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Rat basic Fibroblast Growth Factor Recombinant

MBS553082

0.01 mg

145 USD

MBS553082

Recombinant Protein

Rat basic Fibroblast Growth Factor Recombinant

basic Fibroblast Growth Factor

Rat bFGF recombinant; fgf-2; fibrobalst growth factor; fgf2; bfgf

fibroblast growth factor 2; Fibroblast growth factor 2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2

bFGF

Fgf2; Fgf-2; FGF-2; bFGF; HBGF-2

FGF2_RAT

E Coli

154

MAAGSITSLP ALPEDGGGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHVK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTEEC FFFERLESNN YNTYRSRKYS SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

>96%, as determined by SDS-PAGE and HPLC

Rat Basic FGF was lyophilized from a 0.2 um filtered PBS solution pH 7.5.

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Domain: HBGF/IL1. Host Note: Optimized DNA sequence encoding Rat basic Fibroblast Growth Factor mature chain was expressed in Escherichia Coli.

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Molecular Weight Note: Native Rat FGF basic is generated by the proteolytic removal of the signal peptide. and propeptide,the molecule has a calulated mass of 16 kDa. Recombinant Rat bFGF is a disulfide-linked homodimeric protein consisting of 146 amino acid residue subunits, and migrates as an approximately 16 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

Cytokines

basic Fibroblast Growth Factor is found in almost all tissues of mesodermal and neuroectodermal origin and also in tumors derived from these tissues. Endothelial cells produce large amounts of this factor. Some bFGF is associated with the extracellular matrix of the subendothelial cells. Many cells express bFGF only transiently and store it in a biologically inactive form. The mechanism by which the factor is released by the cells is not known. It is released after tissue injuries and during inflammatory processes. FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation. bFGF stimulates the growth of fibroblasts, myoblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, chondrocytes, and many other cell types.

expression of bFGF and il-1 alpha in tears of galactosemic rats: implications for wound healing and neovascularization . Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 545. p53 is necessary for bFGF -induced lens differentiation . Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 4369. regulation of basic fibroblast growing factor ( bFGF ) by retinal glial (m ller) cells under severe retinal ischemia . Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 2926. natural history of progressive experimental cnv in the rabbit after sustained release of both vegf and bFGF within the supra-choroidal space . Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 2970. keratocyte-associated mt1-mmp modulates bFGF -induced vegf expression . Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 4967.

9506599

NP_062178.1

NM_019305.2

P13109

17,139 Da

Downstream Signaling Of Activated FGFR Pathway 368292!!Endochondral Ossification Pathway 219771!!FGFR Ligand Binding And Activation Pathway 368281!!FGFR1 Ligand Binding And Activation Pathway 368282!!FGFR1b Ligand Binding And Activation Pathway 368283!!FGFR1c Ligand Binding And Activation Pathway 368284!!FGFR2 Ligand Binding And Activation Pathway 368285!!FGFR2c Ligand Binding And Activation Pathway 368287!!FGFR3 Ligand Binding And Activation Pathway 368288!!FGFR3c Ligand Binding And Activation Pathway 368290

Function: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro . By similarity. Subunit structure: Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2 . By similarity. Subcellular location: Secreted . By similarity. Nucleus . By similarity. Note: Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane . By similarity. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57 . By similarity. Tissue specificity: Found in all tissues examined. Post-translational modification: Phosphorylation at Tyr-81 regulates FGF2 unconventional secretion . By similarity. Sequence similarities: Belongs to the heparin-binding growth factors family.

0.01 mg

145 USD