protein

Cytomegalovirus glycoprotein B (UL55) Recombinant

MBS553041

0.5 mg

775 USD

Recombinant Protein

Cytomegalovirus glycoprotein B (UL55) Recombinant

Cytomegalovirus glycoprotein B (UL55)

cmv; gb; glycoprotein B

Envelope glycoprotein B; Envelope glycoprotein B

CMV gB

gB; gB

GB_HCMVA

E Coli

906

Immunoreactive with sera of infected HIV individuals

>90%, as determined by SDS-PAGE.

Lyophilized from 25mM Tris-Hcl pH 7.2, 1mM EDTA and 50% glycerol.

12.5 mg/ml

Stable for at least 1 years from date of receipt at -20 degree C.

CMV gB antigen is suitable for ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems.

Host Note: DNA sequence encoding immunodominant fragment of Cytomegalovirus Glycoprotein B was expressed in Escherichia Coli. Presentation: 0.2um filtered 50mM tris HCL pH 8.0, Gluthathione 10mM, Glycerol 50%, NaCl 60mM, sakosyl 0.1%. Endotoxin: Less than 0.1EU/ug as deteremine by LAL method.

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Molecular Weight Note: Recombinant CMV gB (UL55) encodes the immunodominant sequences of the Cytomegalovirus Glycoprotein B. This molecule has a calculated mass of approximately 7kDa. Recombinant CMV Glycoprotein B contains an N terminal GST purification tag and migrates as an approximately 33 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.

Viral Antigens

Recombinant CMV gB encodes the immunodominant sequences of the CMV Glycoprotein B. Recombinant CMV Glycoprotein B contains and N terminal GST purification tag and migrates as an approximately 39kDa protein reducing conditions in SDS-PAGE.

138192

P06473.1

P06473

102,004 Da

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

Function: Envelope glycoprotein that plays a role in host cell entry, cell to-cell virus transmission, and fusion of infected cells. May be involved in the initial attachment via binding to heparan sulfate together with the gM/gN complex that binds heparin with higher affinity. Interacts with host integrin ITGB1, PDGFRA and EGFR that likely serve as postattachment entry receptors. Participates also in the fusion of viral and cellular membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. Ref.6 Ref.12Viral ligand for CD209/DC-SIGN. This interaction allows capture of viral particles by dendritic (DCs) cells and subsequent virus transmission to permissive cells. DCs are professional antigen presenting cells, critical for host immunity by inducing specific immune responses against a broad variety of pathogens. They act as sentinels in various tissues where they entrap pathogens and convey them to local lymphoid tissue or lymph node for establishment of immunity. CMV subverts the migration properties of dendritic cells to gain access to target organs or susceptible cells . By similarity. Ref.6 Ref.12. Subunit structure: Homotrimer; disulfide-linked . By similarity. Interacts with host TLR1 and TLR2. Interacts with host C-type lectin CD209/DC-SIGN. Interacts with host ITGB1, EGFR, and PDGFRA . By similarity. Ref.5 Ref.6 Ref.11 Ref.13. Subcellular location: Virion membrane; Single-pass type I membrane protein . By similarity. Post-translational modification: A proteolytic cleavage by host furin generates two subunits that remain linked by disulfide bonds . By similarity. Sequence similarities: Belongs to the herpesviridae glycoprotein B family.

0.5 mg

775 USD

1 mg

1235