protein

Human Bone Morphogenetic protein-7 Recombinant

MBS553020

0.002 mg

145 USD

Recombinant Protein

Human Bone Morphogenetic protein-7 Recombinant

Bone Morphogenetic protein-7

bmp7; bmp-7; Bone Morphogenetic protein-7; op1

bone morphogenetic protein 7; Bone morphogenetic protein 7; Osteogenic protein 1

BMP-7

BMP7; OP1; BMP-7; OP-1

BMP7_HUMAN

HEK Cells

431

MHVRSLRAAA PHSFVALWAP LFLLRSALAD FSLDNEVHSS FIHRRLRSQE RREMQREILS ILGLPHRPRP HLQGKHNSAP MFMLDLYNAM AVEEGGGPGG QGFSYPYKAV FSTQGPPLAS LQDSHFLTDA DMVMSFVNLV EHDKEFFHPR YHHREFRFDL SKIPEGEAVT AAEFRIYKDY IRERFDNETF RISVYQVLQE HLGRESDLFL LDSRTLWASE EGWLVFDITA TSNHWVVNPR HNLGLQLSVE TLDGQSINPK LAGLIGRHGP QNKQPFMVAF FKATEVHFRS IRSTGSKQRS QNRSKTPKNQ EALRMANVAE NSSSDQRQAC KKHELYVSFR DLGWQDWIIA PEGYAAYYCE GECAFPLNSY MNATNHAIVQ TLVHFINPET VPKPCCAPTQ LNAISVLYFD DSSNVILKKY RNMVVRACGC H

>95%, as determined by SDS-PAGE and HPLC

Recombinant BMP-7 was lyophilized from a 0.2 um filtered PBS solution pH 7.4.

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Domain: TGFb. Host Note: Optimized DNA sequence encoding Human Bone Morphogenetic protein-7 mature chain was expressed in E. coli.

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Molecular Weight Note: Native human BMP-7 is generated by the proteolytic removal of the signal peptide. and propeptide, the molecule has a calculated molecular mass of approximately 17 kDa. Recombinant BMP-7 is a disulfide-linked homodimeric protein consisting of two 139 amino acid residue subunits, and migrates as an approximately 30 kDa protein under under reducing conditions in SDS-PAGE.

Cytokines

BMP-7 (osteogenic protein- 1) is produced in the kidney and in the nervous system. It is produced also by human fetal trophoblasts in vivo and may be involved in the regulation of the synthesis of chorionic gonadotropin and progesterone by trophoblasts. BMP7 induces new bone growth, and forms heterodimers with BMP2A. The factor is a member of the TGF family of proteins.

differential regulation of e-cadherin and -smooth muscle actin by bmp 7 in human renal proximal tubule epithelial cells and its implication in renal fibrosis. . Am J Physiol Renal Physiol, Sep 2009; 10.1152/ajprenal.90539.2008. heparin cross-linked collagen coated tcp/ha granules as a carrier for BMP-7 . J Bone Joint Surg Br Proceedings, Sep 2009; 91-B: 445. BMP-7 fails to attenuate tgf- 1-induced epithelial-to-mesenchymal transition in human proximal tubule epithelial cells . Nephrol. Dial. Transplant., May 2009; 24: 1406 - 1416. the treatment of recalcitrant long bone non-unions with bone morphogenetic protein-7 ( BMP-7 ) . J Bone Joint Surg Br Proceedings, May 2009; 91-B: 268. BMP-7 induces wound healing without neovascularization and opacification in a corneal alkali injured mouse model . Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 4549.

4502427

NP_001710.1

NM_001719.2

P18075

49,313 Da

ALK2 Signaling Events Pathway (137983); BMP Receptor Signaling Pathway (137948); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Endochondral Ossification Pathway (198812); Hedgehog Signaling Pathway (83063); Hedgehog Signaling Pathway (474); TGF-beta Signaling Pathway (83064); TGF-beta Signaling Pathway (475)

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development and possible bone inductive activity. [provided by RefSeq, Jul 2008]

BMP7: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with TWSG1. Expressed in the kidney and bladder. Lower levels seen in the brain. Belongs to the TGF-beta family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 20q13. Cellular Component: extracellular matrix; extracellular space; extracellular region. Molecular Function: heparin binding; protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding. Biological Process: axon guidance; response to peptide hormone stimulus; negative regulation of MAP kinase activity; extracellular matrix organization and biogenesis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of NF-kappaB import into nucleus; embryonic pattern specification; response to estradiol stimulus; negative regulation of cell cycle; regulation of apoptosis; BMP signaling pathway; response to vitamin D; epithelial cell differentiation; ureteric bud development; dendrite development; mesonephros development; skeletal development; embryonic limb morphogenesis; ossification; positive regulation of heterotypic cell-cell adhesion; positive regulation of bone mineralization; odontogenesis of dentine-containing teeth; positive regulation of osteoblast differentiation; negative regulation of neuron differentiation; mesenchymal cell differentiation; branching morphogenesis of a tube; mesoderm formation; inhibition of NF-kappaB transcription factor; cartilage development; negative regulation of mitosis; epithelial to mesenchymal transition; negative regulation of phosphorylation; negative regulation of Notch signaling pathway; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; embryonic camera-type eye morphogenesis; negative regulation of transcription, DNA-dependent; metanephros development; neurite morphogenesis; growth

0.002 mg

145 USD

0.01 mg

220