Cytomegalovirus pp65 (UL83) Recombinant | MyBioSource MBS553061 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Cytomegalovirus pp65 (UL83) Recombinant

catalog :

MBS553061

quantity :

0.5 mg

price :

775 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS553061

products type :

Recombinant Protein

products full name :

Cytomegalovirus pp65 (UL83) Recombinant

products short name :

Cytomegalovirus pp65 (UL83)

products name syn :

CMV; pp65; ul83

other names :

65 kDa phosphoprotein; 65 kDa phosphoprotein; 65 kDa matrix phosphoprotein; Phosphoprotein UL83; Tegument protein UL83

products gene name :

CMV pp65

other gene names :

UL83; pp65

uniprot entry name :

PP65_HCMVA

host :

E Coli

sequence length :

561

purity :

>95%, as determined by SDS-PAGE.

form :

Lyophilized from 25mM Tris-Hcl pH 7.2, 1mM EDTA and 50% glycerol.

storage stability :

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this protein can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

app notes :

CMV pp65 antigen is suitable for ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems.

other info1 :

Host Note: DNA sequence encoding immunodominant fragment of Cytomegalovirus 65kDa tegument phosphoprotein was expressed in Escherichia Coli.

other info2 :

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Molecular Weight Note: Recombinant CMV pp65 (UL83) encodes the immunodominant sequences of the Cytomegalovirus matrix phosphoprotein. This molecule has a calculated mass of approximately 22kDa. Recombinant CMV pp65 contains an N terminal GST purification tag and migrates as an approximately 52 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.

products categories :

Viral Antigens

products description :

Human cytomegalovirus (HCMV) lower matrix protein pp65 (ppUL83), a major target for host anti-HCMV response, is the product of a late-expressed virus mRNA (reviewed in Spaete et al., 1994). pp65 replenishes a type of HCMV non-infectious particle, the dense bodies, which are secreted by infected cells along with virionsThe phosphoprotein pp65 (ppUL83) of human cytomegalovirus (HCMV) is abundantly synthesized during lytic infection in cultured fibroblasts. As a major constituent of extracellular particles, it gains entry to infected cells immediately after adsorption and subsequently translocates to the cell nucleus.

products references :

evaluation of different co-stimulatory signals in the priming and expansion of hla-b*0702/cmv_pp65 restricted ctls after stimulation with aapc . Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 4902. donor lymphocytes stimulated with CMV pp65 gene modified dendritic cells for prophylaxis of cmv following allogeneic haemopoietic stem cell transplantation. . Blood (ASH Annual Meeting Abstracts), Nov 2007; 110: 2989. rapid generation of cytomegalovirus specific t lymphocytes using commercially available CMV pp65 peptides. . Blood (ASH Annual Meeting Abstracts), Nov 2006; 108: 5144. generation of gmp-grade CMV pp65 -specific cd8+ and cd4+ donor t cell lines for treatment of cmv reactivation after transplantation. . Blood (ASH Annual Meeting Abstracts), Nov 2006; 108: 2931. functional comparison and longitudinal assessment of tri-functional t-cells recognizing CMV pp65 and ie-1 polypeptides in hematopoietic stem cell and solid organ transplant recipients. . Blood (ASH Annual Meeting Abstracts), Nov 2006; 108: 2936.

ncbi gi num :

130714

ncbi acc num :

P06725.2

uniprot acc num :

P06725

ncbi mol weight :

62,898 Da

ncbi pathways :

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

uniprot summary :

Function: Counteracts the host antiviral immune response when activated and phosphorylated, by preventing IRF3 from entering the nucleus. Also participates in the transactivation of viral major immediate-early genes by the recruitment of host IFI16 to the promoters pf these genes. Ref.9 Ref.11. Subunit structure: Interacts with host NCL/nucleolin. Interacts with host IFI16. Ref.11 Ref.12. Subcellular location: Virion tegument . Potential. Host nucleus. Host cytoplasm. Note: As part of the incoming virion, pp65 is targeted to the nucleus immediately after infection. The newly synthesized pp65 is observed in the nucleus until some time after 48 hours postinfection. Thereafter, pp65 is probably exported and accumulates in the cytoplasm. Also found in dense bodies. Ref.6 Ref.10. Post-translational modification: Phosphorylation may play a role in the localization of the protein. Sequence similarities: Belongs to the herpesviridae pp65 family.

size1 :

0.5 mg

price1 :

775 USD

size2 :

1 mg

price2 :

1235

more info or order :

MyBioSource product webpage