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protein

Human Interleukin-13 Recombinant

MBS553168

0.01 mg

220 USD

MBS553168

Recombinant Protein

Human Interleukin-13 Recombinant

Interleukin-13

il13; il-13; nc30; interleukin-13

interleukin-13; Interleukin-13; N/A

IL-13

IL13; NC30; IL-13

IL13_HUMAN

E Coli

146

MALLLTTVIA LTCLGGFASP GPVPPSTALR ELIEELVNIT QNQKAPLCNG SMVWSINLTA GMYCAALESL INVSGCSAIE KTQRMLSGFC PHKVSAGQFS SLHVRDTKIE VAQFVKDLLL HLKKLFREGR FN

>95%, as determined by SDS-PAGE and HPLC

Recombinant Interleukin-13 was lyophilized from a 0.2um filtered concentrated (1mg/ml) solution in PBS, pH 7.2.

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Domain: 4 helix cytokine core. Host Note: Optimized DNA sequence encoding Human Interleukin-13 mature chain was expressed in Escherichia Coli.

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Molecular Weight Note: Native human Interleukin-13, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 13 kDa. Recombinant IL-13 is a disulfide-linked monomer protein consisting of 115 amino acid residue subunits,and migrates as an approximately 13 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

Cytokines

Mouse IL13 is produced primarily by activated Th2 cells. Human IL13 is expressed in activated T- helper cells (resembling Th0, Th1, Th2), and T-cells expressing CD8. The alpha chain of the IL13 receptor has weak binding activity for IL13. High affinity receptors are formed when the receptor alpha chains of IL13 and IL4 receptors are coexpressed. The receptors for IL13 and IL4 share a component,a common gamma subunit that is found also in the IL2 receptor.Different IL13 receptor structures have been shown to exist on various cell types and the IL13 receptor may share more than one component with IL4 receptor. IL13 down-modulates macrophage activity, reducing the production of pro-inflammatory cytokines and chemokines in response to IFN-gamma or bacterial lypopolysaccharides. IL13 enhances the production of the IL1 receptor antagonist IL1ra. IL13 also decreases the production of nitric oxide by activated macrophages, leading to a decrease in parasiticidal activity. IL13 induces differentiation of human monocytes, enhances survival time in culture, and also induces differentiation and proliferation and Isotype switching in B-cells.

IL-13 -induced oxidative stress via microglial nadph oxidase contributes to death of hippocampal neurons in vivo . J. Immunol., Oct 2009; 183: 4666 - 4674. involvement of IL-13 in tobacco smoke induced changes in the structure and function of rat intrapulmonary airways . Am. J. Respir. Cell Mol. Biol., Sep 2009; 10.1165/rcmb.2009-0117 degree C. disruption of th2 immunity results in a gender-specific expansion of IL-13 producing accessory nk cells during helminth infection . J. Immunol., Sep 2009; 183: 3906 - 3914. distinct structural requirements for interleukin-4 (il-4) and IL-13 binding to the shared IL-13 receptor facilitate cellular tuning of cytokine responsiveness . J. Biol. Chem., Sep 2009; 284: 24289 - 24296. IL-13 receptor 2 regulates the immune and functional response to nippostrongylus brasiliensis infection . J. Immunol., Aug 2009; 183: 1934 - 1939.

26787978

NP_002179.2

NM_002188.2

P35225

15,816 Da

Asthma Pathway 83120!!Asthma Pathway 532!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Cytokines And Inflammatory Response Pathway 198794!!Fc Epsilon RI Signaling Pathway 83082!!Fc Epsilon RI Signaling Pathway 493!!Glucocorticoid Receptor Regulatory Network Pathway 138014!!IL12 Signaling Mediated By STAT4 Pathway 137936!!Jak-STAT Signaling Pathway 83077

Function: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Subunit structure: Interacts with IL13RA2. Subcellular location: Secreted. Involvement in disease: Allergic rhinitis (ALRH) [MIM:607154]: A common disease with complex inheritance characterized by mucosal inflammation caused by allergen exposure.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Sequence similarities: Belongs to the IL-4/IL-13 family. Caution: It is uncertain whether Met-1 or Met-15 is the initiator. Sequence caution: The sequence AAA36107.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAA83738.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAB01681.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAC03535.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAK53823.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence CAA48824.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

0.01 mg

220 USD