Rabbit anti Osteopontin Polyclonal | MyBioSource MBS551057 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti Osteopontin Polyclonal

catalog :

MBS551057

quantity :

0.1 mg

price :

280 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS551057

products type :

Antibody

products full name :

Rabbit anti Osteopontin Polyclonal

products short name :

Osteopontin

products name syn :

Rabbit anti Osteopontin Polyclonal purified; SPP1; OPN; osteopontin; polyclonal

other names :

osteopontin isoform OPN-b; Osteopontin; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin

products gene name :

OPN

other gene names :

SPP1; BNSP; OPN; SPP-1

uniprot entry name :

OSTP_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mouse, Rat

sequence length :

300

purity :

Serum IgG fraction was purified by Protein G affinity chromatography. > 95%

form :

Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.

storage stability :

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. Shelf Life: >12 months

tested application :

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

app notes :

ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of FFPE mouse Thyroid sections expressing Osteopontin. It was used as primary antibody, dilution 1:500, DAB staining.

other info1 :

Source Note: Polyclonal anti Osteopontin (OPN) antibody was produced from sera of rabbits immunized with synthetic protein containing Ostepontin antigenic determinants.

other info2 :

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers

products categories :

Polyclonal

products references :

differential expression of intracellular and secreted Osteopontin isoforms by murine macrophages in response to toll-like receptor agonists . J. Biol. Chem., Jul 2010; 285: 20452 - 20461. inhibitory action of wnt target gene Osteopontin on mitochondrial cytochrome c release determines renal ischemic resistance . Am J Physiol Renal Physiol, Jul 2010; 299: F234 - F242. changes of Osteopontin (opn) in the aqueous humor of the dba2/j glaucoma model correlated with optic nerve and rgc degenerations . Invest. Ophthalmol. Vis. Sci., Jun 2010; 10.1167/iovs.10-5558. identification of an il-27/ Osteopontin axis in dendritic cells and its modulation by ifn- limits il-17 mediated autoimmune inflammation . PNAS, Jun 2010; 107: 11495 - 11500. Osteopontin expression and relation to disease severity in human asthma . Eur. Respir. J., Jun 2010; 10.1183/09031936.00017810.

ncbi gi num :

4759166

ncbi acc num :

NP_000573.1

ncbi gb acc num :

NM_000582.2

uniprot acc num :

P10451

ncbi mol weight :

35,423 Da

ncbi pathways :

Direct P53 Effectors Pathway (137939); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Endochondral Ossification Pathway (198812); FGF Signaling Pathway (137989); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Integrin Cell Surface Interactions Pathway (106110); Integrins In Angiogenesis Pathway (137999)

ncbi summary :

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

uniprot summary :

osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q22.1. Cellular Component: extracellular space; cell projection; perinuclear region of cytoplasm; apical part of cell; extracellular region. Molecular Function: protein binding; extracellular matrix binding; cytokine activity. Biological Process: osteoblast differentiation; extracellular matrix disassembly; neutrophil chemotaxis; extracellular matrix organization and biogenesis; response to vitamin D; response to steroid hormone stimulus; biomineral formation; negative regulation of collateral sprouting of intact axon in response to injury; positive regulation of bone resorption; decidualization; inflammatory response; cell adhesion; embryo implantation

size1 :

0.1 mg

price1 :

280 USD

size2 :

0.2 mg

price2 :

385

more info or order :

MyBioSource product webpage