Rabbit anti IL-8 Polyclonal | MyBioSource MBS551025 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti IL-8 Polyclonal

catalog :

MBS551025

quantity :

0.1 mg

price :

280 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS551025

products type :

Antibody

products full name :

Rabbit anti IL-8 Polyclonal

products short name :

IL-8

products name syn :

Rabbit anti IL-8 Polyclonal purified; il-8; il8; NAP-1; nap1; cxcl8; pab; polyclonal

other names :

interleukin-8; Interleukin-8; C-X-C motif chemokine 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1; Monocyte-derived neutrophil chemotactic factor; MDNCF; Monocyte-derived neutrophil-activating peptide; MONAP; Neutrophil-activating protein 1; NAP-1; Protein 3-10C; T-cell chemotactic factorCleaved into the following 7 chains:MDNCF-a; Alternative name(s):; GCP/IL-8 protein IV; IL8/NAP1 form IInterleukin-8; Alternative name(s):; (Ala-IL-8)77; GCP/IL-8 protein II; IL-8(1-77); IL8/NAP1 form II; MDNCF-bIL-8(5-77)IL-8(6-77); Alternative name(s):; (Ser-IL-8)72; GCP/IL-8 protein I; IL8/NAP1 form III; Lymphocyte-derived neutrophil-activating factor; LYNAP; MDNCF-c; Neutrophil-activating factor; NAFIL-8(7-77); Alternative name(s):; GCP/IL-8 protein V; IL8/NAP1 form IVIL-8(8-77); Alternative name(s):; GCP/IL-8 protein VI; IL8/NAP1 form VIL-8(9-77); Alternative name(s):; GCP/IL-8 protein III; IL8/NAP1 form VI

products gene name :

IL-8

other gene names :

IL8; CXCL8; IL-8; GCP-1; MDNCF; MONAP; NAP-1; LYNAP; NAF

uniprot entry name :

IL8_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

purity :

Serum IgG fraction was purified by Protein G affinity chromatography. >95%

form :

Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.

storage stability :

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. Shelf Life: >12 months

tested application :

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

app notes :

ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml.

other info1 :

Source Note: Polyclonal Interleukin-8 antibody was produced from sera of rabbits immunized with synthetic protein containing Interleukin-8 antigenic determinants. Presentation: Lyophilized from 0.2um filtered solution in PBS pH 7.4. Dilution: A quick spin of the vial followed by reconstitution in 0.01M PBS pH 7.4 or other diluents.

products categories :

Polyclonal

products description :

IL8 is produced by stimulated monocytes but not by tissue macrophages and T- lymphocytes. In many cell types the synthesis of IL8 is strongly stimulated by IL1 and TNF- alpha. In human skin fibroblasts the expression of IL-8 is enhanced by Leukoregulin.The expression of IL-8 from resting and stimulated human blood monocytes is up-regulated by IL-7. In chondrocytes the synthesis of IL8 is stimulated by IL1-beta TNF- alpha and bacterial lipopolysaccharides. In human astrocytes the synthesis and secretion of IL8 is induced by IL-1 and TNF-alpha. The IL8 receptor is a dimeric glycoprotein consisting of a 59 kDa and a 67 kDa subunit. It has been given the name CD128. It is expressed in many different cell types including those not responding to IL8. The biological activities of IL8 resemble those of a related protein, NAP-2. IL8 differs from all other cytokines in its ability to specifically activate neutrophil granulocytes. In neutrophils IL8 causes a transient increase in cytosolic calcium levels and the release of enzymes from granules. IL-8 also enhances the metabolism of reactive oxygen species and increases Chemotaxis and the enhanced expression of adhesion molecules. A pre-activation by IL3 is required to render basophils and neutrophils susceptible to further activation by IL8.

products references :

cell surface-bound il-1 is an upstream regulator of the senescence-associated il-6/ IL-8 cytokine network . PNAS, Sep 2009; 10.1073/pnas.0905299106. the angiogenic effect of probiotic bacillus polyfermenticus on human intestinal microvascular endothelial cells is mediated by IL-8 . Am J Physiol Gastrointest Liver Physiol, Sep 2009; 10.1152/ajpgi.00204.2009. IL-8 signaling does not mediate intra-amniotic lps-induced inflammation and maturation in preterm fetal lamb lung . Am J Physiol Lung Cell Mol Physiol, Sep 2009; 297: L512 - L519. the functional significance behind expressing two IL-8 receptor types on pmn . J. Leukoc. Biol., Sep 2009; 86: 529 - 543. IL-8 regulates mucin gene expression at the posttranscriptional level in lung epithelial cells . J. Immunol., Aug 2009; 183: 2159 - 2166.

ncbi gi num :

10834978

ncbi acc num :

NP_000575.1

ncbi gb acc num :

NM_000584.3

uniprot acc num :

P10145

ncbi mol weight :

11,098 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051)

ncbi summary :

The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq, Jul 2008]

uniprot summary :

IL8: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. Belongs to the intercrine alpha (chemokine CxC) family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cytokine; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 4q13-q21. Cellular Component: extracellular space; extracellular region. Molecular Function: protein binding; chemokine activity; interleukin-8 receptor binding. Biological Process: regulation of cell adhesion; neutrophil chemotaxis; neutrophil activation; unfolded protein response; negative regulation of G-protein coupled receptor protein signaling pathway; calcium-mediated signaling; chemotaxis; signal transduction; regulation of retroviral genome replication; induction of positive chemotaxis; G-protein coupled receptor protein signaling pathway; negative regulation of cell proliferation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; receptor internalization; response to molecule of bacterial origin; immune response; angiogenesis; cell cycle arrest; cell motility; inflammatory response; embryonic gut development

size1 :

0.1 mg

price1 :

280 USD

size2 :

0.2 mg

price2 :

385

more info or order :

MyBioSource product webpage