Rabbit anti BCL-2 Polyclonal | MyBioSource MBS551061 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti BCL-2 Polyclonal

catalog :

MBS551061

quantity :

0.1 mg

price :

280 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

WB analysis mouse hippocampal tissue lysate expressing BCL-2. MBS551061 was used as primary antibody.

image 2 :

Immunohistochemical analysis of PPFE rabbit hippocampal tissue expressing BCL-2. MBS551061 was used as primary antibody, dilution 1:200.

image 3 :

Immunohistochemical analysis of PPFE human endometrial tissue expressing BCL-2. MBS551061 was used as primary antibody, dilution 1:500.

product information

catalog number :

MBS551061

products type :

Antibody

products full name :

Rabbit anti BCL-2 Polyclonal

products short name :

[BCL-2]

products name syn :

[Rabbit anti BCL2 Polyclonal purified; BCL2; bcl-2; polyclonal]

other names :

[apoptosis regulator Bcl-2 alpha isoform; Apoptosis regulator Bcl-2]

products gene name :

[BCL-2]

other gene names :

[BCL2]

uniprot entry name :

BCL2_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

239

purity :

>95%

form :

Lyophilized from 0.2 um filtered solution in PBS pH 7.4

storage stability :

The lyophilized antibody is stable for at least 1 year from date of receipt at -20°C. Upon reconstitution, this antibody can be stored in working aliquots at 2-8°C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. Shelf Life: >12 months

tested application :

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

app notes :

ELISA (1-2ug/mL). , WB (2-10ug/mL). IHC (2-10ug/mL). Dilution: A quick spin of the vial followed by reconstitution in 0.01M PBS pH 7.4 or other diluents.

image1 heading :

Western blot

image2 heading :

Immunohistochemical

image3 heading :

Immunohistochemical

other info1 :

Source Note: Polyclonal BCL-2 antibody was produced from sera of mice immunized with synthetic protein containing BCL-2 antigenic determinants.

other info2 :

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers

products categories :

Polyclonal

products references :

akt activation emulates chk1 inhibition and BCL2 overexpression and abrogates g2 cell cycle checkpoint by inhibiting brca1 foci . J. Biol. Chem., May 2010; 10.1074/jbc.M110.104372. ifn restores breast cancer sensitivity to fulvestrant by regulating stat1, ifn regulatory factor 1, nf-b, BCL2 family members, and signaling to caspase-dependent apoptosis . Mol. Cancer Ther., May 2010; 9: 1274 - 1285. analysis of pro-apoptotic BCL2 family member initiators bim, bid, and bbc3 in retinal ganglion cell death . Invest. Ophthalmol. Vis. Sci., Apr 2010; 51: 2118. BCL2 and human papilloma virus 16 as predictors of outcome following concurrent chemoradiation for advanced oropharyngeal cancer . Clin. Cancer Res., Apr 2010; 16: 2138 - 2146. BCL2 is not required for the development and maintenance of leukaemia stem cells in mice . Carcinogenesis, Mar 2010; 10.1093/carcin/bgq062.

ncbi gi num :

72198189

ncbi acc num :

NP_000624.2

ncbi gb acc num :

NM_000633.2

uniprot acc num :

P10415

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); Activation Of BAD And Translocation To Mitochondria Pathway (105659); Activation Of BH3-only Proteins Pathway (105658); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (198797); Apoptosis Pathway (83060); Apoptosis Pathway (470); Apoptosis Pathway (105648); B Cell Receptor Signaling Pathway (198909)

ncbi summary :

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]

uniprot summary :

Bcl-2: a antiapoptotic member of the Bcl-2 family. Regulates cell death by controlling the mitochondrial membrane permeability. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Phosphorylation by JNKs may increase its antiapoptotic functions. Protein type: Oncoprotein; Membrane protein, integral; Apoptosis; Autophagy. Chromosomal Location of Human Ortholog: 18q21.3. Cellular Component: pore complex; mitochondrial outer membrane; endoplasmic reticulum membrane; nuclear membrane; mitochondrion; membrane; endoplasmic reticulum; cytoplasm; cytosol; nucleus; myelin sheath. Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protease binding; protein phosphatase 2A binding; sequence-specific DNA binding; protein heterodimerization activity; channel activity; ubiquitin protein ligase binding; BH3 domain binding; channel inhibitor activity; transcription factor binding. Biological Process: focal adhesion formation; positive regulation of catalytic activity; response to nicotine; developmental growth; renal system process; pigment granule organization and biogenesis; protein polyubiquitination; response to toxin; response to glucocorticoid stimulus; T cell differentiation in the thymus; ear development; lymphoid progenitor cell differentiation; female pregnancy; positive regulation of multicellular organism growth; glomerulus development; negative regulation of mitochondrial depolarization; post-embryonic development; cochlear nucleus development; cellular response to glucose starvation; B cell receptor signaling pathway; negative regulation of myeloid cell apoptosis; regulation of mitochondrial membrane potential; positive regulation of B cell proliferation; negative regulation of ossification; regulation of transmembrane transporter activity; T cell homeostasis; negative regulation of neuron apoptosis; cell growth; defense response to virus; spleen development; response to drug; positive regulation of neuron maturation; release of cytochrome c from mitochondria; axon regeneration; regulation of protein homodimerization activity; actin filament organization; cell aging; digestive tract morphogenesis; regulation of calcium ion transport; positive regulation of cell growth; organ growth; induction of apoptosis via death domain receptors; DNA damage response, signal transduction resulting in induction of apoptosis; negative regulation of osteoblast proliferation; gland morphogenesis; regulation of mitochondrial membrane permeability; regulation of nitrogen utilization; metanephros development; oocyte development; negative regulation of apoptosis; B cell proliferation; negative regulation of autophagy; regulation of protein heterodimerization activity; behavioral fear response; melanin metabolic process; negative regulation of retinal cell programmed cell death; regulation of cell-matrix adhesion; apoptosis; regulation of protein stability; positive regulation of smooth muscle cell migration; protein amino acid dephosphorylation; response to radiation; B cell homeostasis; ovarian follicle development; positive regulation of skeletal muscle fiber development; positive regulation of melanocyte differentiation; melanocyte differentiation; response to gamma radiation; negative regulation of cell migration; regulation of viral genome replication; transmembrane transport; response to iron ion; negative regulation of cellular pH reduction; mesenchymal cell development; ossification; hair follicle morphogenesis; CD8-positive, alpha-beta T cell lineage commitment; thymus development; B cell lineage commitment; male gonad development; peptidyl-threonine phosphorylation; positive regulation of peptidyl-serine phosphorylation; humoral immune response; response to UV-B; endoplasmic reticulum calcium ion homeostasis; peptidyl-serine phosphorylation; neuron apoptosis; axonogenesis; response to hydrogen peroxide; ureteric bud branching; homeostasis of number of cells within a tissue; response to cytokine stimulus; innate immune response; negative regulation of cell growth; response to DNA damage stimulus; induction of apoptosis by oxidative stress

size1 :

0.1 mg

price1 :

280 USD

size2 :

0.2 mg

price2 :

385

more info or order :

MyBioSource product webpage