antibody

Rabbit anti phospho CREB1 Polyclonal

MBS551068

0.1 mg

280 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Rabbit anti phospho CREB1 Polyclonal

phospho CREB1

Rabbit anti phospo CREB1 Polyclonal purified; ser133; creb1; creb-1; polyclonal

cyclic AMP-responsive element-binding protein 1 isoform A; Cyclic AMP-responsive element-binding protein 1

CREB1

CREB-1

CREB1; CREB-1

CREB1_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

327

Serum IgG fraction was purified by Protein G affinity chromatography.

Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. Shelf Life: >12 months

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. WB analysis mouse hippocampal tissue lysate expressing phospho CREB1. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of frozen mouse hyppocampal sections expressing CREB1. Immunohistochemical analysis of FFPE mouse thymocytes expressing phospho CREB-1.

Source Note: Polyclonal p-CREB-1 antibody was produced from sera of mice immunized with synthetic protein containing residues surrounding ser133.

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers

Polyclonal

The ATF/CREB family consists of transcription factors that function through binding to the cAMP responsive element (CRE) palindromic octanucleotide, TGACCTCA. The best characterized members of this gene family include CREB-1, CREB-2, ATF-1,ATF- 2,ATF-3and ATF-4. these transcription factors share highly-related COOH terminal leucine zipper demerization and basic DNA bindings but are highly divergent in their amino terminal domains. Although each of the ATF/CREB proteins bind CREs in their homodimeric form, in cerain instances they also bind as heterodimers, both within the ATF/CREB family and with members of the AP-1 transcription factor family. It has recentlybeen shown that protein kinase A- mediated CREB phosphorylation results in its binding to a 265kDa nuclear protein designated CBP (CREB-binding protein), which may reprecent a CREB co-activator.

CREB-1 is recruited to and mediates upregulation of the cytochrome c promoter during enhanced mitochondrial biogenesis accompanying skeletal muscle differentiation . Mol. Cell. Biol., Apr 2008; 28: 2446 - 2459. il-10 regulation by hiv-tat in primary human monocytic cells: involvement of calmodulin/calmodulin-dependent protein kinase-activated p38 mapk and sp-1 and CREB-1 transcription factors . J. Immunol., Jan 2007; 178: 798 - 807. intracellular hiv-tat expression induces il-10 synthesis by the CREB-1 transcription factor through ser133 phosphorylation and its regulation by the erk1/2 mapk in human monocytic cells . J. Biol. Chem., Oct 2006; 281: 31647 - 31658. requirement of smad3 and CREB-1 in mediating transforming growth factor- (tgf) induction of tgf3 secretion . J. Biol. Chem., Oct 2006; 281: 29479 - 29490. impaired CREB-1 phosphorylation in antifolate-resistant cell lines with down-regulation of the reduced folate carrier gene . Mol. Pharmacol., Dec 2004; 66: 1536 - 1543.

4758054

NP_004370.1

NM_004379.3

P16220

36,688 Da

AKT Phosphorylates Targets In The Nucleus Pathway 106476!!ATF-2 Transcription Factor Network Pathway 138006!!Activated TLR4 Signalling Pathway 106400!!Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway 161033!!Adipogenesis Pathway 198832!!Antigen Processing And Presentation Pathway 83074!!Antigen Processing And Presentation Pathway 485!!Axon Guidance Pathway 105688!!B Cell Receptor Signaling Pathway 198909!!CREB Phosphorylation Pathway 106484

Function: Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells. Subunit structure: Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3. When phosphorylated on Ser-133, binds CREBBP . By similarity. Interacts with CREBL2; regulates CREB1 phosphorylation, stability and transcriptional activity . By similarity. Interacts (phosphorylated form) with TOX3. Interacts with ARRB1. Binds to HIPK2. Interacts with SGK1. Ref.9 Ref.10 Ref.15 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.26 Ref.27. Subcellular location: Nucleus Ref.16. Post-translational modification: Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding . By similarity. CREBL2 positively regulates phosphorylation at Ser-133 thereby stimulating CREB1 transcriptional activity . By similarity. Phosphorylated upon calcium influx by CaMK4 and CaMK2 on Ser-133. CaMK4 is much more potent than CaMK2 in activating CREB. Phosphorylated by CaMK2 on Ser-142. Phosphorylation of Ser-142 blocks CREB-mediated transcription even when Ser-133 is phosphorylated. Phosphorylated by CaMK1 . By similarity. Phosphorylation of Ser-271 by HIPK2 in response to genotoxic stress promotes CREB1 activity, facilitating the recruitment of the coactivator CBP. Phosphorylated at Ser-133 by RPS6KA3, RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli. Ref.11 Ref.12 Ref.13 Ref.14 Ref.20 Ref.26Sumoylated with SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Ref.16. Involvement in disease: Angiomatoid fibrous histiocytoma (AFH) [MIM:612160]: A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis.Note: The gene represented in this entry may be involved in disease pathogenesis. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.A CREB1 mutation has been found in a patient with multiple congenital anomalies consisting of agenesis of the corpus callosum, cerebellar hypoplasia, severe neonatal respiratory distress refractory to surfactant, thymus hypoplasia, and thyroid follicular hypoplasia (Ref.29). Sequence similarities: Belongs to the bZIP family.Contains 1 bZIP (basic-leucine zipper) domain.Contains 1 KID (kinase-inducible) domain.

0.1 mg

280 USD