Rabbit anti Insulin Polyclonal | MyBioSource MBS551026 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti Insulin Polyclonal

catalog :

MBS551026

quantity :

0.1 mg

price :

280 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Imunohistochemical analysis of Insulin expression in formalin fixed mouse pancreatic mouse. MBS551026 was used as primary antibody, dilution 1:200,400x

product information

catalog number :

MBS551026

products type :

Antibody

products full name :

Rabbit anti Insulin Polyclonal

products short name :

[Insulin]

products name syn :

[Rabbit anti Human Insulin Polyclonal purified; insulin; pab; polyclonal]

other names :

[insulin preproprotein; Insulin]

products gene name :

[Insulin]

other gene names :

[INS]

uniprot entry name :

INS_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

110

purity :

>95%. Serum IgG fraction was purified by Protein G affinity chromatography.

form :

Lyophilized from 0.2 um filtered solution in PBS pH 7.4

storage stability :

The lyophilized antibody is stable for at least 1 year from date of receipt at -20°C. Upon reconstitution, this antibody can be stored in working aliquots at 2-8°C for one month, or at -20°C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles.

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

ELISA: Use at a concentration range of 1-2 ug/ml. Western Blot: Use at a concentration range of 2-10 ug/ml. Immunohistochemistry: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.

image1 heading :

Immunohistochemistry

other info1 :

Host Note: Polyclonal anti Insulin was produced from sera of rabbits immunized with synthetic protein containing Insulin C-terminal antigenic determinants.

other info2 :

Dilution: A quick spin of the vial followed by reconstitution in 0.01M PBS, pH 7.4 or other diluents.

products categories :

Polyclonal

products references :

fxr activation reverses Insulin resistance and lipid abnormalities and protects against liver steatosis in zucker (fa/fa) obese rats . J. Lipid Res., Apr 2010; 51: 771 - 784. expression of tau in Insulin -secreting cells and its interaction with the calcium-binding protein secretagogin . J. Endocrinol., Apr 2010; 205: 25 - 36. silencing of three amblyomma americanum (l.) Insulin -like growth factor binding protein-related proteins prevents ticks from feeding to repletion . J. Exp. Biol., Apr 2010; 213: 1153 - 1161. sclerocarya birrea (anacardiaceae) stem-bark extract corrects glycaemia in diabetic rats and acts on -cells by enhancing glucose-stimulated Insulin secretion . J. Endocrinol., Apr 2010; 205: 79 - 86. atorvastatin causes Insulin resistance and increases ambient glycemia in hypercholesterolemic patients . J. Am. Coll. Cardiol., Mar 2010; 55: 1209 - 1216.

ncbi gi num :

4557671

ncbi acc num :

NP_000198.1

ncbi gb acc num :

NM_000207.2

uniprot acc num :

P01308

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); Adipogenesis Pathway (198832); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Amyloids Pathway (366238); Arf6 Trafficking Events Pathway (137954); Diabetes Pathways (105902); Downstream Signaling Of Activated FGFR Pathway (160957); FOXA1 Transcription Factor Network Pathway (137979); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911)

ncbi summary :

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

uniprot summary :

Insulin: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Heterodimer of a B chain and an A chain linked by two disulfide bonds. Belongs to the insulin family. Protein type: Secreted, signal peptide; Secreted; Hormone. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: extracellular space; Golgi lumen; endoplasmic reticulum lumen; extracellular region. Molecular Function: identical protein binding; insulin-like growth factor receptor binding; protein binding; protease binding; hormone activity; insulin receptor binding. Biological Process: positive regulation of nitric oxide biosynthetic process; glucose homeostasis; positive regulation of vasodilation; activation of NF-kappaB transcription factor; positive regulation of glucose import; negative regulation of protein oligomerization; regulation of protein localization; cell-cell signaling; negative regulation of gluconeogenesis; acute-phase response; regulation of transmembrane transporter activity; negative regulation of NAD(P)H oxidase activity; positive regulation of mitosis; positive regulation of nitric-oxide synthase activity; activation of protein kinase B; negative regulation of acute inflammatory response; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of protein amino acid autophosphorylation; positive regulation of cell growth; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of cell differentiation; regulation of amino acid metabolic process; negative regulation of protein catabolic process; negative regulation of glycogen catabolic process; wound healing; positive regulation of glycogen biosynthetic process; positive regulation of cellular protein metabolic process; glucose transport; regulation of protein secretion; negative regulation of vasodilation; regulation of transcription, DNA-dependent; negative regulation of lipid catabolic process; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; negative regulation of proteolysis; negative regulation of protein secretion; MAPKKK cascade; negative regulation of fatty acid metabolic process; alpha-beta T cell activation; endocrine pancreas development; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; cellular protein metabolic process; fatty acid homeostasis; positive regulation of glycolysis; insulin receptor signaling pathway; energy reserve metabolic process; positive regulation of insulin receptor signaling pathway; regulation of insulin secretion; positive regulation of DNA replication; positive regulation of cell migration; positive regulation of cytokine secretion. Disease: Diabetes Mellitus, Insulin-dependent, 2; Diabetes Mellitus, Permanent Neonatal; Hyperproinsulinemia; Maturity-onset Diabetes Of The Young, Type 10

size1 :

0.1 mg

price1 :

280 USD

size2 :

0.2 mg

price2 :

385

more info or order :

MyBioSource product webpage