Rabbit anti MCP-1 Polyclonal | MyBioSource MBS551047 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti MCP-1 Polyclonal

catalog :

MBS551047

quantity :

0.1 mg

price :

280 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:400	Chen R, Zhang F, Song L, Shu Y, Lin Y, Dong L, et al. Transcriptome profiling reveals that the SM22?-regulated molecular pathways contribute to vascular pathology. J Mol Cell Cardiol. 2014;72:263-72 pubmed publisher

product information

catalog number :

MBS551047

products type :

Antibody

products full name :

Rabbit anti MCP-1 Polyclonal

products short name :

MCP-1

products name syn :

Rabbit anti MCP-1 Polyclonal purified; mcp-1; mcp1; ccl2; ccl-2; pab; polyclonal

other names :

C-C motif chemokine 2; C-C motif chemokine 2; HC11; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; MCP-1; Monocyte secretory protein JE; Small-inducible cytokine A2

products gene name :

MCP-1

other gene names :

CCL2; MCP1; SCYA2; MCAF; MCP-1

uniprot entry name :

CCL2_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

purity :

>95%. Purification: . Serum IgG fraction was purified by Protein G affinity chromatography.

form :

Lyophilized from 0.2um filtered solution in PBS pH 7.4

storage stability :

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. Shelf Life: >12 months

tested application :

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

app notes :

ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of MCP-1 expression in paraffin fixed obese mouse proximal tubule tissue. It was used as primary antibody, dilution 1:400.

other info1 :

Source Note: Polyclonal MCP-1 (CCL2) antibody was produced from sera of rabbits immunized with synthetic protein containing MCP-1 antigenic determinants.

other info2 :

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers. Dilution: A quick spin of the vial followed by reconsititution in 0.01M PBS pH 7.4 or other diluents.

products categories :

Polyclonal

products description :

MCP-1 belongs to the family of chemotactic cytokines.MCP-1 is expressed by monocytes, vascular endothelial cells, smooth muscle cells, glomerular mesangial cell, osteoblastic cells. MCP-1 has been shown to exhibit biological activities other than Chemotaxis. It can induce the proliferation and activation of killer cells known as CHAK. Elevated levels of MCP-1 are observed in atherosclerotic plaques that are rich in macrophages. The factor activates the tumoricidal activity of monocytes and macrophages in vivo. It regulates the expression of cell surface antigens (CD11c, CD11b) and the expression of cytokines (IL1, IL6). MCP-1 is a potent activator of human basophils, inducing degranulation and the release of histamines. In basophils activated by IL3, IL5 or GM-CSF MCP-1 enhances the synthesis of leukotriene C4.

products references :

rationally evolving mcp-1 /ccl2 into a decoy protein with potent anti-inflammatory activity in vivo . J. Biol. Chem., Mar 2010; 285: 8782 - 8792. association of the mcp-1 -2518 a/g polymorphism and no association of its receptor ccr2 -64 v/i polymorphism with lupus nephritis . J Rheumatol, Mar 2010; 10.3899/jrheum.090681. mcp-1 /ccl2 modifies axon properties in a pmp22-overexpressing mouse model for charcot-marie-tooth 1a neuropathy . Am. J. Pathol., Mar 2010; 176: 1390 - 1399. critical protective role for mcp-1 in pneumonic burkholderia mallei infection . J. Immunol., Feb 2010; 184: 1445 - 1454. thioredoxin 1 downregulates mcp-1 secretion in human endothelial cells by suppressing nuclear translocation of activator protein 1 and redox factor-1 . Am J Physiol Cell Physiol, Dec 2009; 10.1152/ajpcell.00223.2009.

ncbi gi num :

4506841

ncbi acc num :

NP_002973.1

ncbi gb acc num :

NM_002982.3

uniprot acc num :

P13500

ncbi mol weight :

11,025 Da

ncbi pathways :

Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Diabetes Pathways (105902); GPCR Ligand Binding Pathway (161020)

ncbi summary :

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]

uniprot summary :

CCL2: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Belongs to the intercrine beta (chemokine CC) family. Protein type: Chemokine; Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 17q11.2-q12. Cellular Component: extracellular space; rough endoplasmic reticulum; perinuclear region of cytoplasm; endocytic vesicle; dendrite; extracellular region; synapse; perikaryon; nerve terminal. Molecular Function: heparin binding; CCR2 chemokine receptor binding; chemokine activity; receptor binding; protein kinase activity. Biological Process: maternal process involved in pregnancy; protein amino acid phosphorylation; response to antibiotic; regulation of cell shape; monocyte chemotaxis; cell surface receptor linked signal transduction; transforming growth factor beta receptor signaling pathway; response to vitamin B3; negative regulation of neuron apoptosis; cellular homeostasis; cell adhesion; neutrophil chemotaxis; organ regeneration; JAK-STAT cascade; positive regulation of tumor necrosis factor production; response to amino acid stimulus; G-protein signaling, coupled to cyclic nucleotide second messenger; organ morphogenesis; response to ethanol; unfolded protein response, activation of signaling protein activity; cellular response to insulin stimulus; response to bacterium; response to mechanical stimulus; response to heat; positive regulation of endothelial cell proliferation; response to activity; response to progesterone stimulus; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of collagen biosynthetic process; signal transduction; chemotaxis; positive regulation of synaptic transmission; positive regulation of cellular extravasation; response to wounding; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; response to gamma radiation; angiogenesis; inflammatory response; lymphocyte chemotaxis; aging; unfolded protein response; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; cytoskeleton organization and biogenesis; viral genome replication; macrophage chemotaxis; leukocyte migration during inflammatory response; humoral immune response; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; positive regulation of leukocyte mediated cytotoxicity; negative regulation of angiogenesis; cellular protein metabolic process; maternal process involved in parturition; response to hypoxia; positive regulation of T cell activation; vascular endothelial growth factor receptor signaling pathway; astrocyte cell migration. Disease: Neural Tube Defects; Mycobacterium Tuberculosis, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To

size1 :

0.1 mg

price1 :

280 USD

size2 :

0.2 mg

price2 :

385

more info or order :

MyBioSource product webpage