Rabbit anti IGF-II Polyclonal | MyBioSource MBS551011 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti IGF-II Polyclonal

catalog :

MBS551011

quantity :

0.1 mg

price :

280 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; 1:100	Chen W, Ho C, Chang Y, Chen H, Lin C, Ling T, et al. Cancer-associated fibroblasts regulate the plasticity of lung cancer stemness via paracrine signalling. Nat Commun. 2014;5:3472 pubmed publisher

product information

catalog number :

MBS551011

products type :

Antibody

products full name :

Rabbit anti IGF-II Polyclonal

products short name :

IGF-II

products name syn :

Rabbit anti Human IGF-II Polyclonal purified; IGF2; IGF-2; igf-ii; pab; polyclonal

other names :

insulin-like growth factor II isoform 1 preproprotein; Insulin-like growth factor II; Somatomedin-A

products gene name :

IGF-II

other gene names :

IGF2; IGF-II

uniprot entry name :

IGF2_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

180

purity :

>95% purity. Serum IgG fraction was purified by Protein A affinity chromatography.

form :

0.2um filtered 1 mg/ml solution in TBS pH 7.4, 50% glycerol, 1% BSA, 0.03% proclin.

storage stability :

This antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for 12 months without detectable loss of activity. Avoid repeated freeze/thaw cycles.

tested application :

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

app notes :

ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.

other info1 :

Source Note: Polyclonal IGF-II antibody was produced from sera of rabbits immunized with synthetic protein containing IGF-2 antigenic determinants.

other info2 :

Reconstitution: A quick spin of the vial followed by reconstitution in 0.01M PBS pH 7.4 or other diluents.

products categories :

Polyclonal

products description :

IGF-1 and IGF-2 are members of a protein family of related proteins with hormonal activities including nerve growth factor.Cell types responding to the two IGF factors include adipocytes, chondrocytes, epithelial cells, fibroblasts, glial cells, hepatocytes, muscle cells, and osteoblasts. The IGF-2 receptor, CD222, is a single-chain protein of 250 kDa. It is a high affinity binding protein for IGF-2 and shows very low affinity for IGF-1 and none for insulin. The cytoplasmic domain of the receptor does not possess intrinsic tyrosine kinase activity.

products references :

cell surface-bound il-1 is an upstream regulator of the senescence-associated il-6/ IL-8 cytokine network . PNAS, Sep 2009; 10.1073/pnas.0905299106. the angiogenic effect of probiotic bacillus polyfermenticus on human intestinal microvascular endothelial cells is mediated by IL-8 . Am J Physiol Gastrointest Liver Physiol, Sep 2009; 10.1152/ajpgi.00204.2009. IL-8 signaling does not mediate intra-amniotic lps-induced inflammation and maturation in preterm fetal lamb lung . Am J Physiol Lung Cell Mol Physiol, Sep 2009; 297: L512 - L519. the functional significance behind expressing two IL-8 receptor types on pmn . J. Leukoc. Biol., Sep 2009; 86: 529 - 543. IL-8 regulates mucin gene expression at the posttranscriptional level in lung epithelial cells . J. Immunol., Aug 2009; 183: 2159 - 2166.

ncbi gi num :

4504609

ncbi acc num :

NP_000603.1

ncbi gb acc num :

NM_000612.4

uniprot acc num :

P01344

ncbi mol weight :

20,140 Da

ncbi pathways :

Apoptosis Pathway (198797); Diabetes Pathways (105902); Endochondral Ossification Pathway (198812); Hedgehog Signaling Pathway (198835); Regulation Of Insulin-like Growth Factor (IGF) Activity By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway (105908)

ncbi summary :

This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

uniprot summary :

IGF2: The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development. Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SRS). A clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. The phenotypic expression changes during childhood and adolescence, with the facial features and asymmetry usually becoming more subtle with age. Belongs to the insulin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: extracellular space; extracellular region; plasma membrane. Molecular Function: insulin-like growth factor receptor binding; protein serine/threonine kinase activator activity; protein binding; growth factor activity; hormone activity; receptor activator activity; insulin receptor binding. Biological Process: positive regulation of catalytic activity; response to nicotine; genetic imprinting; multicellular organismal development; positive regulation of glycogen biosynthetic process; exocrine pancreas development; female pregnancy; positive regulation of activated T cell proliferation; response to estradiol stimulus; response to radiation; platelet degranulation; positive regulation of MAPKKK cascade; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; skeletal development; response to drug; response to nutrient levels; platelet activation; positive regulation of mitosis; glucose metabolic process; memory; osteoblast differentiation; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; organ morphogenesis; response to ethanol; cellular protein metabolic process; insulin receptor signaling pathway; striated muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of insulin receptor signaling pathway; positive regulation of protein amino acid phosphorylation; blood coagulation. Disease: Silver-russell Syndrome; Growth Restriction, Severe, With Distinctive Facies; Beckwith-wiedemann Syndrome; Wilms Tumor 1

size1 :

0.1 mg

price1 :

280 USD

size2 :

0.2 mg

price2 :

385

more info or order :

MyBioSource product webpage