protein

Recombinant Herpes Simplex Virus-2 gD

MBS553145

0.5 mg

775 USD

Recombinant Protein

Recombinant Herpes Simplex Virus-2 gD

Herpes Simplex Virus-2 gD

Herpes Simplex Virus-2 gD Recombinant ; hsv-2; hsv2; herpes simplex; hhv

Envelope glycoprotein D; Envelope glycoprotein D

HSV-2 gD

gD; US6; gD

GD_HHV23

E Coli

393

>95%, as determined by SDS-PAGE.

Lyophilized from 25mM Tris-Hcl pH 7.2, 1mM EDTA and 50% glycerol.

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this protein can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Recombinant human Herpesvirus-2 gD antigen is suitable for ELISA and Western blots, excellent antigen for detection of HSV with minimal specificity problems.

Host Note: DNA sequence encoding immunodominant fragment of human Herpesvirus-2 Glycoprotein D was expressed in Escherichia Coli.

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Molecular Weight Note: Recombinant HSV-2 gD encodes the immunodominant sequences of the human Herpesvirus Glycoprotein D. This molecule has a calculated mass of approximately 14 kDa. Recombinant HSV2 gD contains an N terminal GST purification tag and migrates as an approximately 40 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.

Viral Antigens

Herpes simplex virus (HSV) glycoprotein D (gD) is essential for virus entry into cells, as well as for cell fusion and cell-to-cell spread. The functions of gD are best understood for virus entry, a process in which gD binds to "gD receptors," cell surface molecules that are more restricted in number than the glycosaminoglycans to which the virus initially adsorbs.

262527545

P03172.2

P03172

43,220 Da

Focal Adhesion Pathway 83067!!Focal Adhesion Pathway 478!!MAPK Signaling Pathway 83048!!MAPK Signaling Pathway 456!!Salmonella Infection Pathway 375172!!Salmonella Infection Pathway 375149

Function: Envelope glycoprotein that binds to the potential host cell entry receptors TNFRSF14/HVEM, PVRL1 and PVRL1. May trigger fusion with host membrane, by recruiting the fusion machinery composed of gB and gH/gL . By similarity. Subunit structure: Homodimer . By similarity. Interacts with host receptor TNFRSF14. Interacts with host receptor PVRL1. Interacts with host receptor PVRL2. Interacts (via profusion domain) with gB; this interaction occurs in the absence of gH/gL. Interacts (via profusion domain) with gH/gL heterodimer; this interaction occurs in the absence of gB. Associates with the gB-gH/gL-gD complex. Interacts (via C-terminus) with UL11 tegument protein . By similarity. Ref.6 Ref.7. Subcellular location: Virion membrane; Single-pass type I membrane protein . By similarity. Note: During virion morphogenesis, this protein probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs . By similarity. Sequence similarities: Belongs to the herpesviridae glycoprotein D family.

0.5 mg

775 USD