Mouse Interleukin-6 Recombinant | MyBioSource MBS553163 product information

product summary

request information :

company name :

MyBioSource

product type :

protein

product name :

Mouse Interleukin-6 Recombinant

catalog :

MBS553163

quantity :

0.01 mg

price :

220 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS553163

products type :

Recombinant Protein

products full name :

Mouse Interleukin-6 Recombinant

products short name :

Interleukin-6

products name syn :

il-6; il6; interleukin-6; B-cell stimulatory factor; bsf

other names :

interleukin-6; Interleukin-6; B-cell hybridoma growth factor; Interleukin HP-1

products gene name :

IL-6

other gene names :

Il6; Il-6; IL-6

uniprot entry name :

IL6_MOUSE

host :

E Coli

sequence length :

211

sequence :

MKFLSARDFH PVAFLGLMLV TTTAFPTSQV RRGDFTEDTT PNRPVYTTSQ VGGLITHVLW EIVEMRKELC NGNSDCMNND DALAENNLKL PEIQRNDGCY QTGYNQEICL LKISSGLLEY HSYLEYMKNN LKDNKKDKAR VLQRDTETLI HIFNQEVKDL HKIVLPTPIS NALLTDKLES QKEWLRTKTI QFILKSLEEF LKVTLRSTRQ T

purity :

>97%, as determined by SDS-PAGE and HPLC

form :

Recombinant Interleukin-6 was lyophilized from a 0.2 um filtered PBS solution.

storage stability :

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

other info1 :

Domain: four helical cytokine core. Host Note: Optimized DNA sequence encoding Mouse Interleukin-6 mature chain was expressed in Escherichia Coli.

other info2 :

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Molecular Weight Note: Native Mouse Interleukin-6, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 22 kDa. Recombinant IL-6 is a disulfide-linked homodimeric protein consisting of 188 amino acid residue subunits, and migrates as an approximately 22 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

products categories :

Cytokines

products description :

IL6 is produced by many different cell types. The main sources in vivo are stimulated monocytes, fibroblasts, and endothelial cells. Macrophages, T-cells and B-lymphocytes, granulocytes, smooth muscle cells, eosinophils, chondrocytes, osteoblasts, mast cells, glial cells, and keratinocytes also produce IL6 after stimulation. IL6 is a member of a family of cytokines, which also includes LIF, CNTF, Oncostatin M, IL11, and CT- 1. All known members of the IL6 cytokine family induce hepatic expression of acute phase proteins. The IL6 receptor is expressed on T-cells, mitogen-activated B-cells, peripheral monocytes and some macrophage- and B-cell derived tumor cell types. It is not expressed in resting B-cells but in resting T- cells. The IL6 receptor is a strongly glycosylated protein of 80 kDa and a length of 449 amino acids. It has been designated CD126.

products references :

IL-6 protects against hyperoxia-induced mitochondrial damage via bcl-2 induced bak interactions with mitofusions . Am. J. Respir. Cell Mol. Biol., Oct 2009; 41: 385 - 396. long-term safety and efficacy of tocilizumab, an anti- IL-6 receptor monoclonal antibody, in monotherapy, in patients with rheumatoid arthritis (the stream study) : evidence of safety and efficacy in a 5-year extension study . Ann Rheum Dis, Oct 2009; 68: 1580 - 1584. the second bovine -galactoside-2,6-sialyltransferase (st6gal ii) : genomic organization and stimulation of its in vitro expression by IL-6 in bovine mammary epithelial cells . Glycobiology, Oct 2009; 19: 1082 - 1093. IL-6 -174g/c promoter polymorphism in acute appendicitis . Lab Med, Oct 2009; 40: 600 - 603. cell surface-bound il-1 is an upstream regulator of the senescence-associated IL-6 /il-8 cytokine network . PNAS, Sep 2009; 10.1073/pnas.0905299106.

ncbi gi num :

13624311

ncbi acc num :

NP_112445.1

ncbi gb acc num :

NM_031168.1

uniprot acc num :

P08505

ncbi mol weight :

24,384 Da

ncbi pathways :

Adipogenesis Pathway 198299!!African Trypanosomiasis Pathway 194387!!African Trypanosomiasis Pathway 194323!!Amoebiasis Pathway 167330!!Amoebiasis Pathway 167191!!Androgen Receptor Signaling Pathway 198319!!Chagas Disease (American Trypanosomiasis) Pathway 147810!!Chagas Disease (American Trypanosomiasis) Pathway 147795!!Cytokine-cytokine Receptor Interaction Pathway 83248!!Cytokine-cytokine Receptor Interaction Pathway 460

uniprot summary :

Function: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance . By similarity. Subcellular location: Secreted. Sequence similarities: Belongs to the IL-6 superfamily.

size :

0.01 mg

price :

220 USD

more info or order :

MyBioSource product webpage