antibody

Rabbit anti M-CSF Polyclonal

MBS551037

0.1 mg

280 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Rabbit anti M-CSF Polyclonal

M-CSF

Rabbit anti M-CSF Polyclonal purified; CSF-1; csf1; m-csf; macrophage colony stimulting; mcsf; polyclonal; PAb

macrophage colony-stimulating factor 1 isoform a; Macrophage colony-stimulating factor 1; Lanimostim

M-CSF

CSF1; CSF-1; M-CSF; MCSF

CSF1_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

554

>95%. Purification: Serum IgG fraction was purified by Protein G affinity chromatography.

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. Shelf Life: >12 months

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

ELISA: Use at a concentration range of 1-2ug/ml. Western blot: A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.

Source Note: Polyclonal M-CSF antibody was produced from sera of rabbits immunized with synthetic protein containing M-CSF antigenic determinants.

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers. Presentation: 0.2um filtered 1mg/ml solution in TBS pH 7.4, 50% glycerol, 1% BSA, 0.03% proclin. Dilution: A quick spin of the vial followed by reconstitution in 0.01M PBS pH 7.4 or other diluents.

Polyclonal

M-CSF is produced by monocytes, granulocytes, endothelial cells, and fibroblasts. After cell activation, B-cells and T- cells and also a number of tumor cell lines are capable also of synthesizing this factor. M-CSF has been found to be synthesized by uterine epithelial cells in vivo. The M-CSF receptor is identical with the proto- oncogene fms. It has been renamed CD115. The receptor is a transmembrane protein with an extracellular ligand- binding domain of 512 amino acids, an intramembrane domain of 25 amino acids, and a cytoplasmic domain of 435 amino acids encoding a tyrosine kinase.Human M-CSF is active in mouse and rat cells. The murine factor is active in rat cells but inactive in human cells.M- CSF is a specific factor in that the proliferation inducing activity is more or less restricted to the macrophage lineage. M-CSF is a potent stimulator of functional activities of monocytes.

g M-CSF and il-4 induce dendritic cell differentiation and disrupt osteoclastogenesis through M-CSF receptor shedding by up-regulation of tnf- converting enzyme (tace) . Blood, Sep 2009; 10.1182/blood-2009-04-215020. g M-CSF - and M-CSF -dependent macrophage phenotypes display differential dependence on type i interferon signaling . J. Leukoc. Biol., Aug 2009; 86: 411 - 421. M-CSF inhibition selectively targets pathological angiogenesis and lymphangiogenesis . J. Exp. Med., May 2009; 206: 1089 - 1102. c-reactive protein induces M-CSF release and macrophage proliferation . J. Leukoc. Biol., Feb 2009; 85: 262 - 267. M-CSF preferentially induces c-fos via erk to specify monopoiesis whereas g-csf directs granulopoiesis via shp2 . Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 2887.

27262661

NP_000748.3

NM_000757.5

P09603

60,179 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Integrins In Angiogenesis Pathway (137999); Osteoclast Differentiation Pathway (193147); Osteoclast Differentiation Pathway (193096); Rheumatoid Arthritis Pathway (200309); Rheumatoid Arthritis Pathway (200269)

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]

M-CSF: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers. Aberrant expression of CSF1 or CSF1R may play a role in inflammatory diseases, such as rheumatoid arthritis, glomerulonephritis, atherosclerosis, and allograft rejection. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cytokine; Membrane protein, integral. Chromosomal Location of Human Ortholog: 1p13.3. Cellular Component: extracellular space; membrane; perinuclear region of cytoplasm; plasma membrane; integral to membrane; receptor complex. Molecular Function: protein homodimerization activity; growth factor activity; cytokine activity; macrophage colony stimulating factor receptor binding. Biological Process: positive regulation of monocyte differentiation; ossification; macrophage differentiation; positive regulation of osteoclast differentiation; positive regulation of cellular protein metabolic process; positive regulation of odontogenesis of dentine-containing teeth; reproductive developmental process; osteoclast differentiation; positive regulation of multicellular organism growth; odontogenesis; cell proliferation; monocyte activation; positive regulation of mononuclear cell proliferation; homeostasis of number of cells within a tissue; positive regulation of protein kinase activity; positive regulation of cell proliferation; innate immune response; hemopoiesis; positive regulation of Ras protein signal transduction; positive regulation of cell-matrix adhesion; inflammatory response; positive regulation of macrophage differentiation; cell differentiation; regulation of ossification; positive regulation of cell migration

0.1 mg

280 USD

0.2 mg

385