protein

Human Insulin Like Growth Factor-I Recombinant

MBS553053

0.02 mg

145 USD

Recombinant Protein

Human Insulin Like Growth Factor-I Recombinant

Insulin Like Growth Factor-I

igf-1; insulin like; igf1; igf-i

insulin-like growth factor 1 isoform 4 preproprotein; Insulin-like growth factor I; Mechano growth factor; MGF; Somatomedin-C

IGF-I

IGF1; IBP1; IGF-I; MGF

IGF1_HUMAN

E Coli

153

MGKISSLPTQ LFKCCFCDFL KVKMHTMSSS HLFYLALCLL TFTSSATAGP ETLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSARS VRAQRHTDMP KTQKEVHLKN ASRGSAGNKN YRM

>95%, as determined by SDS-PAGE and HPLC

Recombinant Human IGF1 was lyophilized from a 0.2 um filtered PBS solution, pH 7.4.

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Domain: Insulin. Host Note: Optimized DNA sequence encoding Human Insulin Growth Factor -1mature chain was expressed in Escherichia Coli.

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Molecular Weight Note: Native Human Insulin Growth Factor-1 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 8 kDa. Recombinant Human IGF-1 is a disulfide-linked monomeric protein consisting of 71 amino acid residue subunits. IGF-1 migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

Cytokines

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I is synthesized as two precursor isoforms with N- and alternate C- terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C- terminal regions. Mature human IGF-I shares 94% and 96% aa sequence identity with mouse and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF-II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects.

statement by the growth hormone research society on the gh/ IGF-I axis in extending health span . J Gerontol A Biol Sci Med Sci, Oct 2009; 64A: 1039 - 1044. partial primary deficiency of insulin-like growth factor (igf) -i activity associated with igf1 mutation demonstrates its critical role in growth and brain development . J. Clin. Endocrinol. Metab., Sep 2009; 10.1210/jc.2009-0452. myostatin inhibits IGF-I induced myotube hypertrophy through akt . Am J Physiol Cell Physiol, Sep 2009; 10.1152/ajpcell.00043.2009. the insulin-like growth factor (igf) -i e-peptides modulate cell entry of the mature IGF-I protein . Mol. Biol. Cell, Sep 2009; 20: 3810 - 3817. elevated levels of insulin-like growth factor (igf) -i in serum rescue the severe growth retardation of IGF-I null mice . Endocrinology, Sep 2009; 150: 4395 - 4403.

11024682

NP_000609.1

NM_000618.3

P01343

21,841 Da

Adipogenesis Pathway (198832); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Androgen Receptor Signaling Pathway (198806); Apoptosis Pathway (198797); Diabetes Pathways (105902); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Endochondral Ossification Pathway (198812); Focal Adhesion Pathway (198795)

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]

IGF1: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency). IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. Belongs to the insulin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 12q23.2. Cellular Component: insulin-like growth factor binding protein complex; extracellular space; plasma membrane; extracellular region. Molecular Function: integrin binding; insulin-like growth factor receptor binding; protein binding; growth factor activity; hormone activity; insulin receptor binding. Biological Process: muscle development; positive regulation of transcription, DNA-dependent; chondroitin sulfate proteoglycan biosynthetic process; glycolate metabolic process; exocrine pancreas development; water homeostasis; positive regulation of glucose import; positive regulation of fibroblast proliferation; proteoglycan biosynthetic process; inner ear development; positive regulation of DNA binding; muscle hypertrophy; platelet activation; positive regulation of mitosis; positive regulation of protein import into nucleus, translocation; regulation of establishment and/or maintenance of cell polarity; positive regulation of phosphoinositide 3-kinase cascade; cell activation; positive regulation of peptidyl-tyrosine phosphorylation; insulin-like growth factor receptor signaling pathway; branching morphogenesis of a tube; response to heat; regulation of gene expression; positive regulation of transcription from RNA polymerase II promoter; alveolus development; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; positive regulation of insulin-like growth factor receptor signaling pathway; myoblast proliferation; positive regulation of smooth muscle cell proliferation; positive regulation of glycogen biosynthetic process; signal transduction; positive regulation of activated T cell proliferation; positive regulation of smooth muscle cell migration; negative regulation of cell proliferation; glial cell differentiation; platelet degranulation; positive regulation of MAPKKK cascade; mammary gland development; positive regulation of cell proliferation; DNA replication; skeletal development; positive regulation of granule cell precursor proliferation; phosphoinositide-mediated signaling; multicellular organism growth; regulation of multicellular organism growth; myotube cell development; satellite cell compartment self-renewal involved in skeletal muscle regeneration; myoblast differentiation; positive regulation of osteoblast differentiation; cell proliferation; positive regulation of protein kinase B signaling cascade; cellular protein metabolic process; positive regulation of tyrosine phosphorylation of Stat5 protein; Ras protein signal transduction; positive regulation of glycolysis; blood vessel remodeling; positive regulation of Ras protein signal transduction; cell motility; blood coagulation; positive regulation of DNA replication. Disease: Insulin-like Growth Factor I Deficiency

0.02 mg

145 USD

0.1 mg

220