELSSM VLELRGLRTI VTTLQDSIRK VTEENKELAN ELRRPPLCYH NGVQYRNNEE WTVDSCTECH CQNSVTICKK VSCPIMPCSN ATVPDGECCP RCWPSDSADD GWSPWSEWTS CSTSCGNGIQ QRGRSCDSLN NRCEGSSVQT RTCHIQECDK RFKQDGGWSH WSPWSSCSVT CGDGVITRIR LCNSPSPQMN GKPCEGEARE TKACKKDACP INGGWGPWSP WDICSVTCGG GVQKRSRLCN NPTPQFGGKD CVGDVTENQI CNKQDCPIDG CLSNPCFAGV KCTSYPDGSW KCGACPPGYS GNGIQCTDVD ECKEVPDACF NHNGEHRCEN TDPGYNCLPC PPRFTGSQPF GQGVEHATAN KQVCKPRNPC TDGTHDCNKN AKCNYLGHYS DPMYRCECKP GYAGNGIICG EDTDLDGWPN ENLVCVANAT YHCKKDNCPN LPNSGQEDYD KDGIGDACDD DDDNDKIPDD RDNCPFHYNP AQYDYDRDDV GDRCDNCPYN HNPDQADTDN NGEGDACAAD IDGDGILNER DNCQYVYNVD QRDTDMDGVG DQCDNCPLEH NPDQLDSDSD RIGDTCDNNQ DIDEDGHQNN LDNCPYVPNA NQADHDKDGK GDACDHDDDN DGIPDDKDNC RLVPNPDQKD SDGDGRGDAC KDDFDHDSVP DIDDICPENV DISETDFRRF QMIPLDPKGT SQNDPNWVVR HQGKELVQTV NCDPGLAVGY DEFNAVDFSG TFFINTERDD DYAGFVFGYQ SSSRFYVVMW KQVTQSYWDT NPTRAQGYSG LSVKVVNSTT GPGEHLRNAL WHTGNTPGQV RTLWHDPRHI GWKDFTAYRW RLSHRPKTGF IRVVMYEGKK IMADSGPIYD KTYAGGRLGL FVFSQEMVFF SDLKYECRDP

>90%, as determined by SDS-PAGE and HPLC

Recombinant Thrombospondin-1 is presented as liquid formulation of 0.3M NaCl, 0.02M NaH2PO^4 pH 6.0. Appearance: Sterile solution.

This cytokine should be stored in working aliquots at 2 degree - 8 degree C for one year. Avoid freezing.

Domain: EGF. Host Note: Optimized DNA sequence encoding Human Thrombospondin mature chain was expressed in insect cells.

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Molecular Weight Note: Native human Thrombospondin, generated by the proteolytic removal of the signal peptide and propeptide, and has a calculated molecular mass of approximately 128 kDa. Recombinant human TSP is a disulfide-linked monomer protein consisting of 1152 amino acid residue subunits, and migrates due to glycosylation as an approximately 140 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

Cytokines

TSP is an adhesive protein that can bind to many different cell types, including hematopoietic progenitor cells. It possesses a number of functional domains that serve as receptor recognizing regions. The amino-terminal heparin binding domain interacts with heparin, other glycosaminoglycans and glycolipids and likely recognizes specific cell surface proteoglycans. The central disulfide cross- linked region is responsible for high affinity binding to one of the CD antigens, CD36. TSP has been demonstrated to bind to integrin-alpha-V, integrin-beta-3 and integrin-alpha-2b, integrin- beta-3. The carboxy-terminal region of TSP also contains at least one binding epitope for a cell receptor. TSP can interact with fibrinogen and fibronectin. TSP appears to be involved intimately in the regulation of cellular proliferation. It is expressed as an early response gene following treatment of cells with various mitogens and cytokines. It modulates the progression of cells through the Cell cycle. It augments the proliferative response to EGF. TSP inhibits endothelial cell chemotactic response to bFGF and reduces their proliferative response to serum and bFGF.

regulation of Thrombospondin-1 by natural and synthetic progestins in human breast cancer cells . Endocr. Relat. Cancer, Sep 2009; 16: 809 - 817. sj gren s syndrome-like ocular surface disease in Thrombospondin-1 deficient mice . Am. J. Pathol., Sep 2009; 175: 1136 - 1147. interactions among stalk modules of Thrombospondin-1 . J. Biol. Chem., Aug 2009; 10.1074/jbc.M109.035089. interactions among the epidermal growth factor-like modules of Thrombospondin-1 . J. Biol. Chem., Aug 2009; 284: 22206 - 22212. Thrombospondin-1 is a transcriptional repression target of prmt6 . J. Biol. Chem., Aug 2009; 284: 21338 - 21346.

40317626

NP_003237.2

NM_003246.2

P07996

129,383 Da

Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Formation Of Platelet Plug Pathway (106029); Hemostasis Pathway (106028); Inflammatory Response Pathway (198766)

The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq, Jul 2008]

THBS1: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Ligand for CD36 mediating antiangiogenic properties. Belongs to the thrombospondin family. Protein type: Inhibitor; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 15q15. Cellular Component: extracellular matrix; extracellular space; cell surface; sarcoplasmic reticulum; endoplasmic reticulum; endoplasmic reticulum lumen; fibrinogen complex; extracellular region; secretory granule; external side of plasma membrane. Molecular Function: integrin binding; heparin binding; identical protein binding; proteoglycan binding; protein binding; fibroblast growth factor binding; phosphatidylserine binding; transforming growth factor beta binding; fibronectin binding; low-density lipoprotein binding; laminin binding; calcium ion binding; glycoprotein binding. Biological Process: extracellular matrix organization and biogenesis; activation of MAPK activity; response to magnesium ion; negative regulation of fibrinolysis; response to glucose stimulus; cell cycle arrest; cell adhesion; positive regulation of macrophage activation; response to drug; platelet activation; negative regulation of interleukin-12 production; positive regulation of chemotaxis; positive regulation of blood vessel endothelial cell migration; response to testosterone stimulus; negative regulation of cell-matrix adhesion; negative regulation of blood vessel endothelial cell migration; response to unfolded protein; positive regulation of angiogenesis; response to mechanical stimulus; negative regulation of endothelial cell proliferation; peptide cross-linking; regulation of cGMP metabolic process; response to progesterone stimulus; response to calcium ion; negative regulation of apoptosis; positive regulation of blood coagulation; positive regulation of translation; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of antigen processing and presentation of peptide or polysaccharide antigen via MHC class II; negative regulation of caspase activity; behavioral response to pain; platelet degranulation; positive regulation of tumor necrosis factor biosynthetic process; protein amino acid O-linked glycosylation; positive regulation of transforming growth factor-beta1 production; chronic inflammatory response; cell migration; negative regulation of focal adhesion formation; positive regulation of transforming growth factor beta receptor signaling pathway; engulfment of apoptotic cell; post-translational protein modification; positive regulation of protein kinase B signaling cascade; negative regulation of angiogenesis; cellular protein metabolic process; negative regulation of dendritic cell antigen processing and presentation; response to hypoxia; immune response; sprouting angiogenesis; blood coagulation; positive regulation of phosphorylation; positive regulation of cell migration

0.002 mg

160 USD

0.01 mg

315