protein

Human Neuregulin 1 beta Recombinant

MBS553198

0.01 mg

145 USD

Recombinant Protein

Human Neuregulin 1 beta Recombinant

Neuregulin 1 beta

neuregulin; nrg1; nrg-1; heregulin; hrg

pro-neuregulin-1, membrane-bound isoform isoform HRG-beta1c; Pro-neuregulin-1, membrane-bound isoform; Acetylcholine receptor-inducing activity; ARIA; Breast cancer cell differentiation factor p45; Glial growth factor; Heregulin; HRG; Neu differentiation factor; Sensory and motor neuron-derived factor

NRG1

NRG1; GGF; HGL; HRGA; NDF; SMDF; Pro-NRG1; ARIA; HRG

NRG1_HUMAN

E Coli

607

MSERKEGRGK GKGKKKERGS GKKPESAAGS QSPALPPRLK EMKSQESAAG SKLVLRCETS SEYSSLRFKW FKNGNELNRK NKPQNIKIQK KPGKSELRIN KASLADSGEY MCKVISKLGN DSASANITIV ESNEIITGMP ASTEGAYVSS ESPIRISVST EGANTSSSTS TSTTGTSHLV KCAEKEKTFC VNGGECFMVK DLSNPSRYLC KCPNEFTGDR CQNYVMASFY KHLGIEFMEQ KRVLTITGIC IALLVVGIMC VVAYCKTKKQ RKKLHDRLRQ SLRSERNNMM NIANGPHHPN

>96%, as determined by SDS-PAGE and HPLC

Recombinant Neuregulin-1 was lyophilized from a 0.2 um filtered 10mM PB, pH 7.0,. containing 1% mannitol, 0.2% HSA.

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Domain: EGF. Host Note: Optimized DNA sequence encoding Human Neuregulin-1 beta EGF domain was expressed in Escherichia Coli. Biological Activity:: The ED(50) was determined by the dose-dependent proliferation of human MCF-7 cells was found to be <0.3ng/ml, corresponding to a specific activity of 2x 10^4 Units/mg.

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Molecular Weight Note: Recombinant human Neuregulin-1 beta EGF domain is a disulfide-linked monomeric protein consisting of 62 amino acid residue subunits, and migrates as an approximately 7 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

Cytokines

Neuregulins (NDF, heregulin, GGF ARIA, or SMDF) are EGF- like growth and differentiation factors that signal through tyrosine kinase receptors of the ErbB family.The ErbB2 and ErbB4 receptors cooperate in transmission of neuregulin-1 signals in the heart, whereas ErbB2 and ErbB3 cooperate in neural crest cells.

the tyrosine phosphatase shp2 (ptpn11) directs Neuregulin-1 /erbb signaling throughout schwann cell development . PNAS, Sep 2009; 10.1073/pnas.0904336106. Neuregulin-1 is associated with disease severity and adverse outcomes in chronic heart failure . Circulation, Jul 2009; 120: 310 - 317. the relationship among neuregulin 1 stimulated phosphorylation of akt, psychosis proneness, and habituation of arousal in nonclinical individuals . Schizophr Bull, Jun 2009; 10.1093/schbul/sbp063. sensory axon-derived Neuregulin-1 is required for axoglial signaling and normal sensory function but not for long-term axon maintenance . J. Neurosci., Jun 2009; 29: 7667 - 7678. downregulation of neuregulin 1 in retinal ganglion cells in experimental glaucoma . Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 2075.

236461509

NP_001153467.1

NM_001159995.1

Q02297

70,392 Da

ErbB Signaling Pathway (198844); ErbB Signaling Pathway (83049); ErbB Signaling Pathway (458); Glypican 1 Network Pathway (138010); MicroRNAs In Cardiomyocyte Hypertrophy Pathway (198784)

The protein encoded by this gene is a membrane glycoprotein that that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. An extraordinary variety of different isoforms are produced from this gene through alternative promoter usage and splicing. These isoforms are expressed in a tissue-specific manner and differ significantly in their structure, and are classified as types I, II, III, IV, V and VI. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD). [provided by RefSeq, Jun 2014]

NRG1: Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. The cytoplasmic domain interacts with the LIM domain region of LIMK1. Interacts with ERBB3 and ERBB4. Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells; in the nervous system it is expressed in spinal cord and brain. Also detected in adult heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific. Belongs to the neuregulin family. 10 isoforms of the human protein are produced by alternative splicing. Protein type: Cell development/differentiation; Cytokine; Ligand, receptor tyrosine kinase; Membrane protein, integral; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 8p12. Cellular Component: extracellular space; membrane; axon; integral to plasma membrane; cytoplasm; apical plasma membrane; extracellular region; neuromuscular junction; nucleus. Molecular Function: ErbB-2 class receptor binding; protein binding; transmembrane receptor protein tyrosine kinase activator activity; growth factor activity; ErbB-3 class receptor binding; transcription cofactor activity; cytokine activity; protein tyrosine kinase activator activity; receptor tyrosine kinase binding; receptor binding. Biological Process: regulation of protein heterodimerization activity; positive regulation of cell adhesion; transmembrane receptor protein tyrosine kinase activation (dimerization); cellular protein complex disassembly; nerve growth factor receptor signaling pathway; wound healing; neural crest cell development; cell morphogenesis; ventricular cardiac muscle cell differentiation; locomotory behavior; positive regulation of striated muscle cell differentiation; cardiac muscle cell differentiation; synaptogenesis; mammary gland development; cell communication; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; nervous system development; cell migration; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; neurotransmitter receptor metabolic process; regulation of protein homodimerization activity; neuron fate commitment; MAPKKK cascade; positive regulation of cell growth; peripheral nervous system development; cell proliferation; positive regulation of protein kinase B signaling cascade; embryonic development; glial cell fate commitment; innate immune response; negative regulation of secretion; positive regulation of Ras protein signal transduction; negative regulation of protein catabolic process; negative regulation of transcription, DNA-dependent; transmembrane receptor protein tyrosine kinase signaling pathway. Disease: Schizophrenia 6

0.01 mg

145 USD

0.05 mg

220