Rabbit anti IL-1 alpha polyclonal | MyBioSource MBS551016 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti IL-1 alpha polyclonal

catalog :

MBS551016

quantity :

0.1 mg

price :

280 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS551016

products type :

Antibody

products full name :

Rabbit anti IL-1 alpha polyclonal

products short name :

IL-1 alpha

products name syn :

Rabbit anti IL-1 alpha polyclonal purified; il-1 alpha; il1a; il1; interleukin-1; il-1a; polyclonal; pab

other names :

interleukin-1 alpha proprotein; Interleukin-1 alpha; Hematopoietin-1

products gene name :

IL-1

other gene names :

IL1A; IL1F1; IL-1 alpha

uniprot entry name :

IL1A_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mouse, Rat

sequence length :

271

purity :

> 95%

form :

Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.

storage stability :

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. Shelf Life: >12 months

tested application :

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

app notes :

ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.

other info1 :

Source Note: Polyclonal IL-1 alpha antibody was produced from sera of rabbits immunized with synthetic protein containing Interleukin-1 alpha antigenic determinants.

other info2 :

Presentation: 0.2um filtered 1 mg/ml solution in TBS pH 7.4, 50% glycerol, 1% BSA, 0.03% proclin. Dilution: A quick spin of the vial follow by reconsitution in 0.01M PBS pH 7.4 or other diluents.

products categories :

Polyclonal

products description :

Monocytes are the main source of secreted IL1. They express predominantly IL1- beta while human keratinocytes express large amounts of IL1-alpha. Murine macrophages display a transition from IL1-beta to IL1-alpha production during maturation of monocytes into inflammatory macrophages.There are two functionally almost equivalent forms of IL1, IL1-alpha and IL1-beta that are encoded by two different genes. IL1-beta is the predominant form in humans while it is IL1-alpha in mice. Both forms of IL1 bind to the same receptor and therefore also show similar if not identical biological activities. The IL1-beta but not the IL1-alpha precursor must be processed before it can bind to the receptor. Both forms of IL1 bind to the same receptor and therefore also show similar if not identical biological activities. The receptor isolated from T-cells is expressed predominantly on T-cells and cells of mesenchymal origin. It binds both types of IL1 with equal affinity. This type is called also Type 1 receptor. It has been designated CD121a. The Type 2 receptor has been designated CD121b. It is isolated from B-cells, granulocytes, and macrophages. It is expressed predominantly on B-cells and cells of the myelomonocytic lineage and is encoded by a separate gene.

products references :

cell surface-bound il-1 is an upstream regulator of the senescence-associated il-6/il-8 cytokine network . PNAS, Sep 2009; 10.1073/pnas.0905299106. expression of bfgf and IL-1 alpha in tears of galactosemic rats: implications for wound healing and neovascularization . Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 545. cutting edge: critical role for mesothelial cells in necrosis-induced inflammation through the recognition of il-1 released from dying cells . J. Immunol., Dec 2008; 181: 8194 - 8198. influence of interleukin 1 (il-1), il-4, and il-6 polymorphisms on genetic susceptibility to chronic osteomyelitis . Clin. Vaccine Immunol., Dec 2008; 15: 1888 - 1890. role of acute and prolonged IL-1 alpha & beta induction on primary culture of primary porcine culture . Invest. Ophthalmol. Vis. Sci., Apr 2008; 49: 1657.

ncbi gi num :

27894330

ncbi acc num :

NP_000566.3

ncbi gb acc num :

NM_000575.3

uniprot acc num :

P01583

ncbi mol weight :

30,607 Da

ncbi pathways :

Apoptosis Pathway (83060); Apoptosis Pathway (470); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); FAS Pathway And Stress Induction Of HSP Regulation (198894); Graft-versus-host Disease Pathway (83124); Graft-versus-host Disease Pathway (536); Hematopoietic Cell Lineage Pathway (83078)

ncbi summary :

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]

uniprot summary :

IL1A: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Belongs to the IL-1 family. Protein type: Cytokine; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 2q14. Cellular Component: extracellular space; extracellular region; cytosol. Molecular Function: protein binding; copper ion binding; interleukin-1 receptor binding; cytokine activity. Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of mitosis; apoptosis; cytokine and chemokine mediated signaling pathway; positive regulation of interleukin-2 biosynthetic process; germ cell programmed cell death; positive regulation of JNK cascade; fever; negative regulation of cell proliferation; cell proliferation; connective tissue replacement during inflammatory response; positive regulation of angiogenesis; response to copper ion; positive regulation of transcription from RNA polymerase II promoter; immune response; inflammatory response; positive regulation of cytokine secretion

size1 :

0.1 mg

price1 :

280 USD

size2 :

0.2 mg

price2 :

385

more info or order :

MyBioSource product webpage