Human CD16b Recombinant (FcgR3b) | MyBioSource MBS553103 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Human CD16b Recombinant (FcgR3b)

catalog :

MBS553103

quantity :

0.02 mg

price :

220 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS553103

products type :

Recombinant Protein

products full name :

Human CD16b Recombinant (FcgR3b)

products short name :

CD16b (FcgR3b)

products name syn :

Human CD16b Recombinant (FcgrR3b) ; cd16; cd-16

other names :

low affinity immunoglobulin gamma Fc region receptor III-B isoform 2; Low affinity immunoglobulin gamma Fc region receptor III-B; Fc-gamma RIII-beta; Fc-gamma RIII; Fc-gamma RIIIb; FcRIII; FcRIIIb; FcR-10; IgG Fc receptor III-1

products gene name :

CD16

other gene names :

FCGR3B; CD16B; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIb; FcRIII; FcRIIIb

uniprot entry name :

FCG3B_HUMAN

host :

HEk293 Cells

sequence length :

233

purity :

>95%, as determined by SDS-PAGE and HPLC

form :

Recombinant CD16b is supplied as a lyophilized 0.2 um filtered PBS solution, pH7.4 containing 5% trehalose.

storage stability :

Recombinant CD16b, as supplied, can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree to -70 degree C for twelve months. Avoid repeated freeze/thaw cycles.

other info1 :

Domain: Ig-like C2. Host Note: Optimized DNA sequence encoding Human extracellular domain of human CD16b including a C-terminal 6His tag was expressed in HEK293 cells.

other info2 :

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. Molecular Weight Note: Recombinant CD16b is a monomer protein consisting of 195 amino acid residue subunits, due to glycosylation migrates as an approximately 50 kDa protein on SDS-PAGE.

products categories :

Receptors CD

products description :

Human CD16 is encoded by two genes, Fc RIIIA and Fc RIIIB. Fc RIIIa is a transmembranous isoform of the receptor expressed on the surface of natural killer (NK) cells, macrophages, a subset of monocytes and of T cells in association with dimers of the -chain of Fc RI and/or -chain of T-cell receptors. Fc RIIIb is a glycosylphosphatidylinositol -anchored isoform restricted to neutrophils. Fc RIII plays a significant role in the clearance of immune complexes, antibody- dependent cellular cytotoxicity of K/NK cells,phagocytosis and antigen presentation.

products references :

human basophils express the glycosylphosphatidylinositol-anchored low-affinity igg receptor fcriiib ( CD16b ) . J. Immunol., Feb 2009; 182: 2542 - 2550.

ncbi gi num :

132814489

ncbi acc num :

NP_000561.3

ncbi gb acc num :

NM_000570.4

uniprot acc num :

O75015

ncbi mol weight :

26,216 Da

ncbi pathways :

Leishmaniasis Pathway (144181); Leishmaniasis Pathway (144028); Natural Killer Cell Mediated Cytotoxicity Pathway (83079); Natural Killer Cell Mediated Cytotoxicity Pathway (490); Osteoclast Differentiation Pathway (193147); Osteoclast Differentiation Pathway (193096); Phagosome Pathway (153910); Phagosome Pathway (153859); Staphylococcus Aureus Infection Pathway (172846); Staphylococcus Aureus Infection Pathway (171867)

ncbi summary :

The protein encoded by this gene is a low affinity receptor for the Fc region of gamma immunoglobulins (IgG). The encoded protein acts as a monomer and can bind either monomeric or aggregated IgG. This gene may function to capture immune complexes in the peripheral circulation. Several transcript variants encoding different isoforms have been found for this gene. A highly-similar gene encoding a related protein is also found on chromosome 1. [provided by RefSeq, Aug 2012]

uniprot summary :

Function: Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils. Subunit structure: Monomer. Interacts with INPP5D/SHIP1 . By similarity. Subcellular location: Cell membrane; Lipid-anchor GPI-anchor. Secreted. Note: Secreted after cleavage. Tissue specificity: Expressed specifically by polymorphonuclear leukocytes (neutrophils). Also expressed by stimulated eosinophils. Post-translational modification: Glycosylated. Glycosylation plays an inhibitory role in the interaction with IgG3.The soluble form is produced by a proteolytic cleavage. Polymorphism: There are three allelic forms of FCGR3B: FCGR3B*01 (NA-1), FCGR3B*02 (HNA-1b, NA-2) (shown here) and SH. FCGR3B*01 and FCGR3B*02 are detectable with antibodies against the biallelic neutrophil-specific antigen system NA. The more active FCGR3B*01 allele has been associated with severe renal disease in certain systemic vasculitides. Miscellaneous: Encoded by one of two nearly indentical genes: FCGR3A and FCGR3B (Shown here) which are expressed in a tissue-specific manner. The 'Phe-203' in FCGR3A determines the transmembrane domains whereas the Ser-203 in FCGR3B determines the GPI-anchoring. Sequence similarities: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

size1 :

0.02 mg

price1 :

220 USD

size2 :

0.1 mg

price2 :

540

size3 :

0.5 mg

price3 :

1915

more info or order :

MyBioSource product webpage