protein

Human CD40 Ligand Recombinant

MBS553154

0.01 mg

145 USD

Recombinant Protein

Human CD40 Ligand Recombinant

CD40 Ligand

cd40l; cd40 ligand; trap; cd40lg; CD154; TNFSF5

CD40 ligand; CD40 ligand; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5

CD40 Ligand

CD40LG; CD40L; TNFSF5; TRAP; CD40-L; TRAP

CD40L_HUMAN

E Coli

261

MIETYNQTSP RSAATGLPIS MKIFMYLLTV FLITQMIGSA LFAVYLHRRL DKIEDERNLH EDFVFMKTIQ RCNTGERSLS LLNCEEIKSQ FEGFVKDIML NKEETKKENS FEMQKGDQNP QIAAHVISEA SSKTTSVLQW AEKGYYTMSN NLVTLENGKQ LTVKRQGLYY IYAQVTFCSN REASSQAPFI ASLCLKSPGR FERILLRAAN THSSAKPCGQ QSIHLGGVFE LQPGASVFVN VTDPSQVSHG TGFTSFGLLK L

>95%, as determined by SDS-PAGE and HPLC

CD40 ligand was lyophilized from a 0.2 um filtered PBS solution pH 7.0.

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Domain: TNF. Host Note: Optimized DNA sequence encoding Human CD40L TNF domain was expressed in Escherichia Coli.

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Molecular Weight Note: Recombinant CD40 Ligand is a disulfide-linked monomer protein consisting of 150 amino acid residue subunits, and migrates as an approximately 16 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

Cytokines

CD40 protein is expressed on all B-lymphocytes during various stages of development, activated T- cells and monocytes, follicular dendritic cells, thymic epithelial cells, and various carcinoma cell lines. It is expressed on most mature B-cell malignancies and on some early B-cell acute lymphocytic leukemias. Human CD40 has been found to be the receptor for a protein involved in an X- linked immunodeficiency syndrome. The CD40 ligand is identical with a T-cell restricted cell surface activation antigen designated 5c8. The mouse ligand of CD40 is gp39.Based on its homology with other members of the TNF ligand protein superfamily the protein is referred to also as TNFSF5.

the cd40/ CD40 Ligand system: linking inflammation with atherothrombosis . J. Am. Coll. Cardiol., Aug 2009; 54: 669 - 677. growth inhibition of human multiple myeloma cells by an oncolytic adenovirus carrying the CD40 Ligand transgene . Clin. Cancer Res., Aug 2009; 15: 4847 - 4856. matrix metalloproteinase 9 is involved in crohn s disease-associated platelet hyperactivation through the release of soluble CD40 Ligand . Gut, Jul 2009; 58: 920 - 928. distinct roles of cd28- and CD40 Ligand -mediated costimulation in the development of protective immunity and pathology during chlamydia muridarum urogenital infection in mice . Infect. Immun., Jul 2009; 77: 3080 - 3089. phase i study of intranodal direct injection of adenovirus encoding recombinant cd40-ligand (ad-isf35) in patients with chronic lymphocytic leukemia . ASCO Meeting Abstracts, May 2009; 27: 3003.

4557433

NP_000065.1

NM_000074.2

P29965

29,274 Da

Adaptive Immunity Signaling Pathway 366160!!Allograft Rejection Pathway 83123!!Allograft Rejection Pathway 535!!Asthma Pathway 83120!!Asthma Pathway 532!!Autoimmune Thyroid Disease Pathway 83121!!Autoimmune Thyroid Disease Pathway 533!!CD40/CD40L Signaling Pathway 138061!!Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway 137993!!Calcium Signaling In The CD4+ TCR Pathway 137941

Function: Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Ref.10Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. Ref.10. Subunit structure: Homotrimer. Subcellular location: Cell membrane; Single-pass type II membrane protein. CD40 ligand, soluble form: Secreted. Tissue specificity: Specifically expressed on activated CD4+ T-lymphocytes. Post-translational modification: The soluble form derives from the membrane form by proteolytic processing.N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.Not O-glycosylated. Ref.9. Involvement in disease: X-linked immunodeficiency with hyper-IgM 1 (HIGM1) [MIM:308230]: Immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.3 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21. Sequence similarities: Belongs to the tumor necrosis factor family.

0.01 mg

145 USD