Rabbit anti CD4 Polyclonal | MyBioSource MBS551067 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti CD4 Polyclonal

catalog :

MBS551067

quantity :

0.1 mg

price :

280 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS551067

products type :

Antibody

products full name :

Rabbit anti CD4 Polyclonal

products short name :

CD4

products name syn :

Rabbit anti CD4 Polyclonal purified; cd4; cd-4; polyclonal; PAb

other names :

T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain

products gene name :

CD4

products gene name syn :

CD4

other gene names :

CD3E; T3E

uniprot entry name :

CD3E_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

207

purity :

Serum IgG fraction was purified by Protein G affinity chromatography.

form :

Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.

storage stability :

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. Shelf Life: >12 months

tested application :

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

app notes :

ELISA: Use at a concentration range of 1-2ug/ml. WB: A suitable range of concentrations of this antibody for WB is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of FFPE human colorectal cancer sections showing lymphocyte expression of CD4. It was used as primary antibody, dilution 1:400, DAB chromogenic staining. Immunohistochemical analysis of FFPE mouse kidney sections showing lymphocyte expression of CD4. It was used as primary antibody, dilution 1:200, DAB chromogenic staining.

other info1 :

Source Note: Polyclonal CD4 antibody was produced from sera of rabbits immunized with synthetic protein containing CD4 antigenic determinants.

other info2 :

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers

products categories :

Polyclonal

products description :

CD4 is a 55 kDa transmembrane glycoprotein belonging to the immunoglobulin superfamily. In humans CD4 is expressed of peripheral T-cells, thymocytes, on macrophages and granulocytes. CD4 acts as a cellular adhesion molecule binding MHC class 2 molecules. It stabilizes the interaction of MHC class 2 restricted T- cells and antigen-presenting cells expressing an antigen in combination with a MHC class 2 molecule. Interactions of CD4 with MHC class 2 molecules are crucial during thymic development and subsequently for the function of single- positive CD4 (+), CD8 (-) T- lymphocytes. CD4 functions as an effector molecule for intracellular signal transduction. In T- lymphocytes CD4 has been found to be associated with tyrosine kinase lck which phosphorylates a number of other cell surface proteins and is involved probably in transducing the intracellular signal when these proteins are bound to their receptors.

products references :

hiv-infected ugandan adults taking antiretroviral therapy with CD4 counts 200 cells/ l who discontinue cotrimoxazole prophylaxis have increased risk of malaria and diarrhea . Clinical Infectious Diseases, Apr 2012; 54: 1204 - 1211. CD4 + type ii nkt cells mediate icos and programmed death-1 dependent regulation of type 1 diabetes . J. Immunol., Apr 2012; 188: 3138 - 3149. spatial alterations between CD4 + t follicular helper, b, and cd8+ t cells during simian immunodeficiency virus infection: t/b cell homeostasis, activation, and potential mechanism for viral escape . J. Immunol., Apr 2012; 188: 3247 - 3256. dissection of a circulating and intrahepatic CD4 +foxp3+ t-cell subpopulation in chronic hepatitis b virus (hbv) infection: a highly informative strategy for distinguishing chronic hbv infection states . The Journal of Infectious Disease, Apr 2012; 205: 1111 - 1120. mhc class ib-restricted cd8 t cells differ in dependence on CD4 t cell help and cd28 costimulation over the course of mouse polyomavirus infection . J. Immunol., Apr 2012; 188: 3071 - 3079.

ncbi gi num :

4502671

ncbi acc num :

NP_000724.1

ncbi gb acc num :

NM_000733.3

uniprot acc num :

P07766

ncbi mol weight :

23,147 Da

ncbi pathways :

Adaptive Immunity Signaling Pathway (366160); CXCR4-mediated Signaling Events Pathway (137910); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Costimulation By The CD28 Family Pathway (119552); Downstream TCR Signaling Pathway (106418); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); Generation Of Second Messenger Molecules Pathway (106417); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889)

ncbi summary :

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

uniprot summary :

CD3E: a T cell surface glycoprotein that is a component of the T cell antigen receptor. The recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Contains 1 immunoglobulin-like domain and 1 ITAM domain. Protein type: Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 11q23. Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; immunological synapse; alpha-beta T cell receptor complex; intercellular junction; external side of plasma membrane. Molecular Function: protein binding; transmembrane receptor activity; receptor signaling complex scaffold activity; protein heterodimerization activity; receptor signaling protein activity; T cell receptor binding; protein kinase binding; SH3 domain binding. Biological Process: regulation of immune response; T cell activation; positive regulation of interleukin-2 biosynthetic process; signal complex assembly; positive regulation of calcium-mediated signaling; T cell receptor signaling pathway; negative thymic T cell selection; positive regulation of interleukin-4 production; regulation of apoptosis; G-protein coupled receptor protein signaling pathway; positive regulation of interferon-gamma production; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; negative regulation of smoothened signaling pathway; T cell costimulation; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell proliferation; protein complex assembly; positive regulation of T cell anergy; transmembrane receptor protein tyrosine kinase signaling pathway; response to nutrient. Disease: Immunodeficiency 18

size1 :

0.1 mg

price1 :

280 USD

size2 :

0.2 mg

price2 :

385

more info or order :

MyBioSource product webpage