Rabbit anti IL-1 beta Polyclonal | MyBioSource MBS551056 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti IL-1 beta Polyclonal

catalog :

MBS551056

quantity :

0.1 mg

price :

280 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS551056

products type :

Antibody

products full name :

Rabbit anti IL-1 beta Polyclonal

products short name :

IL-1 beta

products name syn :

Rabbit anti IL-1 beta Polyclonal purified; interleukin-1; hbgf; heparin binding; il1b; il-1 beta; il1-beta; polyclonal; pab

other names :

interleukin-1 beta proprotein; Interleukin-1 beta; Catabolin

products gene name :

IL-1

other gene names :

IL1B; IL1F2; IL-1 beta

uniprot entry name :

IL1B_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

269

purity :

Serum IgG fraction was purified by Protein G affinity chromatography. Purity: >95%

form :

Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.

storage stability :

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. Shelf Life: >12 months

tested application :

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

app notes :

ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.

other info1 :

Source Note: Polyclonal anti IL-1b was produced from sera of rabbits immunized with synthetic protein containing Interleukin-1 beta antigenic determinants. Presentation: Lyophilized from 0.2um filtered solution in PBS pH 7.4.

other info2 :

Dilution: A quick spin of the vial followed by reconstitution in 0.01M PBS pH 7.4 or other diluents.

products categories :

Polyclonal

products description :

Monocytes are the main source of secreted IL1. They express predominantly IL1- beta while human keratinocytes express large amounts of IL1-alpha. Murine macrophages display a transition from IL1-beta to IL1-alpha production during maturation of monocytes into inflammatory macrophages.There are two functionally almost equivalent forms of IL1, IL1-alpha and IL1-beta that are encoded by two different genes. IL1-beta is the predominant form in humans while it is IL1-alpha in mice. Both forms of IL1 bind to the same receptor and therefore also show similar if not identical biological activities. The IL1-beta but not the IL1-alpha precursor must be processed before it can bind to the receptor. Both forms of IL1 bind to the same receptor and therefore also show similar if not identical biological activities. The receptor isolated from T-cells is expressed predominantly on T-cells and cells of mesenchymal origin. It binds both types of IL1 with equal affinity. This type is called also Type 1 receptor. It has been designated CD121a. The Type 2 receptor has been designated CD121b. It is isolated from B-cells, granulocytes, and macrophages. It is expressed predominantly on B-cells and cells of the myelomonocytic lineage and is encoded by a separate gene.

products references :

azithromycin and clarithromycin inhibit lipopolysaccharide-induced murine pulmonary neutrophilia mainly through effects on macrophage-derived granulocyte-macrophage colony-stimulating factor and interleukin-1 . J. Pharmacol. Exp. Ther., Oct 2009; 331: 104 - 113. high sensitivity of -cell replication to the inhibitory effects of interleukin-1 : modulation by adenoviral overexpression of igf2 in rat islets . J. Endocrinol., Oct 2009; 203: 55 - 63. bacillus anthracis capsule activates caspase-1/ice and induces interleukin-1 release from differentiated thp-1 and human monocyte-derived dendritic cells. . Infect. Immun., Sep 2009; 10.1128/IAI.00956-09. Interleukin-1 beta regulates proximal tubular cell transforming growth factor beta-1 signalling . Nephrol. Dial. Transplant., Sep 2009; 24: 2655 - 2665. ccaat enhancer binding protein- regulates matrix metalloproteinase-1 expression in interleukin-1 stimulated a549 lung carcinoma cells . Mol. Cancer Res., Sep 2009; 7: 1517 - 1524.

ncbi gi num :

10835145

ncbi acc num :

NP_000567.1

ncbi gb acc num :

NM_000576.2

uniprot acc num :

P01584

ncbi mol weight :

30,748 Da

ncbi pathways :

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Apoptosis Pathway (83060); Apoptosis Pathway (470); Cellular Roles Of Anthrax Toxin Pathway (138076); Chagas Disease (American Trypanosomiasis) Pathway (147809)

ncbi summary :

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]

uniprot summary :

IL1B: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Monomer. Belongs to the IL-1 family. Protein type: Cytokine. Chromosomal Location of Human Ortholog: 2q14. Cellular Component: extracellular space; extracellular region; cytosol; secretory granule. Molecular Function: protein domain specific binding; interleukin-1 receptor binding; cytokine activity. Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; negative regulation of MAP kinase activity; positive regulation of nitric oxide biosynthetic process; activation of MAPK activity; positive regulation of interleukin-2 biosynthetic process; positive regulation of transcription, DNA-dependent; germ cell programmed cell death; negative regulation of insulin receptor signaling pathway; positive regulation of NF-kappaB import into nucleus; positive regulation of lipid catabolic process; fever; positive regulation of membrane protein ectodomain proteolysis; response to carbohydrate stimulus; activation of NF-kappaB transcription factor; cell-cell signaling; positive regulation of phagocytosis; positive regulation of T cell mediated immunity; positive regulation of T cell proliferation; neutrophil chemotaxis; positive regulation of heterotypic cell-cell adhesion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of mitosis; smooth muscle adaptation; interleukin-1 beta production; positive regulation of interleukin-6 production; positive regulation of angiogenesis; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of lipid metabolic process; sequestering of triacylglycerol; positive regulation of histone phosphorylation; apoptosis; positive regulation of interleukin-6 biosynthetic process; positive regulation of JNK cascade; signal transduction; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of interleukin-8 production; positive regulation of protein export from nucleus; negative regulation of cell proliferation; negative regulation of lipid catabolic process; hyaluronan biosynthetic process; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; inflammatory response; regulation of I-kappaB kinase/NF-kappaB cascade; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; response to ATP; positive regulation of interferon-gamma production; positive regulation of chemokine biosynthetic process; positive regulation of prostaglandin secretion; positive regulation of fever; positive regulation of histone acetylation; immune response; positive regulation of protein amino acid phosphorylation; regulation of insulin secretion; embryo implantation. Disease: Gastric Cancer, Hereditary Diffuse

size1 :

0.1 mg

price1 :

280 USD

size2 :

0.2 mg

price2 :

385

more info or order :

MyBioSource product webpage