Rabbit anti CD3 Polyclonal | MyBioSource MBS551029 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti CD3 Polyclonal

catalog :

MBS551029

quantity :

0.1 mg

price :

280 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of FFPE rat kidney sections showing lymphocyte expression of CD3. It was used as primary antibody, dilution 1:200, DAB chromogenic staining.

image 2 :

Immunoflourescence of human colon cancer sections showing expression of CD3. MBS551029 was used as a primary antibody , dilution 1:60.

product information

catalog number :

MBS551029

products type :

Antibody

products full name :

Rabbit anti CD3 Polyclonal

products short name :

[CD3]

products name syn :

[Rabbit anti Human CD3 Polyclonal purified; cd3; cd-3; polyclonal; PAb]

other names :

[T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain]

products gene name :

[CD3]

other gene names :

[CD3E; T3E]

uniprot entry name :

CD3E_HUMAN

clonality :

polyclonal

isotype :

IgG

host :

rabbit

reactivity :

Human, Mouse, Rat

sequence length :

207

purity :

Purity: >95%. Purification: Serum IgG fraction was purified by Protein G affinity chromatography.

form :

Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.

storage stability :

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. Shelf Life: >12 months

tested application :

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

app notes :

Western Blot: A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IF: A suitable range of concentrations of this antibody for IHC is 10-20 ug/ml. Immunoflourescence analysis of CD3-expressison in Human colon cancer tissue. It was used as primary antibody, dilution 1:60.

image1 heading :

Immunohistochemistry

image2 heading :

Test Data

other info1 :

Source Note: Polyclonal CD3 antibody was produced from sera of rabbits immunized with synthetic protein containing CD3 antigenic determinants.

other info2 :

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers. Presentation: Lyophilzed from 0.2um filtered solution in PBS pH 7.4. Dilution: A quick spin of the vial followed by reconstitution 0.01M PBS pH 7.4 or other diluents.

products categories :

Polyclonal

products description :

The membrane glycoprotein CD34 has been proven to be a useful marker for human hematopoietic stem/progenitor cells, and various types of colony- forming activity in human bone marrow, cord blood, and mobilized peripheral blood are contained in the CD34+ population.

products references :

integrin and CD3 /tcr activation are regulated by the scaffold protein akap450 . Blood, Mar 2010; 10.1182/blood-2009-12-256222. complex interactions between the major and minor envelope proteins of equine arteritis virus determine its tropism for equine CD3 + t lymphocytes and cd14+ monocytes . J. Virol., Mar 2010; 10.1128/JVI.02743-09. il-7 and il-15 independently program the differentiation of intestinal CD3 nkp46+ cell subsets from id2-dependent precursors . J. Exp. Med., Feb 2010; 207: 273 - 280. transient epstein-barr virus reactivation in CD3 monoclonal antibody-treated patients . Blood, Feb 2010; 115: 1145 - 1155. pharmacokinetics and antibody responses to the CD3 antibody otelixizumab used in the treatment of type 1 diabetes . J. Clin. Pharmacol., Feb 2010; 10.1177/0091270009356299.

ncbi gi num :

4502671

ncbi acc num :

NP_000724.1

ncbi gb acc num :

NM_000733.3

uniprot acc num :

P07766

ncbi mol weight :

23,147 Da

ncbi pathways :

Adaptive Immunity Signaling Pathway (366160); CXCR4-mediated Signaling Events Pathway (137910); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Costimulation By The CD28 Family Pathway (119552); Downstream TCR Signaling Pathway (106418); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); Generation Of Second Messenger Molecules Pathway (106417); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889)

ncbi summary :

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

uniprot summary :

CD3E: a T cell surface glycoprotein that is a component of the T cell antigen receptor. The recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Contains 1 immunoglobulin-like domain and 1 ITAM domain. Protein type: Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 11q23. Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; immunological synapse; alpha-beta T cell receptor complex; intercellular junction; external side of plasma membrane. Molecular Function: protein binding; transmembrane receptor activity; receptor signaling complex scaffold activity; protein heterodimerization activity; receptor signaling protein activity; T cell receptor binding; protein kinase binding; SH3 domain binding. Biological Process: regulation of immune response; T cell activation; positive regulation of interleukin-2 biosynthetic process; signal complex assembly; positive regulation of calcium-mediated signaling; T cell receptor signaling pathway; negative thymic T cell selection; positive regulation of interleukin-4 production; regulation of apoptosis; G-protein coupled receptor protein signaling pathway; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of interferon-gamma production; cell surface receptor linked signal transduction; negative regulation of smoothened signaling pathway; T cell costimulation; positive regulation of T cell proliferation; protein complex assembly; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell anergy; response to nutrient; transmembrane receptor protein tyrosine kinase signaling pathway. Disease: Immunodeficiency 18

size1 :

0.1 mg

price1 :

280 USD

size2 :

0.2 mg

price2 :

385

more info or order :

MyBioSource product webpage