Human FLT3 Ligand Recombinant | MyBioSource MBS553096 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Human FLT3 Ligand Recombinant

catalog :

MBS553096

quantity :

0.002 mg

price :

145 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS553096

products type :

Recombinant Protein

products full name :

Human FLT3 Ligand Recombinant

products short name :

FLT3 Ligand

products name syn :

flt3lg; flt3 ligand; flt-3l

other names :

fms-related tyrosine kinase 3 ligand; Fms-related tyrosine kinase 3 ligand; SL cytokine

products gene name :

FLT3

other gene names :

FLT3LG; Flt3 ligand; Flt3L

uniprot entry name :

FLT3L_HUMAN

host :

E Coli

sequence length :

235

sequence :

MTVLAPAWSP TTYLLLLLLL SSGLSGTQDC SFQHSPISSD FAVKIRELSD YLLQDYPVTV ASNLQDEELC GGLWRLVLAQ RWMERLKTVA GSKMQGLLER VNTEIHFVTK CAFQPPPSCL RFVQTNISRL LQETSEQLVA LKPWITRQNF SRCLELQCQP DSSTLPPPWS PRPLEATAPT APQPPLLLLL LLPVGLLLLA AAWCLHWQRT RRRTPRPGEQ VPPVPSPQDL LLVEH

purity :

>97%, as determined by SDS-PAGE and HPLC

form :

Recombinant FLT-3 Ligand was lyophilized from a 0.2 um filtered PBS solution pH 7.0.

storage stability :

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

other info1 :

Domain: four helix cytokine core. Host Note: Optimized DNA sequence encoding Human FLT-3 Ligand mature chain was expressed in Escherichia Coli.

other info2 :

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Molecular Weight Note: Native human FLT3 Ligand is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 17 kDa. Recombinant FLT-3 Ligand is a disulfide-linked monomeric protein consisting of 156 amino acid residue subunits, and migrates as an approximately 17 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.

products categories :

Cytokines

products description :

FLT-3 ligand promotes long- term expansion and differentiation of human pro-B-cells in the presence of IL7 or in combination of IL7 and IL3.The human flt-3 ligand also stimulates the proliferation of cells expressing murine flt-3 receptors.n combination with SCF and IL3 flt-3 ligand can cause expansion of cells with the marker spectrum CD34 (+) CD38 (-). Alone, flt- 3 ligand supports the survival of precursors in the lineage of blood-forming cells such as CFU-GM, CFU- GEMM, and the very primitive high proliferative potential colony-forming cells, HPP- CFC. flt-3 ligand only has marginal effects on progenitors for erythroid cells and megakaryocytes.

products references :

tumor vaccines expressing FLT3 Ligand synergize with ctla-4 blockade to reject preimplanted tumors . Cancer Res., Sep 2009; 10.1158/0008-5472.CAN-08-3289. the concerted action of gm-csf and flt3-ligand on in vivo dendritic cell homeostasis . Blood, Jul 2009; 114: 835 - 843. ectodomain shedding of FLT3 Ligand is mediated by tnf- converting enzyme . J. Immunol., Jun 2009; 182: 7408 - 7414. transplanted cd34+ cells stimulated with FLT3 Ligand and granulocyte-colony stimulating factor display an impaired early reconstitution capacity . Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 4591. hematopoietic stem - and progenitor cells are differentially mobilized in response to flt3-ligand . Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 4782.

ncbi gi num :

325197197

ncbi acc num :

NP_001191431.1

ncbi gb acc num :

NM_001204502.1

uniprot acc num :

P49771

ncbi mol weight :

26,416 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Pathways In Cancer (83105)

ncbi summary :

Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE; MIM 604682)-positive tissue counterparts (summary by Sathaliyawala et al., 2010 [PubMed 20933441]).[supplied by OMIM, Jan 2011]

uniprot summary :

FLT3LG: Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Ligand, receptor tyrosine kinase; Cytokine; Membrane protein, integral. Chromosomal Location of Human Ortholog: 19q13.3. Cellular Component: extracellular space; membrane; integral to membrane. Molecular Function: protein homodimerization activity; cytokine activity; receptor tyrosine kinase binding; receptor binding. Biological Process: regulation of myeloid dendritic cell activation; positive regulation of natural killer cell differentiation; lymphocyte differentiation; embryonic hemopoiesis; positive regulation of cell proliferation; positive regulation of protein amino acid phosphorylation; signal transduction; positive regulation of myoblast differentiation

size1 :

0.002 mg

price1 :

145 USD

size2 :

0.01 mg

price2 :

220

more info or order :

MyBioSource product webpage