catalog number :
MBS553191
products type :
Recombinant Protein
products full name :
Human HIV-1 gp120 Recombinant
products short name :
HIV-1 gp120
products name syn :
hiv1; gp120
products gene name :
HIV-1 gp120
specificity :
immunoreative with sera of infected HIV individuals.
purity :
>90%, as determined by SDS-PAGE.
form :
Lyophilized from 20mM PBS pH-7.8, 20mM NaCl 0.5M, 1mM DTT & 8M urea.
storage stability :
Stable for at least 1 year from date of receipt at -18 deg. C . Avoid repeated freeze/thaw cycles.
app notes :
HIV1 gp120 antigen is suitable for ELISA and Western blots, excellent antigen for detection of HIV with minimal specificity problems.
other info1 :
Host Note: DNA sequence encoding immunodominant fragment of HIV-1 gp120 was expressed in Escherichia Coli. Presentation:: 0.2um filtered 50mM Tris pH 8.0 NaCl 60mM, 10mM Glutahione, 0.25% Sarkosyl, 50% Glycerol. Endotoxin:: Less than 01EU/ug as determined by LAL method.
other info2 :
Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Molecular Weight Note: Recombinant HIV-1 gp120 encodes the immunodominant sequences of the HIV surface Glycoprotein 120. Recombinant HIV-1 Glycoprotein 120 contains an N terminal GST purification tag and migrates as an approximately 33 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
products categories :
Viral Antigens
products description :
Recombinant HIV-1 Glycoprotein gp120 contains a GST purification tag and migrates as an approximately 35 kDa protein under reducing condition in SDS-PAGE.
products references :
structure-function analysis of HIV-1 gp120 amino acid mutations associated with resistance to the ccr5 co-receptor antagonist vicriviroc . J. Virol., Sep 2009; 10.1128/JVI.01351-09. HIV-1 gp120 -induced migration of dendritic cells is regulated by a novel kinase cascade involving pyk2, p38 map kinase and lsp1 . Blood, Aug 2009; 10.1182/blood-2009-02-206342. x4 HIV-1 gp120 down-modulates expression and immunogenicity of co-delivered antigens . J. Virol., Aug 2009; 10.1128/JVI.00394-09. llama antibody fragments with cross-subtype human immunodeficiency virus type 1 (hiv-1) -neutralizing properties and high affinity for HIV-1 gp120 . J. Virol., Dec 2008; 82: 12069 - 12081. cannabinoids inhibit HIV-1 gp120 -mediated insults in brain microvascular endothelial cells . J. Immunol., Nov 2008; 181: 6406 - 6416.
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)