MyBioSource

protein

Mouse Stem Cell Factor Recombinant

MBS553178

0.01 mg

430 USD

MBS553178

Recombinant Protein

Mouse Stem Cell Factor Recombinant

Stem Cell Factor

Mouse Stem cell Factor; kit ligand; kitlg; mgf; stem cell factor; scf

kit ligand; Kit ligand; Hematopoietic growth factor KL; Mast cell growth factor; MGF; Steel factor; Stem cell factor; SCF; c-Kit ligand

SCF

Kitlg; Kitl; Mgf; Sl; Slf; MGF; SCF; sKITLG

SCF_MOUSE

Insect Cells

273

MKKTQTWIIT CIYLQLLLFN PLVKTKEICG NPVTDNVKDI TKLVANLPND YMITLNYVAG MDVLPSHCWL RDMVIQLSLS LTTLLDKFSN ISEGLSNYSI IDKLGKIVDD LVLCMEENAP KNIKESPKRP ETRSFTPEEF FSIFNRSIDA FKDFMVASDT SDCVLSSTLG PEKDSRVSVT KPFMLPPVAA SSLRNDSSSS NRKAAKAPED SGLQWTAMAL PALISLVIGF AFGALYWKKK QSSLTRAVEN IQINEEDNEI SMLQQKEREF QEV

>95%, as determined by SDS-PAGE and HPLC

Recombinant Stem cell factor was lyophilized from a 0.2 um filtered solution in 20mM Tris, 5% trehalose, pH 7.5.

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Domain: Four helix core. Host Note: Optimized DNA sequence encoding Mouse SCF mature chain was expressed in Insect cells.

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Molecular Weight Note: Native Mouse Stem Cell Factor is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 18 kDa. Recombinant SCF is a disulfide-linked monomer protein consisting of 165 amino acid residue subunits, and due to glycosylation migrates as an approximately 23 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

Cytokines

SCF is a stromal cell- derived cytokine synthesized by fibroblasts and other cell types. SCF promotes proliferation and early differentiation of cells at the level of multipotential stem cells. It has been suggested that SCF is essential for optimal production of various hematopoietic lineages, mainly because of its ability to prevent apoptosis when it co- stimulates with other cytokines. The receptor for SCF, designated SCFR (CD117), is the oncogene designated as KIT. The biological activities of SCF are synergised considerably by colony stimulating factors GM-CSF and G-CSF, and also by IL7, Epo and some other growth and differentiation factors. In combination with IL7 SCF stimulates the proliferation of pre-B-cells. SCF is also a potent chemoattractant for cells (see also: Chemotaxis), for example, mast cells, expressing the kit receptor. One response to SCF in these cells is a characteristic rearrangement of the actin filaments of the cytoskeleton.

cardiomyocyte-specific overexpression of human Stem Cell Factor improves cardiac function and survival after myocardial infarction in mice . Circulation, Sep 2009; 120: 1065 - 1074. Stem Cell Factor and interleukin-2/15 combine to enhance mapk-mediated proliferation of human natural killer cells . Blood, Mar 2009; 113: 2706 - 2714. human Stem Cell Factor (scf) is a heparin-binding cytokine . J. Biochem., Mar 2009; 145: 275 - 278. role of Stem Cell Factor and bone marrow-derived fibroblasts in airway remodeling . Am. J. Pathol., Feb 2009; 174: 390 - 400. Stem Cell Factor signaling collaborates in the development of fip1l1/pdgfr induced chronic eosinophilic leukemia in a murine model . Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 470.

7305267

NP_038626.1

NM_013598.2

P20826

30,645 Da

Cytokine-cytokine Receptor Interaction Pathway 83248!!Cytokine-cytokine Receptor Interaction Pathway 460!!Hematopoietic Cell Lineage Pathway 83275!!Hematopoietic Cell Lineage Pathway 489!!Kit Receptor Signaling Pathway 198424!!Melanogenesis Pathway 83289!!Melanogenesis Pathway 504!!Pathways In Cancer 83298

Function: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. Subunit structure: Homodimer, non-covalently linked . Probable. Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. Ref.14. Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein . By similarity. Isoform 2: Cell membrane; Single-pass type I membrane protein . By similarity. Cytoplasm cytoskeleton . By similarity. Soluble KIT ligand: Secreted . By similarity. Developmental stage: Acts in the early stages of hematopoiesis. Post-translational modification: A soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain. Sequence similarities: Belongs to the SCF family.

0.01 mg

430 USD