Rabbit anti CD31 (PECAM1) Polyclonal | MyBioSource MBS551038 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti CD31 (PECAM1) Polyclonal

catalog :

MBS551038

quantity :

0.1 mg

price :

280 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS551038

products type :

Antibody

products full name :

Rabbit anti CD31 (PECAM1) Polyclonal

products short name :

CD31 (PECAM1)

products name syn :

Rabbit anti CD31 Polyclonal purified; pecam1; pecam-1; cd31; cd-31; polyclonal; PAb

other names :

platelet endothelial cell adhesion molecule; Platelet endothelial cell adhesion molecule; EndoCAM; GPIIA'; PECA1

products gene name :

CD31

other gene names :

PECAM1; PECAM-1

uniprot entry name :

PECA1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

738

purity :

>95%

form :

Lyophilized from 0.2um filtered solution in PBS pH7.4.

storage stability :

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. Shelf Life: >12 months

tested application :

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

app notes :

ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of CD31-positive microvessels in FFPE Human malignant melanoma cell line, A375. It was used as primary antibody, dilution 1:200, DAB chromogen substrate. Immunohistochemical analysis of mouse CD31 expression in FFPE vascular endothelial melanoma cell. It was used as primary antibody, dilution 1:200, DAB chromogen substrate.

other info1 :

Source Note: PolyclonalCD31 antibody was produced from sera of rabbits immunized with synthetic protein containing CD31 antigenic determinants.

other info2 :

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers. Dilution: a quick spin of the vial followed by reconstitution in 0.01M PBS pH 7.4 or other diluents.

products categories :

Polyclonal

products description :

Platelet endothelial cell adhesion molecule (PECAM-1, CD31) is a 130 kD glycoprotein that is constitutively expressed by endothelial cells and by platelets, neutrophils, monocytes, and some T- lymphocytes. It is an integral transmembrane protein that belongs to the immunoglobulin superfamily and that mediates both homotypic and heterotypic cell adhesion. These functions have implicated CD31 in a variety of important biological processes including inflammation, angiogenesis and wound healing.In particular, CD31 has an important role in neutrophil and monocyte emigration across vascular endothelium, processes that can be inhibited, both in vitro and in vivo, with anti-CD31 antibodies.

products references :

prognostic and predictive impact of expression of the angiogenic markers cd105 and CD31 expression in patients with high risk breast cancer (hrbc). . Cancer Res., Dec 2009; 69: 2154. cd38/ CD31 , the ccl3 and ccl4 chemokines, and cd49d/vascular cell adhesion molecule-1 are interchained by sequential events sustaining chronic lymphocytic leukemia cell survival . Cancer Res., May 2009; 69: 4001 - 4009. circulating monocytes expressing CD31 : implications for acute and chronic angiogenesis . Am. J. Pathol., May 2009; 174: 1972 - 1980. il-7 sustains CD31 expression in human naive cd4+ t cells and preferentially expands the CD31 + subset in a pi3k-dependent manner . Blood, Mar 2009; 113: 2999 - 3007. transcriptional profiling of CD31 + cells isolated from murine embryonic stem cells . Genes Cells, Feb 2009; 14: 243 - 260.

ncbi gi num :

313760624

ncbi acc num :

NP_000433.4

ncbi gb acc num :

NM_000442.4

uniprot acc num :

P16284

ncbi pathways :

Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (106062); Formation Of Platelet Plug Pathway (106029); Hemostasis Pathway (106028); Integrin Cell Surface Interactions Pathway (106110); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494); Malaria Pathway (152665); Malaria Pathway (152657)

ncbi summary :

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

uniprot summary :

PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues. Protein type: Cell adhesion; Membrane protein, integral. Chromosomal Location of Human Ortholog: 17q23.3. Cellular Component: extracellular space; platelet alpha granule membrane; plasma membrane; integral to membrane; cell junction. Molecular Function: protein binding. Biological Process: platelet activation; extracellular matrix organization and biogenesis; platelet degranulation; diapedesis; cell recognition; signal transduction; blood coagulation; cell adhesion; leukocyte migration; phagocytosis

size1 :

0.1 mg

price1 :

280 USD

size2 :

0.2 mg

price2 :

385

more info or order :

MyBioSource product webpage