antibody

Rabbit anti BMP-4 Polyclonal

MBS551014

0.1 mg

280 USD

polyclonal

rabbit

BMP

human, mouse, rat

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Rabbit anti BMP-4 Polyclonal

BMP-4

Rabbit anti BMP-4 Polyclonal purified; Bone Morphogenetic protein ; bmp-4; bmp4; polyclonal; PAb

bone morphogenetic protein 4 preproprotein; Bone morphogenetic protein 4; Bone morphogenetic protein 2B

BMP-4

BMP4; BMP2B; DVR4; BMP-4; BMP-2B

BMP4_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

408

Serum IgG fraction was purified by Protein G affinity chromatography.

Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. Shelf Life: >12 months

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.

Source Note: Polyclonal BMP-4 antibody was produced from sera of rabbits immunized with synthetic protein containing BMP-4 antigenic determinants.

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers

Polyclonal

BMPs are proteins that act to induce the differentiation of mesenchymal-type cells into chondrocytes and osteoblasts before initiating bone formation. They promote the differentiation of cartilage-forming cells and bone-forming cells near sites of fractures but also at ectopic locations. Some of the proteins induce the synthesis of alkaline phosphatase and collagen in osteoblasts. Some BMPs act directly on osteoblasts and promote their maturation while at the same time suppressing myogenous differentiation. Other BMPs promote the conversion of typical fibroblasts into chondrocytes and are capable also of inducing the expression of an osteoblast phenotype in non-osteogenic cell types. Intracellular signaling following engagement of receptors for some BMP proteins has been shown to involve the action of SMAD proteins. BMP4 and BMP7 have been shown to be involved also in the differentiation of sympathetic neurons. They enhance the formation of adrenergic sympathetic neurons in cultures of neural crest cells.

smad, pi3k/akt, and wnt-dependent signaling pathways are involved in BMP-4 -induced esc self-renewal . Stem Cells, Aug 2009; 27: 1858 - 1868. molecular interaction of BMP-4 , tgf- , and estrogens in lactotrophs: impact on the prl promoter . Mol. Endocrinol., Jul 2009; 23: 1102 - 1114. suppression of vegf expression and lesion size in laser-induced cnv in a mouse model overexpressing BMP-4 . Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 1193. altered shear stress stimulates upregulation of endothelial vcam-1 and icam-1 in a BMP-4 and tgf- 1 dependent pathway . Arterioscler Thromb Vasc Biol, Feb 2009; 29: 254 - 260. the bone morphogenetic protein (bmp) -4 is involved in the regulation of human megakaryocytic differentiation during thrombopoietin signaling. . Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 1339.

157276593

NP_001193.2

NM_001202.3

P12644

46,555 Da

Adipogenesis Pathway 198832!!Basal Cell Carcinoma Pathway 83113!!Basal Cell Carcinoma Pathway 525!!Glypican 3 Network Pathway 138084!!Heart Development Pathway 198802!!Hedgehog Signaling Pathway 83063!!Hedgehog Signaling Pathway 474!!Pathways In Cancer 83105!!TGF Beta Signaling Pathway 198810!!TGF-beta Signaling Pathway 83064

Function: Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction . By similarity. Subunit structure: Homodimer; disulfide-linked . By similarity. Interacts with GREM2. Part of a complex consisting of TWSG1 and CHRD. Interacts with the serine proteases, HTRA1 and HTRA3; the interaction with either inhibits BMP4-mediated signaling. The HTRA protease activity is required for this inhibition . By similarity. Interacts with SOSTDC1. Ref.5. Subcellular location: Secreted extracellular space extracellular matrix. Tissue specificity: Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines. Involvement in disease: Microphthalmia, syndromic, 6 (MCOPS6) [MIM:607932]: A disease characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies. Microphthalmia is a disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.7Non-syndromic orofacial cleft 11 (OFC11) [MIM:600625]: A birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.9. Sequence similarities: Belongs to the TGF-beta family.

0.1 mg

280 USD