protein

Human CD124 Recombinant (IL4Ra)

MBS553069

0.01 mg

220 USD

Recombinant Protein

Human CD124 Recombinant (IL4Ra)

CD124 (IL4Ra)

Human CD124 Recombinant (IL4Ra) ; CD124; IL4Ra; IL4R; il-4r

interleukin-4 receptor subunit alpha isoform a; Interleukin-4 receptor subunit alpha; IL-4-binding protein

CD124

IL4R; IL4RA; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; Soluble IL-4 receptor subunit alpha; Soluble IL-4R-alpha; sIL4Ralpha/prot; IL4-BP

IL4RA_HUMAN

HEk293 Cells

825

>98%, as determined by SDS-PAGE and HPLC

Recombinant CD124 is supplied as a 0.2 um filtered PBS solution, pH7.2.

Recombinant CD124, as supplied, can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C to -70 degree C for twelve months. Avoid repeated freeze/thaw cycles.

Domain: Ig-like C2. Host Note: Optimized DNA sequence encoding Human extracellular domain of human CD124 including a C-terminal 6His tag was expressed in HEK293 cells.

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Molecular Weight Note: Recombinant CD124 is a monomer protein consisting of 218 amino acid residue subunits, due to glycosylation migrates as an approximately 43-48 kDa protein on SDS-PAGE.

Receptors CD

4557669

NP_000409.1

NM_000418.3

P24394

89,658 Da

Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Hematopoietic Cell Lineage Pathway 83078!!Hematopoietic Cell Lineage Pathway 489!!IL-4 Signaling Pathway 198760!!IL4-mediated Signaling Events Pathway 137933!!Inflammatory Response Pathway 198766!!Jak-STAT Signaling Pathway 83077!!Jak-STAT Signaling Pathway 488

Function: Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. Ref.9Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells. Ref.9. Subunit structure: The functional IL4 receptor is formed by initial binding of IL4 to IL4R. Subsequent recruitment to the complex of the common gamma chain, in immune cells, creates a type I receptor and, in non-immune cells, of IL13RA1 forms a type II receptor. IL4R can also interact with the IL13/IL13RA1 complex to form a similar type II receptor. Interacts with PIK3C3 . By similarity. Interacts with the SH2-containing phosphatases, PTPN6/SHIP1, PTPN11/SHIP2 and INPP5D/SHIP . By similarity. Interacts with JAK1 through a Box 1-containing region; inhibited by SOCS5. Interacts with SOCS5; inhibits IL4 signaling . By similarity. Ref.11 Ref.12 Ref.13 Ref.16. Subcellular location: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Tissue specificity: Isoform 1 and isoform 2 are highly expressed in activated T-cells. Domain: The extracellular domain represents the IL4 binding protein (IL4BP). Ref.10 Ref.14The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. Ref.10 Ref.14The box 1 motif is required for JAK interaction and/or activation. Ref.10 Ref.14Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. Ref.10 Ref.14. Post-translational modification: On IL4 binding, phosphorylated on C-terminal tyrosine residues. Phosphorylation on any one of tyrosine residues, Tyr-575, Tyr-603 or Tyr-631, is required for STAT6-induced gene induction. Ref.13The soluble form (sIL4R/IL4BP) can also be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase. Polymorphism: Allelic variants in IL4RA are associated with a susceptibility to atopy, an immunological condition that can lead to clinical symptoms such as allergic rhinitis, sinusitis, asthma and eczema.Allelic variants in IL4RA are associated with cedar pollen sensitization. Individuals develop Japanese cedar pollinosis with increased exposure to cedar pollen. Japanese cedar pollinosis is a type I allergic disease with ocular and nasal symptoms that develop paroxysmally on contact with Japanese cedar pollen. These symptoms, which occur seasonally each year, are typical features of allergic rhinitis, such as sneezing, excessive nasal secretion, nasal congestion, and conjunctival itching. Sequence similarities: Belongs to the type I cytokine receptor family. Type 4 subfamily.Contains 1 fibronectin type-III domain.

0.01 mg

220 USD