protein

Human Tissue Plasminogen Activator Recombinant

MBS553134

0.02 mg

145 USD

Recombinant Protein

Human Tissue Plasminogen Activator Recombinant

[Tissue Plasminogen Activator]

[TPA; tissue plasminogen activator]

[tissue-type plasminogen activator isoform 1 preproprotein; Tissue-type plasminogen activator]

[TPA]

[PLAT; t-PA; t-plasminogen activator; tPA]

TPA_HUMAN

Chinese Hamster Ovary Cells (CHO)

562

>98%, as determined by SDS-PAGE and HPLC

Recombinant tPA was lyophilized from a 1mg/ml 0.2 um filtered solution containing 0.2M L-arginine, 0.1M phosphoric acid.

The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

. Source: Optimized DNA sequence encoding Human Tissue Plasminogen Activator mature chain was expressed in Chinese Hamster Ovary cells. Biological Activity: The activity was determined to be 6 x 10^5 IU/mg

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Molecular Weight Note: Native human Tissue plasminogen Activator, generated by the proteolytic removal of the signal peptide and propeptide, has a calculated molecular mass of approximately 55 kDa. Recombinant tPA is a disulfide-linked monomer protein consisting 527 amino acid residue subunits. and migrates due to glycosylation as an approximately 60kDa protein under non-reducing and reducing conditions in SDS-PAGE.

Cytokines

Tissue plasminogen Activator (abbreviated tPA or PLAT) is a protein involved in the breakdown of blood clots. Specifically, it is a serine protease (EC 3.4.21.68) found on endothelial cells, the cells that line the blood vessels. As an enzyme, it catalyzes the conversion of plasminogen to plasmin, the major enzyme responsible for clot breakdown.

TPA inhibits melanoma growth by inactivation of stat3 through pkc-activated tyrosine phosphatase (s) . J. Biol. Chem., Sep 2009; 10.1074/jbc.M109.001073. a nonfibrin macromolecular cofactor for TPA -mediated plasmin generation following cellular injury . Blood, Aug 2009; 114: 1937 - 1946. rapid lysis of posterior synechiae due to uveitis using a combination of intraocular TPA and posterior sub-tenon s triamcinolone acetonide . Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 4997. stimulation of b-cell lymphoproliferations with cpg-oligonucleotide dsp30 plus il-2 is more effective than with TPA to detect clonal abnormalities. . Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 1480. patients with acute stroke treated with intravenous TPA 3 6 hours after stroke onset: correlations between mr angiography findings and perfusion- and diffusion-weighted imaging in the defuse study . Radiology, Nov 2008; 249: 614 - 623.

4505861

NP_000921.1

NM_000930.3

P00750

Blood Clotting Cascade Pathway (198840); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Dissolution Of Fibrin Clot Pathway (106061); Endochondral Ossification Pathway (198812); Hemostasis Pathway (106028); Selenium Pathway (198825); Senescence And Autophagy Pathway (198780); Signaling By PDGF Pathway (106384)

This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

PLAT: Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration. Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. Belongs to the peptidase S1 family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Protease; EC 3.4.21.68; Vesicle. Chromosomal Location of Human Ortholog: 8p12. Cellular Component: extracellular matrix; extracellular space; cell surface; apical part of cell; cytoplasm; extracellular region; synapse; secretory granule. Molecular Function: protein binding; serine-type endopeptidase activity. Biological Process: negative regulation of proteolysis; response to peptide hormone stimulus; synaptic transmission, glutamatergic; fibrinolysis; response to cAMP; smooth muscle cell migration; response to hypoxia; regulation of synaptic plasticity; protein modification process; platelet-derived growth factor receptor signaling pathway; proteolysis; blood coagulation; transmembrane transport; plasminogen activation. Disease: Thrombophilia, Familial, Due To Decreased Release Of Tissue Plasminogen Activator

0.02 mg

145 USD

0.1 mg

220