MyBioSource

protein

Mouse Tumor Necrosis Factor-alpha Recombinant

MBS553156

0.02 mg

220 USD

MBS553156

Recombinant Protein

Mouse Tumor Necrosis Factor-alpha Recombinant

Tumor Necrosis Factor-alpha

tnf-alpha; tumor necrosis factor; cachetin; tnfa

tumor necrosis factor; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-aCleaved into the following 6 chains:Tumor necrosis factor, membrane form; Alternative name(s):; N-terminal fragment

TNF

Tnf; Tnfa; Tnfsf2; TNF-a; NTF; ICD1; ICD2

TNFA_MOUSE

E Coli

235

MSTESMIRDV ELAEEALPQK MGGFQNSRRC LCLSLFSFLL VAGATTLFCL LNFGVIGPQR DEKFPNGLPL ISSMAQTLTL RSSSQNSSDK PVAHVVANHQ VEEQLEWLSQ RANALLANGM DLKDNQLVVP ADGLYLVYSQ VLFKGQGCPD YVLLTHTVSR FAISYQEKVN LLSAVKSPCP KDTPEGAELK PWYEPIYLGG VFQLEKGDQL SAEVNLPKYL DFAESGQVYF GVIAL

>97%, as determined by SDS-PAGE and HPLC

Recombinant mouse TNF alpha was lyophilized from 0.2 um filtered PBS solution, pH 7.0.

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Domain: TNF. Host Note: Optimized DNA sequence encoding Mouse Tumor Necrosis Factor-alpha extracellular domain was expressed in Escherichia Coli.

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Molecular Weight Note: Native Mouse Tumor Necrosis Factor-alpha is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated mass of approximately 17kDa. Recombinant Mouse TNF is a disulfide-linked monomer protein, consisting of 157 amino acids and migrates as an approximately 17 kDa protein under reducing conditions.

Cytokines

TNF is secreted by macrophages, monocytes, neutrophils, T-cells, NK- cells following their stimulation by bacterial lipopolysaccharides. Cells expressing CD4 secrete TNF- alpha while CD8 (+) cells secrete little or no TNF- alpha. Stimulated peripheral neutrophilic granulocytes but also unstimulated cells and also a number of transformed cell lines, astrocytes, microglial cells, smooth muscle cells, and fibroblasts also secrete TNF. Human milk also contains this factor.

tak1-mediated serine/threonine phosphorylation of epidermal growth factor receptor via p38/extracellular signal-regulated kinase: nf-b-independent survival pathways in tumor necrosis factor alpha signaling . Mol. Cell. Biol., Oct 2009; 29: 5529 - 5539. tumor necrosis factor--mediated regulation of the inositol 1,4,5-trisphosphate receptor promoter . J. Biol. Chem., Oct 2009; 284: 27557 - 27566. tnf- inhibition attenuates adverse myocardial remodeling in a rat model of volume overload . Am J Physiol Heart Circ Physiol, Oct 2009; 297: H1462 - H1468. tnf inhibits apoptotic cell clearance in the lung, exacerbating acute inflammation . Am J Physiol Lung Cell Mol Physiol, Oct 2009; 297: L586 - L595. association of tnf- gene polymorphisms with systemic lupus erythematosus in taiwanese patients . Lupus, Oct 2009; 18: 974 - 979.

7305585

NP_038721.1

NM_013693.2

P06804

25,896 Da

Adipocytokine Signaling Pathway 83290!!Adipocytokine Signaling Pathway 505!!Adipogenesis Pathway 198299!!African Trypanosomiasis Pathway 194387!!African Trypanosomiasis Pathway 194323!!Allograft Rejection Pathway 83316!!Allograft Rejection Pathway 535!!Alzheimer's Disease Pathway 83294!!Alzheimer's Disease Pathway 509!!Amoebiasis Pathway 167330

Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells . By similarity. Subunit structure: Interacts with SPPL2B . By similarity. Homotrimer. Subcellular location: Cell membrane; Single-pass type II membrane protein. Tumor necrosis factor, membrane form: Membrane; Single-pass type II membrane protein . By similarity. Tumor necrosis factor, soluble form: Secreted. C-domain 1: Secreted . By similarity. C-domain 2: Secreted . By similarity. Post-translational modification: The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space . By similarity. The soluble form derives from the membrane form by proteolytic processing.The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1 . By similarity.O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid . By similarity. Sequence similarities: Belongs to the tumor necrosis factor family.

0.02 mg

220 USD