Human CD80 Recombinant (B7-1) | MyBioSource MBS553137 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Human CD80 Recombinant (B7-1)

catalog :

MBS553137

quantity :

0.05 mg

price :

220 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS553137

products type :

Recombinant Protein

products full name :

Human CD80 Recombinant (B7-1)

products short name :

CD80 (B7-1)

products name syn :

Human CD80 Recombinant (B7-1) ; CD80; B7-1

other names :

T-lymphocyte activation antigen CD80; T-lymphocyte activation antigen CD80; Activation B7-1 antigen; BB1; CTLA-4 counter-receptor B7.1

products gene name :

CD80

other gene names :

CD80; CD28LG; CD28LG1; LAB7; B7

uniprot entry name :

CD80_HUMAN

host :

HEk293 Cells

sequence length :

214

purity :

>90%, as determined by SDS-PAGE and HPLC

form :

Recombinant CD80 was lyophilized from 0.2um filtered 50mM Tris, 100mM NaCl, pH 8.0.

storage stability :

Recombinant CD80, as supplied, can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C to -70 degree C for twelve months. Avoid repeated freeze/thaw cycles.

other info1 :

Domain: Ig-like C2. Host Note: Optimized DNA sequence encoding Human extracellular domain of human CD80 including a C-terminal 6His tag was expressed in HEK293 cells. Biological Activity: The activity was tested by immobilized recombinant CD80 (1ug/ml) ability to bind recombinant human CD28 in functional ELISA.

other info2 :

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Molecular Weight Note: Recombinant CD80 is a monomer protein consisting of 214 amino acid residue subunits, due to glycosylation migrates as an approximately 45 kDa protein on SDS-PAGE.

products categories :

Receptors CD

products description :

CD80 is a monomeric surface protein found on activated APCs. The extracellular region of CD80 consists of two highly glycosylated Ig- like extracellular domains, which have defined regions for binding its ligands, CD28 or CTLA-4, on T cells.One ligand of CD80, CD28, provides critical costimulatory signals to activated T cells to increase cytokine production, cell proliferation, and cell viability, while the other ligand, CTLA-4, may mediate a growth-inhibitory signal.The same regions of CD80 are involved in binding to both CD28 and CTLA-4, but CTLA-4 binds to CD80 with about a 20-fold higher avidity than CD28 does. This suggests that low levels of CTLA-4 expressed on the cell surface can compete for CD80 binding even when CD28 is expressed at much higher levels.

products references :

tumor cell programmed death ligand 1-mediated t cell suppression is overcome by coexpression of CD80 . J. Immunol., Jun 2011; 186: 6822 - 6829. toll-like receptor 3 ligands induce CD80 expression in human podocytes via an nf-b-dependent pathway . Nephrol. Dial. Transplant., May 2011; 10.1093/ndt/gfr271. trans-endocytosis of CD80 and cd86: a molecular basis for the cell-extrinsic function of ctla-4 . Science, Apr 2011; 332: 600 - 603. critical role of transcription factor pu.1 in the expression of CD80 and cd86 on dendritic cells . Blood, Feb 2011; 117: 2211 - 2222. enhancement of suboptimal cd8 cytotoxic t cell effector function in vivo using antigen-specific CD80 defective t cells . J. Immunol., Jan 2011; 186: 291 - 304.

ncbi gi num :

4885123

ncbi acc num :

NP_005182.1

ncbi gb acc num :

NM_005191.3

uniprot acc num :

P33681

ncbi mol weight :

33,048 Da

ncbi pathways :

Adaptive Immunity Signaling Pathway (366160); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); CD28 Co-stimulation Pathway (119553); CD28 Dependent PI3K/Akt Signaling Pathway (119554); CD28 Dependent Vav1 Pathway (119555); CTLA4 Inhibitory Signaling Pathway (119556); Cell Adhesion Molecules (CAMs) Pathway (83069)

ncbi summary :

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]

uniprot summary :

CD80: Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. Protein type: Membrane protein, integral; Immunoglobulin superfamily. Chromosomal Location of Human Ortholog: 3q13.3-q21. Cellular Component: cell surface; integral to membrane; plasma membrane; external side of plasma membrane. Molecular Function: protein binding; coreceptor activity. Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; T cell activation; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of signal transduction; positive regulation of transcription, DNA-dependent; positive regulation of interleukin-2 biosynthetic process; positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell signaling; T cell costimulation; innate immune response; positive regulation of alpha-beta T cell proliferation; positive regulation of T-helper 1 cell differentiation

size1 :

0.05 mg

price1 :

220 USD

size2 :

0.2 mg

price2 :

595

size3 :

1 mg

price3 :

2220

more info or order :

MyBioSource product webpage