protein

Human Soluble CD4 Recombinant

MBS553128

0.02 mg

220 USD

Recombinant Protein

Human Soluble CD4 Recombinant

Soluble CD4

Human CD4 Recombinant; cd4; cd-4

T-cell surface glycoprotein CD4 isoform 1; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3

CD4

CD4

CD4_HUMAN

HEK293 Cells

458

MNRGVPFRHL LLVLQLALLP AATQGKKVVL GKKGDTVELT CTASQKKSIQ FHWKNSNQIK ILGNQGSFLT KGPSKLNDRA DSRRSLWDQG NFPLIIKNLK IEDSDTYICE VEDQKEEVQL LVFGLTANSD THLLQGQSLT LTLESPPGSS PSVQCRSPRG KNIQGGKTLS VSQLELQDSG TWTCTVLQNQ KKVEFKIDIV VLAFQKASSI VYKKEGEQVE FSFPLAFTVE KLTGSGELWW QAERASSSKS WITFDLKNKE VSVKRVTQDP KLQMGKKLPL HLTLPQALPQ YAGSGNLTLA LEAKTGKLHQ EVNLVVMRAT QLQKNLTCEV WGPTSPKLML SLKLENKEAK VSKREKAVWV LNPEAGMWQC LLSDSGQVLL ESNIKVLPTW STPVQPMALI VLGGVAGLLL FIGLGIFFCV RCRHRRRQAE RMSQIKRLLS EKKTCQCPHR FQKTCSPI

>90%, as determined by SDS-PAGE and HPLC

Recombinant CD4 was lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.

Stable for at least 2 year of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Domain: IG. Host Note: Optimized DNA sequence encoding Human CD4 extracellular domain was expressed in HEK293 cells. Activity: Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells. Biological Activiy: The activity was tested by immobilized recombinant CD4(1ug/ml) ability to support adhesion of HeLa cells (5x10^4) cells/well). 40-80% will adhere after 60 mins at 37 deg. C. Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1mg/ml. This solution can then be diluted into other buffers.

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Edotoxin level was found to be less than 0.1ng/ug (1EU/ug). Molecular Weight: Human soluble CD4 is generated by the protolytic removal of the extracellular domain and propeptide. The molecule has a calculated molecular mass of approximately 40 kDa. Recombinant CD4 is a disulfide-linked monomer protein consisting of 376 amino acid residue subunits, and migrates due to glycosylation as an approximately 46 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

Receptors CD

Recombinant Human CD4 is a monomer protein consisting of 376 amino acid residue subunits, due to glycosylation migrates as an approximately 46 kDa protein on SDS-PAGE.

influence of novel CD4 binding-defective hiv-1 envelope glycoprotein immunogens on neutralizing antibody and t-cell responses in nonhuman primates . J. Virol., Feb 2010; 84: 1683 - 1695. vaccination with an adenoviral vector that encodes and displays a retroviral antigen induces improved neutralizing antibody and CD4 + t-cell responses and confers enhanced protection . J. Virol., Feb 2010; 84: 1967 - 1976. defects in il-2r signaling contribute to diminished maintenance of foxp3 expression in CD4 +cd25+ regulatory t-cells of type 1 diabetic subjects . Diabetes, Feb 2010; 59: 407 - 415. cellular immune responses to severe acute respiratory syndrome coronavirus (sars-cov) infection in senescent balb/c mice: CD4 + t cells are important in control of sars-cov infection . J. Virol., Feb 2010; 84: 1289 - 1301. decrease of initial CD4 + t-cell counts at the time of diagnosis of hiv infection in korea; 1988 2006 . Int J STD AIDS, Feb 2010; 21: 120 - 125.

10835167

NP_000607.1

NM_000616.4

P01730

51,111 Da

Adaptive Immunity Signaling Pathway (366160); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Arf1 Pathway (137927); Binding And Entry Of HIV Virion Pathway (105986); C-MYB Transcription Factor Network Pathway (138073); CXCR4-mediated Signaling Events Pathway (137910); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Costimulation By The CD28 Family Pathway (119552)

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120. Protein type: Membrane protein, integral; Cell surface. Chromosomal Location of Human Ortholog: 12p13.31. Cellular Component: T cell receptor complex; endoplasmic reticulum membrane; endoplasmic reticulum lumen; early endosome; integral to membrane; plasma membrane; lipid raft; external side of plasma membrane. Molecular Function: protein binding; protein homodimerization activity; transmembrane receptor activity; enzyme binding; zinc ion binding; extracellular matrix structural constituent; coreceptor activity; receptor activity; glycoprotein binding; protein kinase binding; MHC class II protein binding. Biological Process: maintenance of cellular protein localization; viral reproduction; positive regulation of interleukin-2 biosynthetic process; T cell selection; cytokine production; positive regulation of calcium-mediated signaling; induction by virus of cell-cell fusion in host; signal transduction; defense response to Gram-negative bacterium; T cell receptor signaling pathway; enzyme linked receptor protein signaling pathway; regulation of defense response to virus by virus; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; protein palmitoleylation; T cell costimulation; innate immune response; immune response; entry into host cell; transmembrane receptor protein tyrosine kinase signaling pathway; T cell differentiation; regulation of T cell activation. Disease: Okt4 Epitope Deficiency

0.02 mg

220 USD

0.1 mg

595

0.5 mg

2220