catalog number : 
MBS553147
products type : 
Recombinant Protein
products full name : 
Epstein-Barr Virus Early Antigen Recombinant
products short name : 
Epstein-Barr Virus Early Antigen
products name syn : 
ea; early antigen; ebv; epstein barr
other names : 
BMRF1; DNA polymerase processivity factor BMRF1; Early antigen protein D; EA-D; Polymerase accessory subunit
products gene name : 
EBV EA
other gene names : 
EA-D; EA-D
uniprot entry name : 
EAD_EBVB9
purity : 
>90%, as determined by SDS-PAGE.
form : 
50mM Tris-HCl pH 8, 10mM glutathione, 60mM NaCl and 50% Glycerol.
storage stability : 
This protein can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.   Avoid repeated freeze/thaw cycles.
app notes : 
Recombinant human EBV EA antigen is suitable for ELISA and Western blots, excellent antigen for detection of EBV with minimal specificity problems.
other info1 : 
Host Note: DNA sequence encoding immunodominant fragment of Epstein-Barr Early Antigen expressed in Escherichia Coli.
other info2 : 
Molecular Weight Note: Recombinant EBV EA encodes the C terminal immunodominant sequence (306-390) of the Epstein-Barr Virus Early Antigen protein D BMRF1.   Recombinant EBV EA contains an N terminal GST purification tag and migrates as an approximately 36 kDa protein under non-reducing conditions conditions in SDS-PAGE.
products categories : 
Viral Antigens
ncbi acc num : 
YP_401657.1
ncbi gb acc num : 
NC_007605.1
ncbi mol weight : 
43,374 Da
uniprot summary : 
Function: Plays an essential role in the viral lytic DNA replication by acting as the polymerase accessory subunit. Stimulates the viral DNA polymerase activity and appears to function with it as a holoenzyme. Increases the processivity of the viral polymerase, probably by acting as a sliding clamp that prevents dissociation of the polymerase from the active template. In addition, BMRF1 transcriptionally activates the early BHLF1 promoter. Ref.4 Ref.9.  Subunit structure: Homooligomerizes and adopts an oligomeric ring-shaped structure composed of 6 subunits. Forms a complex with the DNA-binding protein BALF2, the DNA polymerase subunit BALF5, and the alkaline exonuclease BGLF5. Interacts with BZLF1; this interaction may inhibit BZLF1-induced transcription of the BMRF1 promoter. Ref.4 Ref.5 Ref.6.  Subcellular location: Virion tegument. Host nucleus. Note: BMRF1 shows homogeneous, not dot-like, distribution in the replication compartments, which coincides with the newly synthesized viral DNA. Ref.7 Ref.8.  Post-translational modification: Phosphorylated by the viral BGLF4 kinase. Ref.10.  Sequence similarities: Belongs to the herpesviridae DNA polymerase accessory subunit family.