antibody

Rabbit anti IFN-g Polyclonal

MBS551054

0.1 mg

280 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Rabbit anti IFN-g Polyclonal

IFN-g

Rabbit anti Human IFN-g Polyclonal purified; ifn-gamma; ifng; pab; polyclonal

interferon gamma; Interferon gamma; Immune interferon

IFN-g

IFN-gamma

IFNG; IFN-gamma

IFNG_HUMAN

Polyclonal

IgG

Rabbit

Mouse, Rat

166

>95 %

Lyophilized from .2um filtered solution in PBS pH 7.4 or other diluents.

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. Shelf Life: >12 months

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

ELISA: Use at a concentration range of 1-2ug/ml. Western blot: A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC-F/P: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of increased IFN-gamma expression in the mucosal surface of rat intestinal tissue. It was used as primary antibody, DAB chromogen, dilution 1:200.

Source Note: Polyclonal Interferon-gamma antibody was produced from sera of rabbits immunized with synthetic protein containing IFN-gamma antigenic determinants.

Reconstitution: A quick spin of the vial followed by reconstitution in 0.01M PBS pH 7.4 or other diluents. Presentation: Lyophilized from 0.2um filtered solution in PBS pH 7.4. Purification: Serum IgG fragtion was purified by Protein G affinity chromatography.

Polyclonal

IFN-gamma is produced mainly by T-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFN gamma receptors are expressed on all types of human cells with the exception of mature erythrocytes.IFN-gamma- receptor complexes are rapidly internalized by endocytosis. IFN-gamma has antiviral and antiparasitic activities and also inhibits the proliferation of a number of normal and transformed cells. Interferon-gamma synergises with TNF-alpha and TNF-beta in inhibiting the proliferation of various cell types. The growth inhibitory activities of IFN-gamma are more pronounced than those of the other interferons. However, the main biological activity of IFN-gamma appears to be immunomodulatory in contrast to the other interferons that are mainly antiviral. n T-helper cells IL2 induces the synthesis of IFN-gamma and other cytokines. IFN- gamma acts synergistically with IL1 and IL2 and appears to be required for the expression of IL2 receptors on the cell surface of T- lymphocytes. Blocking of the IL2 receptor by specific antibodies also inhibits the synthesis of IFN-gamma.

diminished frequency of nk cells and cd4+ IFN-g + t cells in patients with mycotic corneal ulcers . Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 1945. IFN-g -stimulated mesenchymal stromal cells acquire the ability to cross-present exogenous soluble antigens and lead to an effective immune response. . Blood (ASH Annual Meeting Abstracts), Nov 2007; 110: 4898. regulation of tlr expression and activation in human mesenchymal stromal cells by ifn-a, IFN-g and tnf-a. . Blood (ASH Annual Meeting Abstracts), Nov 2007; 110: 1790. positive association of tnf-857 and ifn g 874 gene polymorphisms with chronic fatigue syndrome . Ann Rheum Dis, Jun 2005; 64: 344. measurement of functional cytomegalovirus (cmv) -specific t cells by interferon-gamma-enzyme-linked immunospot ( IFN-g elispot) assay specific for a single immunodominant epitope enables the sensitive evaluation of immune recovery for cmv in patients after allogeneic stem cell transplantation (hsct) with a reduced-intensity regimen (rist). . Blood (ASH Annual Meeting Abstracts), Nov 2004; 104: 3157.

56786138

NP_000610.2

NM_000619.2

P01579

19,348 Da

ATF-2 Transcription Factor Network Pathway (138006); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993)

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015]

IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family. Protein type: Cytokine; Membrane protein, integral; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 12q14. Cellular Component: extracellular space; extracellular region; external side of plasma membrane. Molecular Function: interferon-gamma receptor binding; cytokine activity. Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; apoptosis; negative regulation of smooth muscle cell proliferation; positive regulation of osteoclast differentiation; positive regulation of interleukin-12 production; positive regulation of interleukin-23 production; positive regulation of interleukin-6 biosynthetic process; negative regulation of epithelial cell differentiation; positive regulation of interleukin-1 beta secretion; negative regulation of transcription from RNA polymerase II promoter; sensory perception of mechanical stimulus; positive regulation of killing of cells of another organism; positive regulation of membrane protein ectodomain proteolysis; cell surface receptor linked signal transduction; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to drug; positive regulation of synaptic transmission, cholinergic; neutrophil chemotaxis; adaptive immune response; CD8-positive, alpha-beta T cell differentiation during immune response; negative regulation of myelination; unfolded protein response; response to virus; cytokine and chemokine mediated signaling pathway; positive regulation of tumor necrosis factor production; defense response to protozoan; humoral immune response; antigen processing and presentation; positive regulation of tyrosine phosphorylation of Stat1 protein; negative regulation of interleukin-17 production; positive regulation of chemokine biosynthetic process; positive regulation of interleukin-12 biosynthetic process; protein import into nucleus, translocation; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; cell motility; positive regulation of neuron differentiation; regulation of insulin secretion. Disease: Hepatitis C Virus, Susceptibility To; Aplastic Anemia; Mycobacterium Tuberculosis, Susceptibility To; Tuberous Sclerosis 2; Human Immunodeficiency Virus Type 1, Susceptibility To

0.1 mg

280 USD

0.2 mg

385