Rabbit Anti Human IL-4 Polyclonal | MyBioSource MBS551020 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti Human IL-4 Polyclonal

catalog :

MBS551020

quantity :

0.1 mg

price :

280 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

product information

catalog number :

MBS551020

products type :

Antibody

products full name :

Rabbit Anti Human IL-4 Polyclonal

products short name :

IL-4

products name syn :

Rabbit Anti IL-4 Polyclonal purified; il-4; il4; interleukin-4; bsf-1; polyclonal; pab

other names :

interleukin-4 isoform 1; Interleukin-4; B-cell stimulatory factor 1; BSF-1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra

products gene name :

IL-4

other gene names :

IL4; IL-4; BSF-1

uniprot entry name :

IL4_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

153

purity :

Serum IgG fraction was purified by Protein G affinity chromatography.

form :

Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.

storage stability :

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. Shelf Life: >12 months

tested application :

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

app notes :

ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of IL-4 expression in formalin fixed paraffin embedded sections of Human esophageal squamous cell carcinoma. It was used as primary antibody, dilution 1:200.

other info1 :

Source Note: Polyclonal Interleukin-4 antibody was produced from sera of rabbits immunized with synthetic protein containing N- terminal Interleukin-4 antigenic determinants.

other info2 :

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers

products categories :

Polyclonal

products description :

Interleukin-4 is produced mainly by a subpopulation of activated T-cells (Th2) which are the biologically most active helper cells for B-cells and which also secrete IL-5 and IL6. The biological activities of IL-4 are mediated by a specific receptor The extracellular domain of the IL4 receptor is related to the receptors for EPO, IL6, and the beta chain of the IL2 receptor. It has been given the name CD124. Two types of IL4 receptor (IL4R) exist: the type 1 receptor is a heterodimer consisting of CD132 and IL4R-alpha. The type 2 receptor is a heterodimer consisting of IL4R-alpha and IL13R-alpha- 1. IL4 enhances expression of MHC class 2 antigens on B- cells. It can promote their capacity to respond to other B-cell stimuli and to present antigens for T- cells.Pretreatment of macrophages with IL4 prevents the production of IL1, TNF-alpha and prostaglandins in response to activation of the cells by bacterial endotoxins or IFN-gamma.

products references :

pathogenicity of a disease-associated human IL-4 receptor allele in experimental asthma . J. Exp. Med., Sep 2009; 206: 2191 - 2204. complex regulation of tartrate-resistant acid phosphatase (trap) expression by interleukin 4 ( IL-4 ) : IL-4 indirectly suppresses receptor activator of nf -kappab ligand (rankl) - mediated trap expression but modestly induces its expression directly . J. Biol. Chem., Sep 2009; 10.1074/jbc.M109.001016. gm-csf and IL-4 induce dendritic cell differentiation and disrupt osteoclastogenesis through m-csf receptor shedding by up-regulation of tnf- converting enzyme (tace) . Blood, Sep 2009; 10.1182/blood-2009-04-215020. ship represses the generation of il-3-induced m2 macrophages by inhibiting IL-4 production from basophils . J. Immunol., Sep 2009; 183: 3652 - 3660. mycobacterium tuberculosis culture filtrate proteins plus cpg-odn confer protection to bcg primed mice by inhibiting IL-4 secretion . Infect. Immun., Sep 2009; 10.1128/IAI.00580-09.

ncbi gi num :

4504669

ncbi acc num :

NP_000580.1

ncbi gb acc num :

NM_000589.3

uniprot acc num :

P05112

ncbi mol weight :

17,492 Da

ncbi pathways :

Allograft Rejection Pathway 83123!!Allograft Rejection Pathway 535!!Asthma Pathway 83120!!Asthma Pathway 532!!Autoimmune Thyroid Disease Pathway 83121!!Autoimmune Thyroid Disease Pathway 533!!CD40/CD40L Signaling Pathway 138061!!Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway 137993!!Calcium Signaling In The CD4+ TCR Pathway 137941!!Cytokine-cytokine Receptor Interaction Pathway 83051

uniprot summary :

Function: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Subcellular location: Secreted. Involvement in disease: Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.19. Sequence similarities: Belongs to the IL-4/IL-13 family.

size :

0.1 mg

price :

280 USD