protein

Human ITAC (CXCL11) Recombinant

MBS553011

0.005 mg

145 USD

Recombinant Protein

Human ITAC (CXCL11) Recombinant

ITAC (CXCL11)

itac; i-tac; cxcl-11; cxcl11; SCYB11; SCYB9B

C-X-C motif chemokine 11; C-X-C motif chemokine 11; Beta-R1; H174; Interferon gamma-inducible protein 9; IP-9; Interferon-inducible T-cell alpha chemoattractant; I-TAC; Small-inducible cytokine B11

ITAC

CXCL11; ITAC; SCYB11; SCYB9B; IP-9; I-TAC

CXL11_HUMAN

E Coli

94

EVIITLKENK GQRCLNPKSK QARLIIKKVE RKNF

>98%, as determined by SDS-PAGE and HPLC

Recombinant ITAC was lyophilized from a 0.2 um filtered 20mM PB,100mM NaCl solution pH 7.5.

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Host Note: Optimized DNA sequence encoding Human ITAC (CXCL11) mature chain was expressed in Escherichia Coli.

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Molecular Weight Note: Human ITAC, generated by the proteolytic removal of the signal peptide. and propeptide and has a calculated molecular mass of approximately 8 kDa. Recombinant CXCL11 is a disulfide-linked monomeric protein consisting of 74 amino acid residue subunits and migrates as an approximately 8 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.

Cytokines

The CXC chemokine ligand 11 (CXCL11) or IFN-inducible T- cell -chemoattractant (I- TAC) belongs to the CXC chemokine family characterized by the presence of 1 amino acid in between the 2 NH2-terminal cysteines. CXCL11 is produced by a variety of cells including leukocytes, fibroblasts, and endothelial cells upon stimulation with interferons (IFNs). Simultaneous stimulation of fibroblasts or endothelial cells with IFN-gamma and interleukin-1b or the TLR3 ligand double-stranded RNA resulted in a synergistic increase of CXCL11 production. CXCL11 attracts activated T- helper 1 (Th1) lymphocytes and natural killer (NK) cells. Like CXCL9 or monokine induced by IFN- gamma (Mig) and CXCL10 or IFN-gamma-inducible protein- 10 (IP-10), CXCL11 signals through CXC chemokine receptor 3 (CXCR3).

monokine induced by interferon (ifn) (cxcl9) and ifn inducible t-cell -chemoattractant ( CXCL11 ) involvement in graves disease and ophthalmopathy: modulation by peroxisome proliferator-activated receptor- agonists . J. Clin. Endocrinol. Metab., May 2009; 94: 1803 - 1809. th1 cells express ifn-gamma, and m1 macrophages ccl19/ CXCL11 and il-17/il-18/il-23 in sympathetic ophthalmia . Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 6014. citrullination of cxcl10 and CXCL11 by peptidylarginine deiminase: a naturally occurring posttranslational modification of chemokines and new dimension of immunoregulation . Blood, Oct 2008; 112: 2648 - 2656. elr-negative cxc chemokine CXCL11 (ip-9/i-tac) facilitates dermal and epidermal maturation during wound repair . Am. J. Pathol., Sep 2008; 173: 643 - 652. matrix metalloproteinase processing of CXCL11 /i-tac results in loss of chemoattractant activity and altered glycosaminoglycan binding . J. Biol. Chem., Jul 2008; 283: 19389 - 19399.

4885589

NP_005400.1

NM_005409.4

O14625

10,365 Da

CXCR3-mediated Signaling Events Pathway (138011); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020)

Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC. This antimicrobial gene is a CXC member of the chemokine superfamily. Its encoded protein induces a chemotactic response in activated T-cells and is the dominant ligand for CXC receptor-3. The gene encoding this protein contains 4 exons and at least three polyadenylation signals which might reflect cell-specific regulation of expression. IFN-gamma is a potent inducer of transcription of this gene. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

CXCL11: Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses. Belongs to the intercrine alpha (chemokine CxC) family. Protein type: Secreted; Motility/polarity/chemotaxis; Chemokine; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q21.2. Cellular Component: extracellular space; extracellular region. Molecular Function: heparin binding; protein binding; CXCR3 chemokine receptor binding; chemokine activity. Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling; immune response; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of cAMP metabolic process; positive regulation of release of sequestered calcium ion into cytosol; inflammatory response; signal transduction; chemotaxis; regulation of cell proliferation

0.005 mg

145 USD

0.02 mg

220