antibody

Rabbit anti IGF-I Polyclonal

MBS551027

0.1 mg

280 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Rabbit anti IGF-I Polyclonal

IGF-I

Rabbit anti Human IGF-I Polyclonal purified; IGF1; IGF-1; igf-i; pab; polyclonal

insulin-like growth factor 1 isoform 4 preproprotein; Insulin-like growth factor I; Mechano growth factor; MGF; Somatomedin-C

IGF-I

IGF1; IBP1; IGF-I; MGF

IGF1_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

153

Serum IgG fraction was purified by Protein G affinity chromatography. Purity: >95%

Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. Shelf Life: >12 months

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of IGF-I expression in NZ rabbit knee joint tissue. It was used as primary antibody, dilution 1:200.

Source Note: Polyclonal IGF-I antibody was produced from sera of rabbits immunized with synthetic protein containing IGF-1 C-terminal antigenic determinants.

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers. Presentation: Lyophilized from 0.2um filtered solution in PBS pH 7.4. Dilution: A quick spin of the vial followed by reconstitution in 0.01M PBS pH 7.4 or other diluents. Source Information: Polyclonal anti IGF-I was produced from sera of rabbits immunized with synthetic protein containing IGF-I C-terminal antigenic determinants.

Polyclonal

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I is synthesized as two precursor isoforms with N- and alternate C- terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF- I is identical between isoforms and is generated by proteolytic removal of the N- and C- terminal regions. Mature human IGF-I shares 94% and 96% aa sequence identity with mouse and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF-II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects.

mechano-transduction in osteoblastic cells involves strain-regulated estrogen receptor -mediated control of insulin-like growth factor (igf) i receptor sensitivity to ambient igf, leading to phosphatidylinositol 3-kinase/akt-dependent wnt/lrp5 receptor-independent activation of -catenin signaling . J. Biol. Chem., Mar 2010; 285: 8743 - 8758. IGF-I lucidity. the murky waters begin to clear? . J Appl Physiol, Mar 2010; 10.1152/japplphysiol.00252.2010. a significant decline in IGF-I may predispose young africans to subsequent cardiometabolic vulnerability . J. Clin. Endocrinol. Metab., Mar 2010; 10.1210/jc.2009-2329. insulin-like growth factor-1 ( IGF-I ) stimulated insulin receptor substrate-1 negatively regulates src homology 2 domain-containing protein-tyrosine phosphatase substrate-1 function in vascular smooth muscle cells . J. Biol. Chem., Mar 2010; 10.1074/jbc.M109.092270. deletion of the IGF-I gene: suppressive effects on adult leydig cell development . J Androl, Mar 2010; 10.2164/jandrol.109.008680.

11024682

NP_000609.1

NM_000618.3

P01343

21,841 Da

Adipogenesis Pathway (198832); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Androgen Receptor Signaling Pathway (198806); Apoptosis Pathway (198797); Diabetes Pathways (105902); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Endochondral Ossification Pathway (198812); Focal Adhesion Pathway (198795)

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]

IGF1: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency). IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. Belongs to the insulin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 12q23.2. Cellular Component: insulin-like growth factor binding protein complex; extracellular space; plasma membrane; extracellular region. Molecular Function: integrin binding; insulin-like growth factor receptor binding; protein binding; growth factor activity; hormone activity; insulin receptor binding. Biological Process: muscle development; positive regulation of transcription, DNA-dependent; chondroitin sulfate proteoglycan biosynthetic process; glycolate metabolic process; exocrine pancreas development; water homeostasis; positive regulation of glucose import; positive regulation of fibroblast proliferation; proteoglycan biosynthetic process; inner ear development; positive regulation of DNA binding; muscle hypertrophy; platelet activation; positive regulation of mitosis; positive regulation of protein import into nucleus, translocation; regulation of establishment and/or maintenance of cell polarity; positive regulation of phosphoinositide 3-kinase cascade; cell activation; positive regulation of peptidyl-tyrosine phosphorylation; branching morphogenesis of a tube; insulin-like growth factor receptor signaling pathway; response to heat; regulation of gene expression; positive regulation of transcription from RNA polymerase II promoter; alveolus development; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of smooth muscle cell proliferation; myoblast proliferation; positive regulation of glycogen biosynthetic process; positive regulation of smooth muscle cell migration; signal transduction; positive regulation of activated T cell proliferation; negative regulation of cell proliferation; platelet degranulation; glial cell differentiation; positive regulation of MAPKKK cascade; mammary gland development; positive regulation of cell proliferation; DNA replication; skeletal development; positive regulation of granule cell precursor proliferation; phosphoinositide-mediated signaling; multicellular organism growth; myotube cell development; regulation of multicellular organism growth; satellite cell compartment self-renewal involved in skeletal muscle regeneration; myoblast differentiation; positive regulation of protein kinase B signaling cascade; positive regulation of osteoblast differentiation; cell proliferation; cellular protein metabolic process; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of glycolysis; Ras protein signal transduction; blood vessel remodeling; positive regulation of Ras protein signal transduction; blood coagulation; cell motility; positive regulation of DNA replication. Disease: Insulin-like Growth Factor I Deficiency

0.1 mg

280 USD

0.2 mg

385