Rabbit anti bFGF Polyclonal | MyBioSource MBS551041 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti bFGF Polyclonal

catalog :

MBS551041

quantity :

0.1 mg

price :

280 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS551041

products type :

Antibody

products full name :

Rabbit anti bFGF Polyclonal

products short name :

bFGF

products name syn :

Rabbit anti bFGF Polyclonal purified; fgf-2; fibrobalst growth factor; fgf2; bfgf; polyclonal; pab

other names :

fibroblast growth factor 2; Fibroblast growth factor 2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2

products gene name :

bFGF

other gene names :

FGF2; FGFB; FGF-2; bFGF; HBGF-2

uniprot entry name :

FGF2_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

288

purity :

Serum IgG fraction was purified by Protein G affinity chromatography.

form :

Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.

storage stability :

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. Shelf Life: >12 months

tested application :

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

app notes :

ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.

other info1 :

Source Note: Polyclonal FGF basic antibody was produced from sera of rabbits immunized with synthetic protein containing FGF basic antigenic determinants.

other info2 :

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers

products categories :

Polyclonal

products description :

basic Fibroblast Growth Factor is found in almost all tissues of mesodermal and neuroectodermal origin and also in tumors derived from these tissues. Endothelial cells produce large amounts of this factor. Some bFGF is associated with the extracellular matrix of the subendothelial cells. Many cells express bFGF only transiently and store it in a biologically inactive form. The mechanism by which the factor is released by the cells is not known. It is released after tissue injuries and during inflammatory processes. FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation. bFGF stimulates the growth of fibroblasts, myoblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, chondrocytes, and many other cell types.

ncbi gi num :

153285461

ncbi acc num :

NP_001997.5

ncbi gb acc num :

NM_002006.4

uniprot acc num :

P09038

ncbi mol weight :

30,770 Da

ncbi pathways :

Angiopoietin Receptor Tie2-mediated Signaling Pathway 137917!!Downstream Signaling Of Activated FGFR Pathway 160957!!Endochondral Ossification Pathway 198812!!FGFR Ligand Binding And Activation Pathway 106344!!FGFR1 Ligand Binding And Activation Pathway 106345!!FGFR1b Ligand Binding And Activation Pathway 106346!!FGFR1c Ligand Binding And Activation Pathway 106347!!FGFR2 Ligand Binding And Activation Pathway 106349!!FGFR2c Ligand Binding And Activation Pathway 106351!!FGFR3 Ligand Binding And Activation Pathway 106352

uniprot summary :

Function: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Ref.11 Ref.20. Subunit structure: Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24 Ref.25. Subcellular location: Secreted. Nucleus. Note: Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57. Ref.25 Ref.28. Tissue specificity: Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue. Ref.11 Ref.18. Post-translational modification: Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. Miscellaneous: This protein binds heparin more strongly than does aFGF. Sequence similarities: Belongs to the heparin-binding growth factors family. Biophysicochemical propertiespH dependence:Retains almost half of its activity after treatment at pH 2.0 for 3 hours at 20 degrees Celsius. Ref.11Temperature dependence:Inactivated after 3 minutes at 60 degrees Celsius or 1 minute at 80 degrees Celsius. Sequence caution: The sequence AAA52448.1 differs from that shown. Reason: Frameshift at positions 25, 82, 98 and 133. The sequence AAB21432.2 differs from that shown. Reason: Frameshift at position 25. The sequence AAB21432.2 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.The sequence ABO43041.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence ABO43041.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.The sequence CAA28027.1 differs from that shown. Reason: Frameshift at positions 25 and 102. The sequence CAA28027.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.The sequence CAA73868.1 differs from that shown. Reason: Frameshift at position 25. The sequence CAA73868.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.The sequence EAX05222.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence EAX05222.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.

size :

0.1 mg

price :

280 USD

more info or order :

MyBioSource product webpage