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protein

Human Stem Cell Factor Recombinant

MBS553194

0.01 mg

220 USD

MBS553194

Recombinant Protein

Human Stem Cell Factor Recombinant

Stem Cell Factor

Kitlg; scf; stem cell factor; kit ligand

kit ligand isoform b; Kit ligand; Mast cell growth factor; MGF; Stem cell factor; SCF; c-Kit ligand

SCF

KITLG; MGF; SCF; MGF; SCF; sKITLG

SCF_HUMAN

E Coli

273

MKKTQTWILT CIYLQLLLFN PLVKTEGICR NRVTNNVKDV TKLVANLPKD YMITLKYVPG MDVLPSHCWI SEMVVQLSDS LTDLLDKFSN ISEGLSNYSI IDKLVNIVDD LVECVKENSS KDLKKSFKSP EPRLFTPEEF FRIFNRSIDA FKDFVVASET SDCVVSSTLS PEKDSRVSVT KPFMLPPVAA SSLRNDSSSS NRKAKNPPGD SSLHWAAMAL PALFSLIIGF AFGALYWKKR QPSLTRAVEN IQINEEDNEI SMLQEKEREF QEV

>97%, as determined by SDS-PAGE and HPLC

Recombinant SCF was lyophilized from a 0.2 um filtered solution inPBS pH 7.0.

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Domain: four helix cytokine core. Host Note: Optimized DNA sequence encoding Human SCF mature chain was expressed in Escherichia Coli.

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Molecular Weight Note: Native human Stem Cell Factor is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 18 kDa. Recombinant SCF is a disulfide-linked monomer protein consisting of 165 amino acid residue subunits, and migrates as an approximately 18 kDa protein under non-reducing. and reducing conditions in SDS-PAGE.

Cytokines

SCF is a stromal cell-derived cytokine synthesized by fibroblasts and other cell types. SCF promotes proliferation and early differentiation of cells at the level of multipotential stem cells. It has been suggested that SCF is essential for optimal production of various hematopoietic lineages, mainly because of its ability to prevent apoptosis when it co-stimulates with other cytokines. The receptor for SCF, designated SCFR (CD117), is the oncogene designated as KIT. The biological activities of SCF are synergised considerably by colony stimulating factors GM-CSF and G-CSF, and also by IL7, Epo and some other growth and differentiation factors. In combination with IL7 SCF stimulates the proliferation of pre-B-cells. SCF is also a potent chemoattractant for cells (see also: Chemotaxis), for example, mast cells, expressing the kit receptor. One response to SCF in these cells is a characteristic rearrangement of the actin filaments of the cytoskeleton.

pollen-specific skp1-like proteins are components of functional SCF complexes and essential for lily pollen tube elongation . Plant Cell Physiol., Aug 2009; 50: 1558 - 1572. SCF induces -globin gene expression by regulating downstream transcription factor coup-tfii . Blood, Jul 2009; 114: 187 - 194. jfk, a kelch domain-containing f-box protein, links the SCF complex to p53 regulation . PNAS, Jun 2009; 106: 10195 - 10200. the extracellular signal-regulated kinase mitogen-activated protein kinase pathway phosphorylates and targets cdc25a for SCF -trcp-dependent degradation for cell cycle arrest . Mol. Biol. Cell, Apr 2009; 20: 2186 - 2195. mmp9 and SCF protect from apoptosis in acute kidney injury . J. Am. Soc. Nephrol., Apr 2009; 20: 787 - 797.

4505175

NP_000890.1

NM_000899.4

P21583

30,899 Da

C-MYB Transcription Factor Network Pathway 138073!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Hematopoietic Cell Lineage Pathway 83078!!Hematopoietic Cell Lineage Pathway 489!!Kit Receptor Signaling Pathway 198826!!Melanogenesis Pathway 83092!!Melanogenesis Pathway 504!!Pathways In Cancer 83105!!Signaling Events Mediated By Stem Cell Factor Receptor (c-Kit) Pathway 138068

Function: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. Subunit structure: Homodimer, non-covalently linked . Probable. Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein Ref.9. Isoform 2: Cell membrane; Single-pass type I membrane protein . By similarity. Cytoplasm cytoskeleton . By similarity Ref.9. Soluble KIT ligand: Secreted Ref.9. Developmental stage: Acts in the early stages of hematopoiesis. Post-translational modification: A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97. Ref.9A soluble form exists as a cleavage product of the extracellular domain. Polymorphism: Genetic variants in KITLG define the skin/hair/eye pigmentation variation locus 7 (SHEP7) [. MIM:611664]. Hair, eye and skin pigmentation are among the most visible examples of human phenotypic variation, with a broad normal range that is subject to substantial geographic stratification. In the case of skin, individuals tend to have lighter pigmentation with increasing distance from the equator. By contrast, the majority of variation in human eye and hair color is found among individuals of European ancestry, with most other human populations fixed for brown eyes and black hair. Involvement in disease: Familial progressive hyperpigmentation (FPH) [MIM:145250]: A disorder characterized by hyperpigmented patches in the skin, present in early infancy and increasing in size and number with age.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.18. Sequence similarities: Belongs to the SCF family.

0.01 mg

220 USD