Rabbit anti CD34 Polyclonal | MyBioSource MBS551039 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti CD34 Polyclonal

catalog :

MBS551039

quantity :

0.1 mg

price :

280 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS551039

products type :

Antibody

products full name :

Rabbit anti CD34 Polyclonal

products short name :

CD34

products name syn :

Rabbit anti CD34 Polyclonal purified; pecam1; pecam-1; cd34; cd-34; polyclonal; PAb

other names :

hematopoietic progenitor cell antigen CD34 isoform a; Hematopoietic progenitor cell antigen CD34

products gene name :

CD34

other gene names :

CD34

uniprot entry name :

CD34_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

385

purity :

>95%

form :

Lyophilized from 0.2 um filtered solution in PBS pH 7.4

storage stability :

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. Shelf Life: >12 months

tested application :

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

app notes :

ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.

other info1 :

Source Note: Polyclonal CD34 antibody was produced from sera of rabbits immunized with synthetic protein containing CD34 antigenic determinants.

other info2 :

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers

products categories :

Polyclonal

products description :

The membrane glycoprotein CD34 has been proven to be a useful marker for human hematopoietic stem/progenitor cells, and various types of colony- forming activity in human bone marrow, cord blood, and mobilized peripheral blood are contained in the CD34+ population.

products references :

ordering human CD34 +cd10 cd19+ pre/pro-b-cell and cd19 common lymphoid progenitor stages in two pro-b-cell development pathways . PNAS, Mar 2010; 10.1073/pnas.0907942107. leukemia-initiating cells from some acute myeloid leukemia patients with mutated nucleophosmin reside in the CD34 fraction . Blood, Mar 2010; 115: 1976 - 1984. transient -catenin stabilization modifies lineage output from human thymic CD34 +cd1a progenitors . J. Leukoc. Biol., Mar 2010; 87: 405 - 414. association of hla-a, -b and -drb1 genotype with birth-weight and CD34 + cell content; analysis of korean newborns and their cord blood . Mol. Hum. Reprod., Feb 2010; 10.1093/molehr/gaq011. purified t-depleted, CD34 + peripheral blood and bone marrow cell transplantation from haploidentical mother to child with thalassemia . Blood, Feb 2010; 115: 1296 - 1302.

ncbi gi num :

68342038

ncbi acc num :

NP_001020280.1

ncbi gb acc num :

NM_001025109.1

uniprot acc num :

P28906

ncbi mol weight :

40,716 Da

ncbi pathways :

Adaptive Immunity Signaling Pathway (366160); C-MYB Transcription Factor Network Pathway (138073); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Immune System Pathway (106386); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (106413)

ncbi summary :

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

uniprot summary :

CD34: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. Belongs to the CD34 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell surface; Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q32. Cellular Component: intercellular bridge; lysosome; perinuclear region of cytoplasm; integral to plasma membrane; apical plasma membrane; cytoplasm; plasma membrane; basal plasma membrane; external side of plasma membrane. Molecular Function: sulfate binding; carbohydrate binding; transcription factor binding. Biological Process: positive regulation of odontogenesis; regulation of immune response; negative regulation of nitric oxide biosynthetic process; glomerular filtration; cell motility involved in cell locomotion; negative regulation of blood coagulation; negative regulation of tumor necrosis factor production; signal transduction; positive regulation of interleukin-10 production; cell proliferation; cell-cell adhesion; positive regulation of angiogenesis; regulation of blood pressure; endothelial cell proliferation; tissue homeostasis; hemopoiesis; leukocyte migration

size1 :

0.1 mg

price1 :

280 USD

size2 :

0.2 mg

price2 :

385

more info or order :

MyBioSource product webpage