Rabbit anti Tyrosine Hydroxylase Polyclonal | MyBioSource MBS551076 product information

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product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti Tyrosine Hydroxylase Polyclonal

catalog :

MBS551076

quantity :

0.1 mg

price :

280 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

product information

catalog number :

MBS551076

products type :

Antibody

products full name :

Rabbit anti Tyrosine Hydroxylase Polyclonal

products short name :

Tyrosine Hydroxylase

products name syn :

Rabbit anti Tyrosine Hydroxylase Polyclonal purified; Tyrosine Hydroxylase; TYh; polyclonal

other names :

tyrosine 3-monooxygenase isoform b; Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase

products gene name :

TYH

other gene names :

TH; TYH; TH

uniprot entry name :

TY3H_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

497

purity :

Serum IgG fraction was purified by Protein G affinity chromatography.

form :

Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.

storage stability :

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. Shelf Life: >12 months

tested application :

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

app notes :

ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of FFPE Rat Hippocampus sections showing expression of Tyrosine Hydroxylase. It was used as primary antibody, dilution 1:500, DAB staining. Immunohistochemical analysis of FFPE mouse brain sections showing Substantia nigra neurons expressing Tyrosine Hydroxylase. It was used as primary antibody, dilution 1:500, DAB staining.

other info1 :

Source Note: Polyclonal anti Tyrosine Hydroxylase antibody was produced from sera of rabbits immunized with synthetic protein containing Tyrosine Hydroxylase antigenic determinants.

other info2 :

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers

products categories :

Polyclonal

products references :

short-term hypoxia increases Tyrosine Hydroxylase immunoreactivity in rat carotid body . J. Histochem. Cytochem., Jun 2010; 10.1369/jhc.2010.956250. serine 129 phosphorylation reduces the ability of -synuclein to regulate Tyrosine Hydroxylase and protein phosphatase 2a in vitro and in vivo . J. Biol. Chem., Jun 2010; 285: 17648 - 17661. Tyrosine Hydroxylase is expressed during early heart development and is required for cardiac chamber formation . Cardiovasc Res, Jun 2010; 10.1093/cvr/cvq179. Tyrosine Hydroxylase deficiency: a treatable disorder of brain catecholamine biosynthesis . Brain, Jun 2010; 133: 1810 - 1822. electrophysiological and morphological characteristics and synaptic connectivity of Tyrosine Hydroxylase -expressing neurons in adult mouse striatum . J. Neurosci., May 2010; 30: 6999 - 7016.

ncbi gi num :

88900503

ncbi acc num :

NP_000351.2

ncbi gb acc num :

NM_000360.3

uniprot acc num :

P07101

ncbi mol weight :

58,600 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway 138006!!Alpha-synuclein Signaling Pathway 137913!!Amine-derived Hormones Pathway 160983!!Biogenic Amine Synthesis Pathway 198793!!Biosynthesis Of Amine And Peptide Hormones Pathway 366244!!Catecholamine Biosynthesis Pathway 160984!!Dopamine Neurotransmitter Release Cycle Pathway 106523!!Metabolic Pathways 132956!!Metabolism Of Amino Acids And Derivatives Pathway 106169!!Metabolism Of Hormones Pathway 366243

uniprot summary :

Function: Plays an important role in the physiology of adrenergic neurons. Catalytic activity: L-tyrosine + tetrahydrobiopterin + O2 = L-dopa + 4a-hydroxytetrahydrobiopterin. Cofactor: Fe2+ ion. Enzyme regulation: Phosphorylation leads to an increase in the catalytic activity. Pathway: Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. Tissue specificity: Mainly expressed in the brain and adrenal glands. Involvement in disease: Segawa syndrome autosomal recessive (ARSEGS) [MIM:605407]: A form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.11 Ref.12 Ref.14 Ref.15 Ref.16 Ref.18 Ref.21May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. Ref.11. Sequence similarities: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

size :

0.1 mg

price :

280 USD