Rabbit anti CD20 Polyclonal | MyBioSource MBS551023 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti CD20 Polyclonal

catalog :

MBS551023

quantity :

0.1 mg

price :

280 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS551023

products type :

Antibody

products full name :

Rabbit anti CD20 Polyclonal

products short name :

CD20

products name syn :

Rabbit anti CD20 Polyclonal purified; cd20; cd-20; polyclonal

other names :

B-lymphocyte antigen CD20; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1

products gene name :

CD20

other gene names :

MS4A1; CD20

uniprot entry name :

CD20_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

297

purity :

Purity: >95%. Purification: Serum IgG fraction was purified by Protein G affinity chromatography.

form :

Lyophilized from 0.2um filtered solution in PBS pH 7.4

storage stability :

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. Shelf Life: >12 months

tested application :

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

app notes :

ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of FFPE Human colon cancer sections expressing CD20. Immunohistochemical analysis of FFPE mouse groin lymphoma sections expressing CD20.

other info1 :

Source Note: Polyclonal CD20 antibody was produced from sera of rabbits immunized with synthetic protein containing CD20 antigenic determinants.

other info2 :

Reconstitution: A quick spin of the vial followed by reconstitution in 0.01M PBS pH 7.4 or other diluents.

products categories :

Polyclonal

products description :

CD20 is a signature B-cell differentiation antigen strongly expressed on the surface of mature normal as well as malignant B cells. It is also expressed, but at lower levels and with larger variance, on more immature B cells and their malignant counterparts found in B-cell precursor (BCP) acute lymphoblastic leukemia. The precise function of CD20 remains unknown, it appears to act as part of an ion channel expressed in the plasma membrane,recently shown that CD20 may operate as a store operated calcium (Ca2+) channel facilitating entry of extracellular Ca2+ following BCR-induced emptying of intracellular stores. Interestingly, despite this presumably important role, CD20- deficient mice are without obvious phenotype, which suggests that either this function is not critical for B cell physiology or that molecular redundancy exists.

products references :

antigenic modulation limits the efficacy of anti- CD20 antibodies: implications for antibody selection . Blood, Jun 2010; 115: 5191 - 5201. increasing the efficacy of CD20 antibody therapy through the engineering of a new type ii anti- CD20 antibody with enhanced direct and immune effector cell mediated b-cell cytotoxicity . Blood, Jun 2010; 115: 4393 - 4402. antifungal therapy with itraconazole impairs the anti-lymphoma effects of rituximab by inhibiting recruitment of CD20 to cell surface lipid rafts . Cancer Res., Jun 2010; 70: 4292 - 4296. expanded cd23+/cd21hi b cells in inflamed lymph nodes are associated with the onset of inflammatory-erosive arthritis in tnf-transgenic mice and are targets of anti- CD20 therapy . J. Immunol., Jun 2010; 184: 6142 - 6150. CD20 -immunotargeting of malignant melanoma. . ASCO Meeting Abstracts, May 2010; 28: 8565.

ncbi gi num :

23110987

ncbi acc num :

NP_068769.2

ncbi gb acc num :

NM_021950.3

uniprot acc num :

P11836

ncbi mol weight :

33,077 Da

ncbi pathways :

Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489)

ncbi summary :

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]

uniprot summary :

MS4A1: This protein may be involved in the regulation of B-cell activation and proliferation. Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the MS4A family. Protein type: Membrane protein, integral; Cell surface; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 11q12. Cellular Component: extracellular space; integral to plasma membrane; external side of plasma membrane. Molecular Function: epidermal growth factor receptor binding. Biological Process: B cell proliferation; humoral immune response. Disease: Immunodeficiency, Common Variable, 5

size1 :

0.1 mg

price1 :

280 USD

size2 :

0.2 mg

price2 :

385

more info or order :

MyBioSource product webpage